Experiment: N4_phage 0.009375 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt basS and basR are separated by 9 nucleotides basR and yjdB overlap by 4 nucleotides yjdB and adiC are separated by 103 nucleotides
b4112: basS - sensory histidine kinase in two-component regulatory system with BasR (NCBI), at 4,330,204 to 4,331,295
basS
b4113: basR - DNA-binding response regulator in two-component regulatory system with BasS (NCBI), at 4,331,305 to 4,331,973
basR
b4114: yjdB - orf, hypothetical protein (VIMSS), at 4,331,970 to 4,333,613
yjdB
b4115: adiC - arginine:agmatin (NCBI), at 4,333,717 to 4,335,054
adiC
Position (kb)
4331
4332
4333
4334 Strain fitness (log2 ratio)
-2
-1
0
1 at 4331.007 kb on - strand, within basS at 4331.157 kb on - strand, within basS at 4331.159 kb on + strand, within basS at 4331.159 kb on + strand, within basS at 4331.201 kb on - strand at 4331.206 kb on - strand at 4331.351 kb on + strand at 4331.351 kb on + strand at 4331.359 kb on - strand at 4331.359 kb on - strand at 4331.359 kb on - strand at 4331.359 kb on - strand at 4331.359 kb on - strand at 4331.359 kb on - strand at 4331.359 kb on - strand at 4331.385 kb on + strand, within basR at 4331.393 kb on - strand, within basR at 4331.400 kb on + strand, within basR at 4331.444 kb on + strand, within basR at 4331.483 kb on - strand, within basR at 4331.487 kb on + strand, within basR at 4331.633 kb on - strand, within basR at 4331.644 kb on - strand, within basR at 4331.652 kb on - strand, within basR at 4331.748 kb on + strand, within basR at 4331.796 kb on + strand, within basR at 4331.814 kb on + strand, within basR at 4331.814 kb on + strand, within basR at 4331.816 kb on + strand, within basR at 4331.839 kb on + strand, within basR at 4331.839 kb on + strand, within basR at 4331.850 kb on + strand, within basR at 4331.850 kb on + strand, within basR at 4331.858 kb on - strand, within basR at 4331.858 kb on - strand, within basR at 4331.896 kb on + strand, within basR at 4331.975 kb on + strand at 4332.042 kb on - strand at 4332.042 kb on - strand at 4332.121 kb on + strand at 4332.121 kb on + strand at 4332.196 kb on + strand, within yjdB at 4332.208 kb on - strand at 4332.218 kb on - strand, within yjdB at 4332.218 kb on - strand, within yjdB at 4332.256 kb on - strand, within yjdB at 4332.320 kb on + strand, within yjdB at 4332.355 kb on - strand, within yjdB at 4332.379 kb on - strand, within yjdB at 4332.379 kb on - strand, within yjdB at 4332.379 kb on - strand, within yjdB at 4332.381 kb on + strand, within yjdB at 4332.388 kb on + strand, within yjdB at 4332.426 kb on - strand, within yjdB at 4332.526 kb on - strand, within yjdB at 4332.527 kb on + strand, within yjdB at 4332.557 kb on - strand, within yjdB at 4332.592 kb on - strand, within yjdB at 4332.597 kb on + strand, within yjdB at 4332.666 kb on - strand, within yjdB at 4332.666 kb on - strand, within yjdB at 4332.676 kb on + strand, within yjdB at 4332.676 kb on + strand, within yjdB at 4332.694 kb on + strand, within yjdB at 4332.711 kb on + strand, within yjdB at 4332.755 kb on + strand, within yjdB at 4332.790 kb on + strand, within yjdB at 4332.790 kb on + strand, within yjdB at 4332.987 kb on + strand, within yjdB at 4332.995 kb on - strand, within yjdB at 4332.995 kb on - strand, within yjdB at 4333.084 kb on + strand, within yjdB at 4333.113 kb on - strand, within yjdB at 4333.171 kb on - strand, within yjdB at 4333.171 kb on - strand, within yjdB at 4333.197 kb on + strand, within yjdB at 4333.197 kb on + strand, within yjdB at 4333.200 kb on + strand, within yjdB at 4333.227 kb on - strand, within yjdB at 4333.227 kb on - strand, within yjdB at 4333.259 kb on + strand, within yjdB at 4333.288 kb on + strand, within yjdB at 4333.300 kb on + strand, within yjdB at 4333.300 kb on + strand, within yjdB at 4333.300 kb on + strand, within yjdB at 4333.300 kb on + strand, within yjdB at 4333.300 kb on + strand, within yjdB at 4333.311 kb on + strand, within yjdB at 4333.311 kb on + strand, within yjdB at 4333.311 kb on + strand, within yjdB at 4333.311 kb on + strand, within yjdB at 4333.311 kb on + strand, within yjdB at 4333.324 kb on + strand, within yjdB at 4333.324 kb on + strand, within yjdB at 4333.331 kb on - strand, within yjdB at 4333.331 kb on - strand, within yjdB at 4333.361 kb on + strand, within yjdB at 4333.363 kb on + strand, within yjdB at 4333.363 kb on + strand, within yjdB at 4333.385 kb on - strand, within yjdB at 4333.385 kb on - strand, within yjdB at 4333.401 kb on - strand, within yjdB at 4333.401 kb on - strand, within yjdB at 4333.414 kb on + strand, within yjdB at 4333.421 kb on - strand, within yjdB at 4333.501 kb on + strand at 4333.501 kb on + strand at 4333.506 kb on + strand at 4333.511 kb on + strand at 4333.578 kb on + strand at 4333.578 kb on + strand at 4333.581 kb on - strand at 4333.630 kb on + strand at 4333.634 kb on + strand at 4333.638 kb on - strand at 4333.639 kb on + strand at 4333.644 kb on - strand at 4333.739 kb on + strand at 4333.745 kb on - strand at 4333.753 kb on - strand at 4333.798 kb on - strand at 4333.836 kb on + strand at 4333.861 kb on - strand, within adiC at 4333.861 kb on - strand, within adiC at 4333.877 kb on + strand, within adiC at 4333.905 kb on - strand, within adiC at 4333.918 kb on - strand, within adiC at 4333.936 kb on + strand, within adiC at 4333.936 kb on + strand, within adiC at 4333.944 kb on + strand, within adiC at 4333.950 kb on - strand, within adiC at 4333.951 kb on + strand, within adiC at 4333.951 kb on + strand, within adiC at 4333.951 kb on + strand, within adiC at 4334.027 kb on - strand, within adiC at 4334.134 kb on + strand, within adiC at 4334.156 kb on + strand, within adiC at 4334.156 kb on + strand, within adiC at 4334.246 kb on + strand, within adiC at 4334.300 kb on - strand, within adiC at 4334.300 kb on - strand, within adiC at 4334.371 kb on - strand, within adiC at 4334.382 kb on - strand, within adiC at 4334.411 kb on + strand, within adiC at 4334.429 kb on + strand, within adiC at 4334.468 kb on - strand, within adiC at 4334.469 kb on - strand, within adiC at 4334.498 kb on - strand, within adiC at 4334.501 kb on + strand, within adiC
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.009375 MOI TC2 remove 4,331,007 - basS b4112 0.74 -1.1 4,331,157 - basS b4112 0.87 -0.2 4,331,159 + basS b4112 0.87 -0.7 4,331,159 + basS b4112 0.87 -0.3 4,331,201 - +0.1 4,331,206 - +0.5 4,331,351 + +0.1 4,331,351 + +0.2 4,331,359 - -0.7 4,331,359 - -0.3 4,331,359 - +0.9 4,331,359 - -0.2 4,331,359 - -0.8 4,331,359 - -0.0 4,331,359 - -0.8 4,331,385 + basR b4113 0.12 +0.1 4,331,393 - basR b4113 0.13 +1.2 4,331,400 + basR b4113 0.14 +0.1 4,331,444 + basR b4113 0.21 -0.6 4,331,483 - basR b4113 0.27 +0.4 4,331,487 + basR b4113 0.27 -0.9 4,331,633 - basR b4113 0.49 -0.3 4,331,644 - basR b4113 0.51 +1.3 4,331,652 - basR b4113 0.52 -0.8 4,331,748 + basR b4113 0.66 +0.2 4,331,796 + basR b4113 0.73 -0.0 4,331,814 + basR b4113 0.76 +0.4 4,331,814 + basR b4113 0.76 +0.6 4,331,816 + basR b4113 0.76 +0.4 4,331,839 + basR b4113 0.80 -1.5 4,331,839 + basR b4113 0.80 -0.5 