Strain Fitness in Escherichia coli BW25113 around b3083

Experiment: N4_phage 0.00009375 MOI TC4

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N4_phage 0.00009375 MOI TC4
3,231,163	-	fadH	b3081	0.73	+0.7
3,231,199	-	fadH	b3081	0.75	+0.7
3,231,267	+	fadH	b3081	0.78	-1.2
3,231,267	+	fadH	b3081	0.78	-0.1
3,231,356	+	fadH	b3081	0.83	+0.2
3,231,399	-	fadH	b3081	0.85	+0.4
3,231,427	-	fadH	b3081	0.86	+0.6
3,231,428	+	fadH	b3081	0.86	+0.0
3,231,428	+	fadH	b3081	0.86	-0.7
3,231,428	+	fadH	b3081	0.86	+1.4
3,231,428	+	fadH	b3081	0.86	-3.7
3,231,504	-				-0.1
3,231,530	+				+0.6
3,231,530	+				-0.4
3,231,547	+				-0.1
3,231,552	+				-0.9
3,231,643	+				-0.4
3,231,673	+				+1.0
3,231,812	-	ygjM	b3082	0.15	-1.2
3,231,855	-	ygjM	b3082	0.25	+0.9
3,231,856	-	ygjM	b3082	0.25	-0.7
3,231,892	-	ygjM	b3082	0.34	+0.2
3,231,892	-	ygjM	b3082	0.34	-0.1
3,232,179	+				-0.6
3,232,179	+				+0.5
3,232,181	+				+0.5
3,232,186	+				-1.8
3,232,189	-				-2.3
3,232,233	+	ygjN	b3083	0.22	+0.4
3,232,267	+	ygjN	b3083	0.33	+0.6
3,232,272	-	ygjN	b3083	0.35	+2.0
3,232,302	+	ygjN	b3083	0.44	+0.8
3,232,394	+	ygjN	b3083	0.73	-0.3
3,232,416	+	ygjN	b3083	0.80	-0.2
3,232,465	-				+1.2
3,232,465	-				+0.2
3,232,465	-				-1.3
3,232,471	+				+0.9
3,232,603	+				-1.5
3,232,603	+				+1.6
3,232,704	-				+0.4
3,232,704	-				-1.5
3,232,783	+				+0.6
3,232,783	+				+0.2
3,232,815	+				-1.0
3,232,830	-				-0.7
3,232,862	+				-0.4
3,232,862	+				-0.6
3,232,924	-	ygjO	b3084	0.14	+1.1
3,232,967	-	ygjO	b3084	0.18	+0.0
3,232,979	-	ygjO	b3084	0.19	-2.3
3,232,979	-	ygjO	b3084	0.19	-0.3
3,232,999	-	ygjO	b3084	0.21	-2.1
3,232,999	-	ygjO	b3084	0.21	-0.9
3,233,263	-	ygjO	b3084	0.44	-0.6
3,233,263	-	ygjO	b3084	0.44	+0.5
3,233,289	+	ygjO	b3084	0.46	-0.2
3,233,297	-	ygjO	b3084	0.47	+0.7
3,233,297	-	ygjO	b3084	0.47	-1.3
3,233,297	-	ygjO	b3084	0.47	-0.3
3,233,352	-	ygjO	b3084	0.52	-0.5
3,233,352	-	ygjO	b3084	0.52	-0.7
3,233,358	-	ygjO	b3084	0.53	+0.3
3,233,358	-	ygjO	b3084	0.53	+0.8
3,233,402	+	ygjO	b3084	0.56	-0.9