Experiment: Growth with Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1009 and PP_1010 are separated by 264 nucleotides PP_1010 and PP_1011 overlap by 4 nucleotides
PP_1009: PP_1009 - glyceraldehyde-3-phosphate dehydrogenase, at 1,150,010 to 1,151,011
_1009
PP_1010: PP_1010 - phosphogluconate dehydratase, at 1,151,276 to 1,153,102
_1010
PP_1011: PP_1011 - glucokinase, at 1,153,099 to 1,154,058
_1011
Position (kb)
1151
1152
1153
1154 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1150.435 kb on - strand, within PP_1009 at 1150.464 kb on + strand, within PP_1009 at 1150.464 kb on + strand, within PP_1009 at 1150.465 kb on - strand, within PP_1009 at 1150.465 kb on - strand, within PP_1009 at 1150.609 kb on - strand at 1150.610 kb on + strand, within PP_1009 at 1150.610 kb on + strand, within PP_1009 at 1150.611 kb on - strand, within PP_1009 at 1150.611 kb on - strand, within PP_1009 at 1150.611 kb on - strand, within PP_1009 at 1150.863 kb on + strand, within PP_1009 at 1150.864 kb on - strand, within PP_1009 at 1150.864 kb on - strand, within PP_1009 at 1150.864 kb on - strand, within PP_1009 at 1150.945 kb on + strand at 1150.946 kb on - strand at 1150.947 kb on + strand at 1150.947 kb on + strand at 1150.948 kb on - strand at 1151.017 kb on + strand at 1151.018 kb on - strand at 1151.018 kb on - strand at 1151.018 kb on - strand at 1151.196 kb on + strand at 1151.196 kb on + strand at 1151.252 kb on + strand at 1151.258 kb on + strand at 1153.123 kb on - strand at 1153.123 kb on - strand at 1153.178 kb on + strand at 1153.178 kb on + strand at 1153.179 kb on - strand at 1153.201 kb on + strand, within PP_1011 at 1153.202 kb on - strand, within PP_1011 at 1153.232 kb on - strand, within PP_1011 at 1153.752 kb on + strand, within PP_1011 at 1153.753 kb on - strand, within PP_1011 at 1153.839 kb on + strand, within PP_1011 at 1153.839 kb on + strand, within PP_1011 at 1153.839 kb on + strand, within PP_1011 at 1153.839 kb on + strand, within PP_1011 at 1153.839 kb on + strand, within PP_1011 at 1153.839 kb on + strand, within PP_1011 at 1153.840 kb on - strand, within PP_1011 at 1153.840 kb on - strand, within PP_1011 at 1153.873 kb on + strand, within PP_1011 at 1153.873 kb on + strand, within PP_1011 at 1153.873 kb on + strand, within PP_1011 at 1153.874 kb on - strand, within PP_1011 at 1153.874 kb on - strand, within PP_1011 at 1153.874 kb on - strand, within PP_1011 at 1153.874 kb on - strand, within PP_1011 at 1153.874 kb on - strand, within PP_1011 at 1153.875 kb on + strand, within PP_1011 at 1153.876 kb on - strand, within PP_1011 at 1153.876 kb on - strand, within PP_1011 at 1153.876 kb on - strand, within PP_1011 at 1153.956 kb on + strand, within PP_1011 at 1153.956 kb on + strand, within PP_1011 at 1153.956 kb on + strand, within PP_1011 at 1153.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM remove 1,150,435 - PP_1009 0.42 +0.4 1,150,464 + PP_1009 0.45 +0.2 1,150,464 + PP_1009 0.45 -0.6 1,150,465 - PP_1009 0.45 +0.5 1,150,465 - PP_1009 0.45 -1.7 1,150,609 - +1.1 1,150,610 + PP_1009 0.60 +0.2 1,150,610 + PP_1009 0.60 +1.9 1,150,611 - PP_1009 0.60 +0.6 1,150,611 - PP_1009 0.60 -1.2 1,150,611 - PP_1009 0.60 -0.3 1,150,863 + PP_1009 0.85 +0.3 1,150,864 - PP_1009 0.85 -1.5 1,150,864 - PP_1009 0.85 -1.0 1,150,864 - PP_1009 0.85 -1.7 1,150,945 + +0.8 1,150,946 - -2.7 1,150,947 + -0.7 1,150,947 + +0.5 1,150,948 - -2.5 1,151,017 + +1.5 1,151,018 - -2.1 1,151,018 - -2.7 1,151,018 - -1.1 1,151,196 + -2.1 1,151,196 + +0.4 1,151,252 + +0.9 1,151,258 + -2.7 1,153,123 - -0.9 1,153,123 - -2.3 1,153,178 + -0.7 1,153,178 + -2.3 1,153,179 - -0.4 1,153,201 + PP_1011 0.11 -2.1 1,153,202 - PP_1011 0.11 -0.2 1,153,232 - PP_1011 0.14 +0.8 1,153,752 + PP_1011 0.68 -2.6 1,153,753 - PP_1011 0.68 -2.3 1,153,839 + PP_1011 0.77 -1.0 1,153,839 + PP_1011 0.77 -0.5 1,153,839 + PP_1011 0.77 -1.6 1,153,839 + PP_1011 0.77 -1.8 1,153,839 + PP_1011 0.77 -2.4 1,153,839 + PP_1011 0.77 -1.5 1,153,840 - PP_1011 0.77 -1.1 1,153,840 - PP_1011 0.77 +0.6 1,153,873 + PP_1011 0.81 -1.7 1,153,873 + PP_1011 0.81 -0.6 1,153,873 + PP_1011 0.81 -1.7 1,153,874 - PP_1011 0.81 -0.7 1,153,874 - PP_1011 0.81 -1.0 1,153,874 - PP_1011 0.81 +1.1 1,153,874 - PP_1011 0.81 +0.6 1,153,874 - PP_1011 0.81 +0.0 1,153,875 + PP_1011 0.81 -2.3 1,153,876 - PP_1011 0.81 +0.8 1,153,876 - PP_1011 0.81 -0.7 1,153,876 - PP_1011 0.81 -0.7 1,153,956 + PP_1011 0.89 -1.8 1,153,956 + PP_1011 0.89 +0.3 1,153,956 + PP_1011 0.89 +0.3 1,153,966 + -2.6
Or see this region's nucleotide sequence