Strain Fitness in Escherichia coli BW25113 around b1654

Experiment: N4_phage 0.00009375 MOI TC5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlhr and ydhD are separated by 50 nucleotidesydhD and ydhO are separated by 333 nucleotides b1653: lhr - predicted ATP-dependent helicase (NCBI), at 1,727,111 to 1,731,727 lhr b1654: ydhD - hypothetical protein (NCBI), at 1,731,778 to 1,732,125 ydhD b1655: ydhO - predicted lipoprotein (NCBI), at 1,732,459 to 1,733,274 ydhO Position (kb) 1731 1732 1733Strain fitness (log2 ratio) -1 0 1at 1731.154 kb on + strand, within lhrat 1731.316 kb on - strandat 1731.640 kb on - strandat 1731.728 kb on - strandat 1732.385 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC5
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1,731,154 + lhr b1653 0.88 -0.1
1,731,316 - -0.5
1,731,640 - +0.2
1,731,728 - +0.4
1,732,385 - +0.2

Or see this region's nucleotide sequence