Experiment: N4_phage 0.00009375 MOI TC5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcH and nfrA are separated by 0 nucleotides nfrA and nfrB overlap by 14 nucleotides
b0567: ybcH - hypothetical protein (NCBI), at 586,314 to 587,204
ybcH
b0568: nfrA - bacteriophage N4 receptor, outer membrane subunit (NCBI), at 587,205 to 590,177
nfrA
b0569: nfrB - bacteriophage N4 receptor, inner membrane subunit (NCBI), at 590,164 to 592,401
nfrB
Position (kb)
587
588
589
590
591 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 586.305 kb on - strand at 586.312 kb on + strand at 586.312 kb on + strand at 586.528 kb on - strand, within ybcH at 586.541 kb on + strand, within ybcH at 586.541 kb on - strand, within ybcH at 586.544 kb on - strand, within ybcH at 586.544 kb on - strand, within ybcH at 586.574 kb on + strand, within ybcH at 586.627 kb on - strand, within ybcH at 586.794 kb on + strand, within ybcH at 586.794 kb on + strand, within ybcH at 586.834 kb on - strand, within ybcH at 586.834 kb on - strand, within ybcH at 587.002 kb on - strand, within ybcH at 587.062 kb on + strand, within ybcH at 587.073 kb on + strand, within ybcH at 587.073 kb on + strand, within ybcH at 587.081 kb on - strand, within ybcH at 587.114 kb on + strand, within ybcH at 587.214 kb on - strand at 587.441 kb on + strand at 587.759 kb on + strand, within nfrA at 587.796 kb on - strand, within nfrA at 587.825 kb on - strand, within nfrA at 587.844 kb on + strand, within nfrA at 587.871 kb on + strand, within nfrA at 587.953 kb on + strand, within nfrA at 588.029 kb on - strand, within nfrA at 588.029 kb on - strand, within nfrA at 588.152 kb on - strand, within nfrA at 588.199 kb on + strand, within nfrA at 588.199 kb on + strand, within nfrA at 588.201 kb on - strand, within nfrA at 588.202 kb on + strand, within nfrA at 588.202 kb on + strand, within nfrA at 588.219 kb on + strand, within nfrA at 588.219 kb on + strand, within nfrA at 588.229 kb on + strand, within nfrA at 588.229 kb on + strand, within nfrA at 588.304 kb on - strand, within nfrA at 588.311 kb on + strand, within nfrA at 588.331 kb on + strand, within nfrA at 588.331 kb on + strand, within nfrA at 588.435 kb on - strand, within nfrA at 588.624 kb on - strand, within nfrA at 588.662 kb on - strand, within nfrA at 588.748 kb on - strand, within nfrA at 588.787 kb on + strand, within nfrA at 588.787 kb on + strand, within nfrA at 588.833 kb on - strand, within nfrA at 588.880 kb on + strand, within nfrA at 588.880 kb on + strand, within nfrA at 589.096 kb on - strand, within nfrA at 589.179 kb on + strand, within nfrA at 589.200 kb on - strand, within nfrA at 589.255 kb on - strand, within nfrA at 589.452 kb on + strand, within nfrA at 589.523 kb on + strand, within nfrA at 589.546 kb on + strand, within nfrA at 589.546 kb on + strand, within nfrA at 589.656 kb on + strand, within nfrA at 589.780 kb on + strand, within nfrA at 589.789 kb on - strand, within nfrA at 589.829 kb on - strand, within nfrA at 589.857 kb on - strand, within nfrA at 589.908 kb on + strand at 589.908 kb on + strand at 589.919 kb on + strand at 589.928 kb on - strand at 589.928 kb on - strand at 589.961 kb on - strand at 589.964 kb on + strand at 589.977 kb on + strand at 590.006 kb on - strand at 590.010 kb on + strand at 590.010 kb on + strand at 590.071 kb on - strand at 590.071 kb on - strand at 590.071 kb on - strand at 590.088 kb on + strand at 590.099 kb on + strand at 590.142 kb on - strand at 590.143 kb on - strand at 590.163 kb on - strand at 590.163 kb on - strand