Strain Fitness in Escherichia coli BW25113 around b2487

Experiment: N4_phage 0.00009375 MOI TC5

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N4_phage 0.00009375 MOI TC5
2,605,521	-	hyfF	b2486	0.37	-0.8
2,605,579	+	hyfF	b2486	0.40	+0.2
2,605,601	-	hyfF	b2486	0.42	-1.0
2,605,601	-	hyfF	b2486	0.42	-0.9
2,605,686	-	hyfF	b2486	0.47	+0.3
2,605,686	-	hyfF	b2486	0.47	-1.2
2,605,829	-	hyfF	b2486	0.56	-2.2
2,605,969	+	hyfF	b2486	0.65	+1.5
2,605,969	+	hyfF	b2486	0.65	+0.4
2,606,180	+	hyfF	b2486	0.78	+0.1
2,606,240	-	hyfF	b2486	0.82	-0.5
2,606,420	-				-0.4
2,606,450	+				-0.5
2,606,611	-				+0.1
2,606,648	+				+2.5
2,606,761	-	hyfG	b2487	0.15	-0.7
2,606,893	-	hyfG	b2487	0.23	-0.6
2,606,922	+	hyfG	b2487	0.25	-0.5
2,606,922	+	hyfG	b2487	0.25	-2.4
2,606,990	+	hyfG	b2487	0.29	+0.5
2,606,998	-	hyfG	b2487	0.29	-0.2
2,606,998	-	hyfG	b2487	0.29	-0.7
2,607,008	-	hyfG	b2487	0.30	-1.5
2,607,199	+	hyfG	b2487	0.41	-0.6
2,607,250	+	hyfG	b2487	0.44	-0.1
2,607,250	+	hyfG	b2487	0.44	+0.8
2,607,258	+	hyfG	b2487	0.45	-0.9
2,607,260	+	hyfG	b2487	0.45	-0.2
2,607,264	+	hyfG	b2487	0.45	-0.3
2,607,272	-	hyfG	b2487	0.46	-1.5
2,607,354	+	hyfG	b2487	0.51	-0.7
2,607,362	+	hyfG	b2487	0.51	+1.0
2,607,397	-	hyfG	b2487	0.53	-0.7
2,607,548	+	hyfG	b2487	0.62	+0.6
2,607,838	-	hyfG	b2487	0.80	-1.3
2,607,879	+	hyfG	b2487	0.82	-2.9
2,608,003	-	hyfG	b2487	0.90	+0.3
2,608,030	-				-0.1
2,608,070	-				-0.4
2,608,089	-				-1.0
2,608,121	+				+0.2
2,608,121	+				+0.2
2,608,130	-				+1.2
2,608,223	-				-1.5
2,608,416	-	hyfH	b2488	0.42	-0.7
2,608,416	-	hyfH	b2488	0.42	+0.9
2,608,658	-	hyfH	b2488	0.86	+0.3
2,608,658	-	hyfH	b2488	0.86	-0.6
2,608,733	-				-0.2
2,608,733	-				-0.8
2,608,757	-				-0.0
2,609,013	+	hyfI	b2489	0.38	-0.9
2,609,013	+	hyfI	b2489	0.38	-0.2
2,609,095	-	hyfI	b2489	0.48	+1.0
2,609,097	+	hyfI	b2489	0.49	-0.0