Strain Fitness in Escherichia coli BW25113 around b4661

Experiment: N4_phage 0.009375 MOI TC2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N4_phage 0.009375 MOI TC2
2,449,385	+	yfcR	b2335	0.57	+0.3
2,449,418	-	yfcR	b2335	0.63	-0.2
2,449,459	+	yfcR	b2335	0.71	+0.6
2,449,459	+	yfcR	b2335	0.71	+0.2
2,449,459	+	yfcR	b2335	0.71	-0.5
2,449,464	+	yfcR	b2335	0.72	-0.5
2,449,464	+	yfcR	b2335	0.72	-1.7
2,449,469	+	yfcR	b2335	0.73	-1.0
2,449,477	-	yfcR	b2335	0.75	-0.2
2,449,482	-	yfcR	b2335	0.76	+1.1
2,449,550	+	yfcR	b2335	0.89	+1.0
2,449,555	+				+0.1
2,449,587	+				-0.8
2,449,587	+				-3.1
2,449,694	-	yfcS	b2336	0.12	-0.2
2,449,828	+	yfcS	b2336	0.29	+0.1
2,449,828	+	yfcS	b2336	0.29	-2.0
2,449,873	+	yfcS	b2336	0.35	+2.0
2,449,873	+	yfcS	b2336	0.35	-0.8
2,449,890	+	yfcS	b2336	0.38	-0.5
2,449,931	+	yfcS	b2336	0.43	+0.3
2,449,939	-	yfcS	b2336	0.44	-0.7
2,449,949	-	yfcS	b2336	0.46	+0.2
2,450,018	-	yfcS	b2336	0.55	-2.0
2,450,209	-	yfcS	b2336	0.80	-0.1
2,450,211	+	yfcS	b2336	0.80	-0.2
2,450,262	-	yfcS	b2336	0.87	-0.2
2,450,327	-				-0.3
2,450,588	-				+0.0
2,450,589	-				-1.8
2,450,642	+				-1.4
2,450,686	+	yfcU	b4661	0.12	-1.0
2,450,709	-	yfcU	b4661	0.13	-0.8
2,450,786	+	yfcU	b4661	0.15	-0.7
2,450,990	+	yfcU	b4661	0.23	-0.9
2,451,034	+	yfcU	b4661	0.25	-2.1
2,451,054	+	yfcU	b4661	0.26	-0.8
2,451,054	+	yfcU	b4661	0.26	-1.0
2,451,077	-	yfcU	b4661	0.26	+0.6
2,451,077	-	yfcU	b4661	0.26	-0.1
2,451,228	-	yfcU	b4661	0.32	-1.7
2,451,301	-	yfcU	b4661	0.35	-0.8
2,451,302	-	yfcU	b4661	0.35	-2.2
2,451,333	+	yfcU	b4661	0.36	-0.7
2,451,434	-	yfcU	b4661	0.40	-1.7
2,451,443	-	yfcU	b4661	0.40	-1.2
2,451,459	-	yfcU	b4661	0.41	-1.2
2,451,473	+	yfcU	b4661	0.41	+0.6
2,451,500	-	yfcU	b4661	0.42	-0.9
2,451,523	+	yfcU	b4661	0.43	-0.4
2,451,523	+	yfcU	b4661	0.43	+0.5
2,451,712	+	yfcU	b4661	0.50	-0.9
2,451,761	+	yfcU	b4661	0.52	-2.2
2,451,782	-	yfcU	b4661	0.53	-0.3
2,451,875	-	yfcU	b4661	0.57	-0.4
2,451,939	-	yfcU	b4661	0.59	+0.4
2,451,939	-	yfcU	b4661	0.59	-0.7
2,452,001	+	yfcU	b4661	0.61	-0.7
2,452,035	-	yfcU	b4661	0.63	-2.0
2,452,095	-	yfcU	b4661	0.65	+1.8
2,452,095	-	yfcU	b4661	0.65	+0.0
2,452,108	+	yfcU	b4661	0.65	-3.0
2,452,141	+	yfcU	b4661	0.67	+1.0
2,452,279	-	yfcU	b4661	0.72	-0.4
2,452,400	-	yfcU	b4661	0.76	-0.6
2,452,449	-	yfcU	b4661	0.78	-0.6
2,452,485	+	yfcU	b4661	0.80	+0.1
2,452,488	+	yfcU	b4661	0.80	-0.2
2,452,499	-	yfcU	b4661	0.80	+0.3
2,452,504	-	yfcU	b4661	0.80	+1.1
2,452,509	+	yfcU	b4661	0.81	+0.6
2,452,521	+	yfcU	b4661	0.81	+3.2
2,452,563	-	yfcU	b4661	0.83	+1.2
2,452,563	-	yfcU	b4661	0.83	+1.6
2,452,640	-	yfcU	b4661	0.85	+2.4
2,452,673	-				+0.2
2,452,723	+	yfcU	b4661	0.89	-2.3
2,452,748	-	yfcU	b4661	0.90	+0.4
