Experiment: N4_phage 0.00009375 MOI TC4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcP and yfcQ overlap by 4 nucleotides yfcQ and yfcR overlap by 4 nucleotides yfcR and yfcS overlap by 1 nucleotides yfcS and yfcU are separated by 19 nucleotides
b2333: yfcP - predicted fimbrial-like adhesin protein (NCBI), at 2,448,073 to 2,448,612
yfcP
b2334: yfcQ - predicted fimbrial-like adhesin protein (NCBI), at 2,448,609 to 2,449,097
yfcQ
b2335: yfcR - predicted fimbrial-like adhesin protein (NCBI), at 2,449,094 to 2,449,606
yfcR
b2336: yfcS - predicted periplasmic pilus chaperone (NCBI), at 2,449,606 to 2,450,358
yfcS
b4661: yfcU - no description, at 2,450,378 to 2,453,023
yfcU
Position (kb)
2449
2450 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2448.112 kb on - strand at 2448.131 kb on - strand, within yfcP at 2448.145 kb on - strand, within yfcP at 2448.197 kb on - strand, within yfcP at 2448.197 kb on - strand, within yfcP at 2448.222 kb on - strand, within yfcP at 2448.222 kb on - strand, within yfcP at 2448.257 kb on + strand, within yfcP at 2448.272 kb on + strand, within yfcP at 2448.272 kb on + strand, within yfcP at 2448.288 kb on - strand, within yfcP at 2448.313 kb on + strand, within yfcP at 2448.315 kb on + strand, within yfcP at 2448.382 kb on + strand, within yfcP at 2448.382 kb on + strand, within yfcP at 2448.382 kb on + strand, within yfcP at 2448.398 kb on - strand, within yfcP at 2448.398 kb on - strand, within yfcP at 2448.405 kb on + strand, within yfcP at 2448.405 kb on + strand, within yfcP at 2448.413 kb on - strand, within yfcP at 2448.413 kb on - strand, within yfcP at 2448.438 kb on + strand, within yfcP at 2448.452 kb on + strand, within yfcP at 2448.467 kb on - strand, within yfcP at 2448.467 kb on - strand, within yfcP at 2448.478 kb on - strand, within yfcP at 2448.495 kb on - strand, within yfcP at 2448.495 kb on - strand, within yfcP at 2448.495 kb on - strand, within yfcP at 2448.519 kb on + strand, within yfcP at 2448.527 kb on - strand, within yfcP at 2448.527 kb on - strand, within yfcP at 2448.542 kb on + strand, within yfcP at 2448.554 kb on + strand, within yfcP at 2448.559 kb on + strand at 2448.567 kb on - strand at 2448.568 kb on + strand at 2448.568 kb on + strand at 2448.572 kb on + strand at 2448.580 kb on - strand at 2448.589 kb on + strand at 2448.632 kb on - strand at 2448.632 kb on - strand at 2448.657 kb on + strand at 2448.657 kb on + strand at 2448.665 kb on - strand, within yfcQ at 2448.750 kb on - strand, within yfcQ at 2448.787 kb on + strand, within yfcQ at 2448.826 kb on - strand, within yfcQ at 2448.826 kb on - strand, within yfcQ at 2448.841 kb on + strand, within yfcQ at 2448.841 kb on + strand, within yfcQ at 2448.841 kb on + strand, within yfcQ at 2448.841 kb on + strand, within yfcQ at 2448.846 kb on + strand, within yfcQ at 2448.849 kb on - strand, within yfcQ at 2448.864 kb on + strand, within yfcQ at 2448.891 kb on + strand, within yfcQ at 2449.084 kb on - strand at 2449.084 kb on - strand at 2449.108 kb on + strand at 2449.160 kb on + strand, within yfcR at 2449.160 kb on + strand, within yfcR at 2449.166 kb on + strand, within yfcR at 2449.166 kb on + strand, within yfcR at 2449.178 kb on - strand, within yfcR at 2449.240 kb on - strand, within yfcR at 2449.284 kb on - strand, within yfcR at 2449.293 kb on - strand, within yfcR at 2449.293 kb on - strand, within yfcR at 2449.297 kb on - strand, within yfcR at 2449.303 kb on - strand, within yfcR at 2449.323 kb on + strand, within yfcR at 2449.357 kb on - strand, within yfcR at 2449.359 kb on + strand, within yfcR at 2449.385 kb on + strand, within yfcR at 2449.418 kb on - strand, within yfcR at 2449.459 kb on + strand, within yfcR at 2449.459 kb on + strand, within yfcR at 2449.459 kb on + strand, within yfcR at 2449.464 kb on + strand, within yfcR at 2449.464 kb on + strand, within yfcR at 2449.469 kb on + strand, within yfcR at 2449.477 kb on - strand, within yfcR at 2449.482 kb on - strand, within yfcR at 2449.550 kb on + strand, within yfcR at 2449.555 kb on + strand at 2449.587 kb on + strand at 2449.587 kb on + strand at 2449.694 kb on - strand, within yfcS at 2449.828 kb on + strand, within yfcS at 2449.828 kb on + strand, within yfcS at 2449.873 kb on + strand, within yfcS at 2449.873 kb on + strand, within yfcS at 2449.890 kb on + strand, within yfcS at 2449.931 kb on + strand, within yfcS at 2449.939 kb on - strand, within yfcS at 2449.949 kb on - strand, within yfcS at 2450.018 kb on - strand, within yfcS at 2450.209 kb on - strand, within yfcS at 2450.211 kb on + strand, within yfcS at 2450.262 kb on - strand, within yfcS at 2450.327 kb on - strand at 2450.588 kb on - strand at 2450.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC4 remove 2,448,112 - +0.5 2,448,131 - yfcP b2333 0.11 +0.5 2,448,145 - yfcP b2333 0.13 +0.2 2,448,197 - yfcP b2333 0.23 +0.3 2,448,197 - yfcP b2333 0.23 -0.7 2,448,222 - yfcP b2333 0.28 +0.4 2,448,222 - yfcP b2333 0.28 +0.3 2,448,257 + yfcP b2333 0.34 +0.1 2,448,272 + yfcP b2333 0.37 -0.8 2,448,272 + yfcP b2333 0.37 -0.2 2,448,288 - yfcP b2333 0.40 -2.6 2,448,313 + yfcP b2333 0.44 -2.1 2,448,315 + yfcP b2333 0.45 -0.1 2,448,382 + yfcP b2333 0.57 +0.2 2,448,382 + yfcP b2333 0.57 -1.5 2,448,382 + yfcP b2333 0.57 +0.5 2,448,398 - yfcP b2333 0.60 +0.4 2,448,398 - yfcP b2333 0.60 -1.2 2,448,405 + yfcP b2333 0.61 -0.9 2,448,405 + yfcP b2333 0.61 +0.0 2,448,413 - yfcP b2333 0.63 -1.1 2,448,413 - yfcP b2333 0.63 -0.0 2,448,438 + yfcP b2333 0.68 -2.6 2,448,452 + yfcP b2333 0.70 -3.6 2,448,467 - yfcP b2333 0.73 -1.7 2,448,467 - yfcP b2333 0.73 -1.9 2,448,478 - yfcP b2333 0.75 -1.3 2,448,495 - yfcP b2333 0.78 +1.1 2,448,495 - yfcP b2333 0.78 -0.6 2,448,495 - yfcP b2333 0.78 +0.4 2,448,519 + yfcP b2333 0.83 +0.5 2,448,527 - yfcP b2333 0.84 -3.0 2,448,527 - yfcP b2333 0.84 -1.1 2,448,542 + yfcP b2333 0.87 -0.6 2,448,554 + yfcP b2333 0.89 +0.9 2,448,559 + +0.1 2,448,567 - -0.6 2,448,568 + +1.1 2,448,568 + -2.4 2,448,572 + -0.1 2,448,580 - -0.8 2,448,589 + +3.5 2,448,632 - +0.2 2,448,632 - -0.4 2,448,657 + +0.8 2,448,657 + -2.5 2,448,665 - yfcQ b2334 0.11 -0.3 2,448,750 - yfcQ b2334 0.29 -1.4 2,448,787 + yfcQ b2334 0.36 +0.2 2,448,826 - yfcQ b2334 0.44 -0.4 2,448,826 - yfcQ b2334 0.44 +1.1 2,448,841 + yfcQ b2334 0.47 -2.8 2,448,841 + yfcQ b2334 0.47 -0.3 2,448,841 + yfcQ b2334 0.47 -0.6 2,448,841 + yfcQ b2334 0.47 -2.7 2,448,846 + yfcQ b2334 0.48 +0.6 2,448,849 - yfcQ b2334 0.49 +1.1 2,448,864 + yfcQ b2334 0.52 -0.6 2,448,891 + yfcQ b2334 0.58 -1.0 2,449,084 - +0.1 2,449,084 - -1.8 2,449,108 + +0.9 2,449,160 + yfcR b2335 0.13 -0.7 2,449,160 + yfcR b2335 0.13 +0.2 2,449,166 + yfcR b2335 0.14 -0.3 2,449,166 + yfcR b2335 0.14 -1.0 2,449,178 - yfcR b2335 0.16 -1.0 2,449,240 - yfcR b2335 0.28 -0.4 2,449,284 - yfcR b2335 0.37 -0.4 2,449,293 - yfcR b2335 0.39 -1.2 2,449,293 - yfcR b2335 0.39 +0.1 2,449,297 - yfcR b2335 0.40 -0.1 2,449,303 - yfcR b2335 0.41 +0.6 2,449,323 + yfcR b2335 0.45 -2.0 2,449,357 - yfcR b2335 0.51 -0.8 2,449,359 + yfcR b2335 0.52 -1.8 2,449,385 + yfcR b2335 0.57 -0.9 2,449,418 - yfcR b2335 0.63 +0.0 2,449,459 + yfcR b2335 0.71 +0.3 2,449,459 + yfcR b2335 0.71 +0.9 2,449,459 + yfcR b2335 0.71 -3.2 2,449,464 + yfcR b2335 0.72 +1.5 2,449,464 + yfcR b2335 0.72 -2.4 2,449,469 + yfcR b2335 0.73 -1.0 2,449,477 - yfcR b2335 0.75 +0.2 2,449,482 - yfcR b2335 0.76 -0.6 2,449,550 + yfcR b2335 0.89 -1.3 2,449,555 + +0.0 2,449,587 + -0.2 2,449,587 + -1.1 2,449,694 - yfcS b2336 0.12 -0.6 2,449,828 + yfcS b2336 0.29 -2.6 2,449,828 + yfcS b2336 0.29 +1.0 2,449,873 + yfcS b2336 0.35 -0.6 2,449,873 + yfcS b2336 0.35 +0.2 2,449,890 + yfcS b2336 0.38 -0.6 2,449,931 + yfcS b2336 0.43 +0.1 2,449,939 - yfcS b2336 0.44 -0.5 2,449,949 - yfcS b2336 0.46 +1.4 2,450,018 - yfcS b2336 0.55 -3.7 2,450,209 - yfcS b2336 0.80 +0.6 2,450,211 + yfcS b2336 0.80 -1.8 2,450,262 - yfcS b2336 0.87 -0.4 2,450,327 - -2.5 2,450,588 - -0.2 2,450,589 - +0.5
Or see this region's nucleotide sequence