4,331,850 + basR b4113 0.81 -1.6 4,331,850 + basR b4113 0.81 -0.2 4,331,858 - basR b4113 0.83 -0.5 4,331,858 - basR b4113 0.83 -0.8 4,331,896 + basR b4113 0.88 +1.1 4,331,975 + -0.3 4,332,042 - +0.4 4,332,042 - +0.3 4,332,121 + +0.3 4,332,121 + +0.4 4,332,196 + yjdB b4114 0.14 +0.6 4,332,208 - +1.7 4,332,218 - yjdB b4114 0.15 -0.3 4,332,218 - yjdB b4114 0.15 +0.3 4,332,256 - yjdB b4114 0.17 +0.0 4,332,320 + yjdB b4114 0.21 +0.1 4,332,355 - yjdB b4114 0.23 -0.8 4,332,379 - yjdB b4114 0.25 +0.4 4,332,379 - yjdB b4114 0.25 -0.8 4,332,379 - yjdB b4114 0.25 +0.7 4,332,381 + yjdB b4114 0.25 +0.5 4,332,388 + yjdB b4114 0.25 +0.3 4,332,426 - yjdB b4114 0.28 +0.6 4,332,526 - yjdB b4114 0.34 +0.1 4,332,527 + yjdB b4114 0.34 +1.4 4,332,557 - yjdB b4114 0.36 +1.0 4,332,592 - yjdB b4114 0.38 +0.5 4,332,597 + yjdB b4114 0.38 -0.3 4,332,666 - yjdB b4114 0.42 +0.5 4,332,666 - yjdB b4114 0.42 +0.6 4,332,676 + yjdB b4114 0.43 +0.1 4,332,676 + yjdB b4114 0.43 -0.9 4,332,694 + yjdB b4114 0.44 +0.2 4,332,711 + yjdB b4114 0.45 -1.0 4,332,755 + yjdB b4114 0.48 -2.1 4,332,790 + yjdB b4114 0.50 -2.3 4,332,790 + yjdB b4114 0.50 -1.6 4,332,987 + yjdB b4114 0.62 +0.0 4,332,995 - yjdB b4114 0.62 -0.2 4,332,995 - yjdB b4114 0.62 +0.7 4,333,084 + yjdB b4114 0.68 +0.2 4,333,113 - yjdB b4114 0.70 +0.1 4,333,171 - yjdB b4114 0.73 +0.6 4,333,171 - yjdB b4114 0.73 -1.3 4,333,197 + yjdB b4114 0.75 +1.4 4,333,197 + yjdB b4114 0.75 +0.5 4,333,200 + yjdB b4114 0.75 -0.7 4,333,227 - yjdB b4114 0.76 +0.9 4,333,227 - yjdB b4114 0.76 -0.5 4,333,259 + yjdB b4114 0.78 -0.1 4,333,288 + yjdB b4114 0.80 +0.5 4,333,300 + yjdB b4114 0.81 +0.2 4,333,300 + yjdB b4114 0.81 +0.7 4,333,300 + yjdB b4114 0.81 +0.7 4,333,300 + yjdB b4114 0.81 +0.6 4,333,300 + yjdB b4114 0.81 +0.1 4,333,311 + yjdB b4114 0.82 +0.1 4,333,311 + yjdB b4114 0.82 -0.9 4,333,311 + yjdB b4114 0.82 -0.7 4,333,311 + yjdB b4114 0.82 -1.0 4,333,311 + yjdB b4114 0.82 -0.1 4,333,324 + yjdB b4114 0.82 -0.7 4,333,324 + yjdB b4114 0.82 +0.5 4,333,331 - yjdB b4114 0.83 +1.7 4,333,331 - yjdB b4114 0.83 +0.7 4,333,361 + yjdB b4114 0.85 +0.4 4,333,363 + yjdB b4114 0.85 -0.6 4,333,363 + yjdB b4114 0.85 -0.3 4,333,385 - yjdB b4114 0.86 +1.0 4,333,385 - yjdB b4114 0.86 +1.1 4,333,401 - yjdB b4114 0.87 -0.3 4,333,401 - yjdB b4114 0.87 -0.7 4,333,414 + yjdB b4114 0.88 -0.2 4,333,421 - yjdB b4114 0.88 -0.6 4,333,501 + -0.4 4,333,501 + +0.6 4,333,506 + +0.5 4,333,511 + -1.2 4,333,578 + +1.1 4,333,578 + -1.1 4,333,581 - +0.5 4,333,630 + +0.6 4,333,634 + +0.6 4,333,638 - -0.6 4,333,639 + +0.4 4,333,644 - -0.0 4,333,739 + -1.5 4,333,745 - -0.2 4,333,753 - -0.7 4,333,798 - +0.6 4,333,836 + -0.7 4,333,861 - adiC b4115 0.11 +0.4 4,333,861 - adiC b4115 0.11 +0.7 4,333,877 + adiC b4115 0.12 -0.4 4,333,905 - adiC b4115 0.14 -0.0 4,333,918 - adiC b4115 0.15 +0.1 4,333,936 + adiC b4115 0.16 +1.3 4,333,936 + adiC b4115 0.16 -0.7 4,333,944 + adiC b4115 0.17 +0.1 4,333,950 - adiC b4115 0.17 -0.4 4,333,951 + adiC b4115 0.17 +0.2 4,333,951 + adiC b4115 0.17 +0.6 4,333,951 + adiC b4115 0.17 -0.2 4,334,027 - adiC b4115 0.23 +0.6 4,334,134 + adiC b4115 0.31 +0.4 4,334,156 + adiC b4115 0.33 +0.3 4,334,156 + adiC b4115 0.33 +0.2 4,334,246 + adiC b4115 0.40 +0.8 4,334,300 - adiC b4115 0.44 +0.6 4,334,300 - adiC b4115 0.44 -0.9 4,334,371 - adiC b4115 0.49 -0.2 4,334,382 - adiC b4115 0.50 -0.2 4,334,411 + adiC b4115 0.52 -0.6 4,334,429 + adiC b4115 0.53 -0.1 4,334,468 - adiC b4115 0.56 -0.0 4,334,469 - adiC b4115 0.56 +0.1 4,334,498 - adiC b4115 0.58 +0.2 4,334,501 + adiC b4115 0.59 -2.5
Or see this region's nucleotide sequence