2,452,751	+	yfcU	b4661	0.90	+0.2
2,452,767	+				-1.9
2,452,768	+				+0.4
2,452,770	+				+0.1
2,452,770	+				-1.5
2,452,777	+				+0.2
2,452,777	+				+2.2
2,452,782	+				-1.2
2,452,782	+				-0.5
2,452,799	+				-2.2
2,452,809	+				-1.0
2,452,846	+				-0.2
2,452,853	+				-0.7
2,452,938	+				+0.1
2,452,939	+				-2.2
2,452,939	+				-0.7
2,452,945	-				-1.1
2,452,945	-				+0.0
2,452,945	-				-1.2
2,452,946	-				-1.2
2,452,946	-				+0.2
2,452,946	-				-0.8
2,452,964	-				-1.7
2,452,964	-				+1.0
2,452,980	+				-1.5
2,452,992	+				-1.6
2,453,015	+				-0.3
2,453,019	+				+1.6
2,453,064	+				+1.6
2,453,074	+				+0.9
2,453,074	+				+0.6
2,453,074	+				-1.1
2,453,087	-				-2.0
2,453,092	-				+2.2
2,453,094	+				+3.2
2,453,096	+				-0.1
2,453,097	-				+0.4
2,453,098	-				+0.6
2,453,102	+				-0.3
2,453,103	+				-0.1
2,453,103	+				+1.2
2,453,104	-				+0.9
2,453,107	+				+0.4
2,453,111	-				+0.2
2,453,111	-				+1.3
2,453,111	-				-0.1
2,453,111	-				-0.1
2,453,118	+				+3.2
2,453,127	-				-1.2
2,453,127	-				-1.6
2,453,144	+				+0.4
2,453,147	+				-0.1
2,453,160	-				+0.5
2,453,169	+	yfcV	b2339	0.11	+1.6
2,453,172	+	yfcV	b2339	0.12	+0.2
2,453,209	-	yfcV	b2339	0.18	+0.1
2,453,209	-	yfcV	b2339	0.18	+0.2
2,453,256	+	yfcV	b2339	0.27	-1.1
2,453,261	-	yfcV	b2339	0.28	+1.0
2,453,261	-	yfcV	b2339	0.28	-0.8
2,453,265	+	yfcV	b2339	0.28	-1.3
2,453,276	-	yfcV	b2339	0.30	+0.1
2,453,276	-	yfcV	b2339	0.30	-1.2
2,453,277	+	yfcV	b2339	0.30	-0.1
2,453,277	-	yfcV	b2339	0.30	+1.3
2,453,280	+	yfcV	b2339	0.31	-1.3
2,453,280	+	yfcV	b2339	0.31	-0.3
2,453,286	-	yfcV	b2339	0.32	+0.9
2,453,287	-	yfcV	b2339	0.32	+1.2
2,453,287	-	yfcV	b2339	0.32	-0.1
2,453,287	-	yfcV	b2339	0.32	+0.9
2,453,288	+	yfcV	b2339	0.32	+0.4
2,453,288	-	yfcV	b2339	0.32	-0.7
2,453,288	-	yfcV	b2339	0.32	-0.6
2,453,288	-	yfcV	b2339	0.32	+0.9
2,453,296	-	yfcV	b2339	0.34	-0.9
2,453,301	+	yfcV	b2339	0.35	-0.2
2,453,302	+	yfcV	b2339	0.35	+0.9
2,453,302	+	yfcV	b2339	0.35	-1.0
2,453,318	-	yfcV	b2339	0.38	+1.2
2,453,322	+	yfcV	b2339	0.38	-0.4
2,453,323	+	yfcV	b2339	0.39	-0.1
2,453,383	+	yfcV	b2339	0.49	+1.6
2,453,383	+	yfcV	b2339	0.49	+0.4
2,453,385	+	yfcV	b2339	0.50	+1.1
2,453,385	+	yfcV	b2339	0.50	-2.7
2,453,389	+	yfcV	b2339	0.50	-0.9
2,453,391	-	yfcV	b2339	0.51	+3.2
2,453,397	-	yfcV	b2339	0.52	+3.2
2,453,404	+	yfcV	b2339	0.53	-0.7
2,453,458	+	yfcV	b2339	0.63	-0.4
2,453,493	+	yfcV	b2339	0.69	+0.4
2,453,515	+	yfcV	b2339	0.73	-1.3
2,453,532	-	yfcV	b2339	0.76	+2.2
2,453,535	+	yfcV	b2339	0.76	+2.2
2,453,538	+	yfcV	b2339	0.77	+2.1
2,453,549	+	yfcV	b2339	0.79	-0.8
2,453,549	+	yfcV	b2339	0.79	+4.2
2,453,598	+	yfcV	b2339	0.87	+0.6
2,453,598	+	yfcV	b2339	0.87	+1.6
2,453,635	+				+0.5