at 590.195 kb on - strand at 590.195 kb on - strand at 590.202 kb on + strand at 590.202 kb on + strand at 590.217 kb on - strand at 590.232 kb on - strand at 590.247 kb on + strand at 590.309 kb on - strand at 590.344 kb on - strand at 590.344 kb on - strand at 590.438 kb on - strand, within nfrB at 590.474 kb on + strand, within nfrB at 590.482 kb on - strand, within nfrB at 590.789 kb on + strand, within nfrB at 590.789 kb on + strand, within nfrB at 590.872 kb on + strand, within nfrB at 590.872 kb on + strand, within nfrB at 590.885 kb on - strand, within nfrB at 590.885 kb on - strand, within nfrB at 591.089 kb on + strand, within nfrB at 591.089 kb on + strand, within nfrB
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC5 remove 586,305 - +0.1 586,312 + -0.6 586,312 + +0.0 586,528 - ybcH b0567 0.24 +0.7 586,541 + ybcH b0567 0.25 +0.1 586,541 - ybcH b0567 0.25 -0.1 586,544 - ybcH b0567 0.26 -0.2 586,544 - ybcH b0567 0.26 -0.3 586,574 + ybcH b0567 0.29 +1.2 586,627 - ybcH b0567 0.35 -0.7 586,794 + ybcH b0567 0.54 +0.9 586,794 + ybcH b0567 0.54 +0.1 586,834 - ybcH b0567 0.58 -0.2 586,834 - ybcH b0567 0.58 +0.4 587,002 - ybcH b0567 0.77 +0.1 587,062 + ybcH b0567 0.84 +0.2 587,073 + ybcH b0567 0.85 +0.9 587,073 + ybcH b0567 0.85 +2.4 587,081 - ybcH b0567 0.86 +0.4 587,114 + ybcH b0567 0.90 +0.6 587,214 - +0.2 587,441 + +6.3 587,759 + nfrA b0568 0.19 +5.7 587,796 - nfrA b0568 0.20 +6.0 587,825 - nfrA b0568 0.21 +2.4 587,844 + nfrA b0568 0.21 +5.9 587,871 + nfrA b0568 0.22 +5.4 587,953 + nfrA b0568 0.25 +5.8 588,029 - nfrA b0568 0.28 +6.8 588,029 - nfrA b0568 0.28 +5.7 588,152 - nfrA b0568 0.32 +5.8 588,199 + nfrA b0568 0.33 +6.1 588,199 + nfrA b0568 0.33 +5.5 588,201 - nfrA b0568 0.34 +5.9 588,202 + nfrA b0568 0.34 +5.4 588,202 + nfrA b0568 0.34 +5.6 588,219 + nfrA b0568 0.34 +6.0 588,219 + nfrA b0568 0.34 +5.8 588,229 + nfrA b0568 0.34 +6.3 588,229 + nfrA b0568 0.34 +6.2 588,304 - nfrA b0568 0.37 +5.9 588,311 + nfrA b0568 0.37 +4.6 588,331 + nfrA b0568 0.38 +6.0 588,331 + nfrA b0568 0.38 +6.0 588,435 - nfrA b0568 0.41 +5.8 588,624 - nfrA b0568 0.48 +5.6 588,662 - nfrA b0568 0.49 +5.4 588,748 - nfrA b0568 0.52 +5.9 588,787 + nfrA b0568 0.53 +5.7 588,787 + nfrA b0568 0.53 +5.4 588,833 - nfrA b0568 0.55 +5.8 588,880 + nfrA b0568 0.56 +5.7 588,880 + nfrA b0568 0.56 +5.7 589,096 - nfrA b0568 0.64 +5.9 589,179 + nfrA b0568 0.66 +5.9 589,200 - nfrA b0568 0.67 +5.9 589,255 - nfrA b0568 0.69 +5.8 589,452 + nfrA b0568 0.76 +5.6 589,523 + nfrA b0568 0.78 +5.7 589,546 + nfrA b0568 0.79 +5.8 589,546 + nfrA b0568 0.79 +5.9 589,656 + nfrA b0568 0.82 +6.0 589,780 + nfrA b0568 0.87 +6.0 589,789 - nfrA b0568 0.87 +6.1 589,829 - nfrA b0568 0.88 +5.8 589,857 - nfrA b0568 0.89 +5.9 589,908 + +5.4 589,908 + +5.6 589,919 + +6.1 589,928 - +5.5 589,928 - +6.1 589,961 - +5.5 589,964 + +5.7 589,977 + +6.5 590,006 - +5.8 590,010 + +5.8 590,010 + +5.9 590,071 - +6.2 590,071 - +6.0 590,071 - +5.8 590,088 + +5.7 590,099 + +5.6 590,142 - +4.9 590,143 - +6.0 590,163 - +0.2 590,163 - -0.7 590,195 - +0.4 590,195 - +0.2 590,202 + +4.1 590,202 + +4.4 590,217 - +0.9 590,232 - +4.9 590,247 + +5.2 590,309 - +7.1 590,344 - +5.1 590,344 - +5.6 590,438 - nfrB b0569 0.12 +5.8 590,474 + nfrB b0569 0.14 +5.9 590,482 - nfrB b0569 0.14 +5.7 590,789 + nfrB b0569 0.28 +5.8 590,789 + nfrB b0569 0.28 +5.6 590,872 + nfrB b0569 0.32 +5.8 590,872 + nfrB b0569 0.32 +5.7 590,885 - nfrB b0569 0.32 +5.2 590,885 - nfrB b0569 0.32 +5.6 591,089 + nfrB b0569 0.41 +5.7 591,089 + nfrB b0569 0.41 +5.8
Or see this region's nucleotide sequence