Strain Fitness in Escherichia coli BW25113 around b1963

Experiment: T7_phage 0.0015625 MOI TC1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdcm and yedJ are separated by 66 nucleotidesyedJ and b1963 are separated by 39 nucleotidesb1963 and rseX are separated by 164 nucleotidesrseX and yedS are separated by 311 nucleotides b1961: dcm - DNA cytosine methylase (NCBI), at 2,028,923 to 2,030,341 dcm b1962: yedJ - predicted phosphohydrolase (NCBI), at 2,030,408 to 2,031,103 yedJ b1963: b1963 - orf, hypothetical protein (VIMSS), at 2,031,143 to 2,031,508 b1963 b4603: rseX - sRNA regulating ompA and ompC translation, with Hfq (RefSeq), at 2,031,673 to 2,031,763 rseX b4496: yedS - no description, at 2,032,075 to 2,033,267 yedS Position (kb) 2031 2032Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2030.164 kb on + strand, within dcmat 2030.226 kb on + strandat 2030.226 kb on + strandat 2030.231 kb on + strandat 2030.451 kb on + strandat 2030.564 kb on + strand, within yedJat 2030.595 kb on + strand, within yedJat 2030.690 kb on + strand, within yedJat 2030.849 kb on + strand, within yedJat 2031.000 kb on + strand, within yedJat 2031.033 kb on - strand, within yedJat 2031.117 kb on + strandat 2031.157 kb on + strandat 2031.157 kb on + strandat 2031.174 kb on + strandat 2031.179 kb on + strandat 2031.179 kb on + strandat 2031.181 kb on - strand, within b1963at 2031.181 kb on - strand, within b1963at 2031.226 kb on + strand, within b1963at 2031.226 kb on + strand, within b1963at 2031.244 kb on + strand, within b1963at 2031.369 kb on - strand, within b1963at 2031.378 kb on + strand, within b1963at 2031.473 kb on + strandat 2031.507 kb on + strandat 2031.521 kb on + strandat 2031.522 kb on + strandat 2031.527 kb on - strandat 2031.527 kb on - strandat 2031.529 kb on - strandat 2031.697 kb on + strand, within rseXat 2031.776 kb on - strandat 2031.995 kb on - strandat 2031.995 kb on - strandat 2032.073 kb on - strandat 2032.173 kb on - strandat 2032.173 kb on - strandat 2032.181 kb on + strandat 2032.191 kb on - strandat 2032.191 kb on - strandat 2032.197 kb on + strand, within yedSat 2032.197 kb on + strand, within yedSat 2032.200 kb on + strand, within yedSat 2032.200 kb on + strand, within yedSat 2032.200 kb on + strand, within yedSat 2032.203 kb on - strand, within yedSat 2032.203 kb on - strand, within yedSat 2032.205 kb on + strand, within yedSat 2032.205 kb on + strand, within yedSat 2032.205 kb on + strand, within yedSat 2032.205 kb on - strand, within yedSat 2032.231 kb on + strand, within yedSat 2032.231 kb on + strand, within yedSat 2032.236 kb on + strand, within yedSat 2032.236 kb on - strand, within yedSat 2032.238 kb on + strand, within yedSat 2032.238 kb on + strand, within yedSat 2032.252 kb on - strand, within yedSat 2032.252 kb on - strand, within yedSat 2032.266 kb on + strand, within yedSat 2032.274 kb on + strand, within yedSat 2032.274 kb on - strand, within yedSat 2032.274 kb on - strand, within yedSat 2032.299 kb on - strand, within yedSat 2032.299 kb on - strand, within yedSat 2032.352 kb on - strand, within yedSat 2032.387 kb on + strand, within yedSat 2032.411 kb on + strand, within yedSat 2032.426 kb on + strand, within yedSat 2032.432 kb on - strand, within yedSat 2032.436 kb on + strand, within yedSat 2032.444 kb on - strand, within yedS

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC1
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2,030,164 + dcm b1961 0.87 -0.1
2,030,226 + +1.3
2,030,226 + -1.4
2,030,231 + -0.7
2,030,451 + +0.6
2,030,564 + yedJ b1962 0.22 +1.2
2,030,595 + yedJ b1962 0.27 -1.3
2,030,690 + yedJ b1962 0.41 -1.6
2,030,849 + yedJ b1962 0.63 -0.6
2,031,000 + yedJ b1962 0.85 +0.1
2,031,033 - yedJ b1962 0.90 -0.7
2,031,117 + +2.0
2,031,157 + +1.6
2,031,157 + -0.3
2,031,174 + -0.4
2,031,179 + -0.8
2,031,179 + +0.0
2,031,181 - b1963 b1963 0.10 +2.4
2,031,181 - b1963 b1963 0.10 +1.3
2,031,226 + b1963 b1963 0.23 +1.6
2,031,226 + b1963 b1963 0.23 +1.7
2,031,244 + b1963 b1963 0.28 -1.5
2,031,369 - b1963 b1963 0.62 +1.5
2,031,378 + b1963 b1963 0.64 +2.0
2,031,473 + -0.2
2,031,507 + +0.0
2,031,521 + -1.0
2,031,522 + +0.3
2,031,527 - -1.3
2,031,527 - +1.4
2,031,529 - +2.8
2,031,697 + rseX b4603 0.26 +0.7
2,031,776 - +0.4
2,031,995 - +0.0
2,031,995 - +0.7
2,032,073 - -1.0
2,032,173 - +1.4
2,032,173 - -0.7
2,032,181 + -0.2
2,032,191 - +0.2
2,032,191 - +1.0
2,032,197 + yedS b4496 0.10 +0.9
2,032,197 + yedS b4496 0.10 -1.8
2,032,200 + yedS b4496 0.10 +0.4
2,032,200 + yedS b4496 0.10 -0.4
2,032,200 + yedS b4496 0.10 +0.4
2,032,203 - yedS b4496 0.11 +0.0
2,032,203 - yedS b4496 0.11 +3.0
2,032,205 + yedS b4496 0.11 +1.0
2,032,205 + yedS b4496 0.11 -2.3
2,032,205 + yedS b4496 0.11 -0.5
2,032,205 - yedS b4496 0.11 -0.4
2,032,231 + yedS b4496 0.13 +1.7
2,032,231 + yedS b4496 0.13 +2.1
2,032,236 + yedS b4496 0.13 +1.1
2,032,236 - yedS b4496 0.13 -1.2
2,032,238 + yedS b4496 0.14 +0.2
2,032,238 + yedS b4496 0.14 +0.0
2,032,252 - yedS b4496 0.15 -1.3
2,032,252 - yedS b4496 0.15 -0.2
2,032,266 + yedS b4496 0.16 +1.0
2,032,274 + yedS b4496 0.17 -0.2
2,032,274 - yedS b4496 0.17 +0.9
2,032,274 - yedS b4496 0.17 -0.8
2,032,299 - yedS b4496 0.19 +0.8
2,032,299 - yedS b4496 0.19 +0.5
2,032,352 - yedS b4496 0.23 +1.2
2,032,387 + yedS b4496 0.26 -0.3
2,032,411 + yedS b4496 0.28 -0.0
2,032,426 + yedS b4496 0.29 +0.4
2,032,432 - yedS b4496 0.30 +0.3
2,032,436 + yedS b4496 0.30 -0.3
2,032,444 - yedS b4496 0.31 +0.0

Or see this region's nucleotide sequence