Experiment: T7_phage 0.0015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ddg and ypdK are separated by 355 nucleotides ypdK and yfdZ are separated by 64 nucleotides yfdZ and ypdA are separated by 375 nucleotides
b2378: ddg - putative heat shock protein (VIMSS), at 2,493,667 to 2,494,587
ddg
b4680: ypdK - expressed protein (RefSeq), at 2,494,943 to 2,495,014
ypdK
b2379: yfdZ - hypothetical protein (NCBI), at 2,495,079 to 2,496,317
yfdZ
b2380: ypdA - predicted sensory kinase in two-component system with YpdB (NCBI), at 2,496,693 to 2,498,390
ypdA
Position (kb)
2495
2496
2497 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2494.277 kb on - strand, within ddg at 2494.430 kb on + strand, within ddg at 2494.446 kb on + strand, within ddg at 2494.446 kb on + strand, within ddg at 2494.522 kb on - strand at 2494.524 kb on + strand at 2494.527 kb on + strand at 2494.527 kb on + strand at 2494.527 kb on + strand at 2494.769 kb on + strand at 2494.795 kb on - strand at 2494.815 kb on - strand at 2494.883 kb on + strand at 2494.968 kb on + strand, within ypdK at 2495.161 kb on - strand at 2495.163 kb on - strand at 2495.163 kb on - strand at 2495.165 kb on + strand at 2495.358 kb on + strand, within yfdZ at 2495.371 kb on + strand, within yfdZ at 2495.404 kb on + strand, within yfdZ at 2495.412 kb on - strand, within yfdZ at 2495.412 kb on - strand, within yfdZ at 2495.444 kb on - strand, within yfdZ at 2495.444 kb on - strand, within yfdZ at 2495.480 kb on - strand, within yfdZ at 2495.480 kb on - strand, within yfdZ at 2495.682 kb on + strand, within yfdZ at 2495.690 kb on - strand, within yfdZ at 2495.865 kb on + strand, within yfdZ at 2496.012 kb on - strand, within yfdZ at 2496.051 kb on + strand, within yfdZ at 2496.139 kb on - strand, within yfdZ at 2496.247 kb on - strand at 2496.247 kb on - strand at 2496.282 kb on - strand at 2496.296 kb on + strand at 2496.348 kb on - strand at 2496.348 kb on - strand at 2496.448 kb on - strand at 2496.570 kb on + strand at 2496.570 kb on + strand at 2496.579 kb on - strand at 2496.655 kb on + strand at 2496.659 kb on + strand at 2496.687 kb on - strand at 2496.780 kb on - strand at 2496.814 kb on - strand at 2496.863 kb on + strand, within ypdA at 2496.863 kb on + strand, within ypdA at 2496.954 kb on + strand, within ypdA at 2497.033 kb on + strand at 2497.033 kb on + strand, within ypdA at 2497.065 kb on - strand, within ypdA at 2497.135 kb on - strand, within ypdA
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC1 remove 2,494,277 - ddg b2378 0.66 +0.1 2,494,430 + ddg b2378 0.83 -1.2 2,494,446 + ddg b2378 0.85 +1.6 2,494,446 + ddg b2378 0.85 +1.6 2,494,522 - -0.8 2,494,524 + -0.9 2,494,527 + +0.5 2,494,527 + +0.9 2,494,527 + -1.2 2,494,769 + -1.9 2,494,795 - +0.0 2,494,815 - -0.4 2,494,883 + -1.9 2,494,968 + ypdK b4680 0.35 +0.0 2,495,161 - +0.6 2,495,163 - -0.0 2,495,163 - -1.4 2,495,165 + -1.8 2,495,358 + yfdZ b2379 0.23 -1.5 2,495,371 + yfdZ b2379 0.24 -3.0 2,495,404 + yfdZ b2379 0.26 +0.3 2,495,412 - yfdZ b2379 0.27 +0.9 2,495,412 - yfdZ b2379 0.27 -0.8 2,495,444 - yfdZ b2379 0.29 -0.3 2,495,444 - yfdZ b2379 0.29 -0.2 2,495,480 - yfdZ b2379 0.32 +0.5 2,495,480 - yfdZ b2379 0.32 -0.4 2,495,682 + yfdZ b2379 0.49 -1.3 2,495,690 - yfdZ b2379 0.49 +0.2 2,495,865 + yfdZ b2379 0.63 -1.1 2,496,012 - yfdZ b2379 0.75 -0.6 2,496,051 + yfdZ b2379 0.78 -0.3 2,496,139 - yfdZ b2379 0.86 -1.6 2,496,247 - +0.1 2,496,247 - -0.3 2,496,282 - -0.6 2,496,296 + -1.8 2,496,348 - -4.0 2,496,348 - +1.4 2,496,448 - -0.4 2,496,570 + +0.8 2,496,570 + -0.6 2,496,579 - -0.1 2,496,655 + -0.7 2,496,659 + +0.5 2,496,687 - +2.7 2,496,780 - +0.7 2,496,814 - -0.5 2,496,863 + ypdA b2380 0.10 -2.2 2,496,863 + ypdA b2380 0.10 +0.1 2,496,954 + ypdA b2380 0.15 -0.4 2,497,033 + +0.2 2,497,033 + ypdA b2380 0.20 -0.2 2,497,065 - ypdA b2380 0.22 -0.4 2,497,135 - ypdA b2380 0.26 -0.3
Or see this region's nucleotide sequence