Experiment: N4_phage 0.009375 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt elaC and elaD are separated by 187 nucleotides elaD and yfbK are separated by 70 nucleotides
b2268: elaC - orf, hypothetical protein (VIMSS), at 2,379,630 to 2,380,547
elaC
b2269: elaD - predicted enzyme (NCBI), at 2,380,735 to 2,381,946
elaD
b2270: yfbK - hypothetical protein (NCBI), at 2,382,017 to 2,383,744
yfbK
Position (kb)
2380
2381
2382 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2379.761 kb on + strand, within elaC at 2379.778 kb on + strand, within elaC at 2379.829 kb on - strand, within elaC at 2379.915 kb on - strand, within elaC at 2379.915 kb on - strand, within elaC at 2380.020 kb on - strand, within elaC at 2380.022 kb on + strand, within elaC at 2380.022 kb on + strand, within elaC at 2380.022 kb on + strand, within elaC at 2380.051 kb on + strand, within elaC at 2380.202 kb on + strand, within elaC at 2380.202 kb on + strand, within elaC at 2380.266 kb on + strand, within elaC at 2380.313 kb on - strand, within elaC at 2380.313 kb on - strand, within elaC at 2380.369 kb on + strand, within elaC at 2380.373 kb on + strand, within elaC at 2380.375 kb on + strand, within elaC at 2380.383 kb on - strand, within elaC at 2380.391 kb on - strand, within elaC at 2380.395 kb on + strand, within elaC at 2380.395 kb on + strand, within elaC at 2380.395 kb on + strand, within elaC at 2380.396 kb on + strand, within elaC at 2380.409 kb on + strand, within elaC at 2380.409 kb on + strand, within elaC at 2380.453 kb on + strand, within elaC at 2380.457 kb on - strand at 2380.467 kb on + strand at 2380.467 kb on + strand at 2380.467 kb on + strand at 2380.484 kb on - strand at 2380.508 kb on + strand at 2380.508 kb on + strand at 2380.575 kb on + strand at 2380.589 kb on - strand at 2380.598 kb on + strand at 2380.603 kb on + strand at 2380.603 kb on + strand at 2380.603 kb on + strand at 2380.620 kb on - strand at 2380.656 kb on + strand at 2380.656 kb on + strand at 2380.707 kb on + strand at 2380.726 kb on + strand at 2380.731 kb on + strand at 2380.737 kb on + strand at 2380.825 kb on - strand at 2381.954 kb on - strand at 2381.988 kb on - strand at 2381.988 kb on - strand at 2381.999 kb on - strand at 2382.011 kb on - strand at 2382.011 kb on - strand at 2382.011 kb on - strand at 2382.020 kb on - strand at 2382.021 kb on - strand at 2382.025 kb on - strand at 2382.030 kb on - strand at 2382.034 kb on - strand at 2382.034 kb on - strand at 2382.035 kb on - strand at 2382.035 kb on - strand at 2382.035 kb on - strand at 2382.035 kb on - strand at 2382.037 kb on - strand at 2382.039 kb on - strand at 2382.045 kb on - strand at 2382.045 kb on - strand at 2382.045 kb on - strand at 2382.045 kb on - strand at 2382.047 kb on - strand at 2382.056 kb on - strand at 2382.083 kb on - strand at 2382.089 kb on - strand at 2382.125 kb on - strand at 2382.125 kb on - strand at 2382.125 kb on - strand at 2382.125 kb on - strand at 2382.138 kb on - strand at 2382.138 kb on - strand at 2382.141 kb on - strand at 2382.146 kb on - strand at 2382.159 kb on - strand at 2382.159 kb on - strand at 2382.159 kb on - strand at 2382.159 kb on - strand at 2382.169 kb on - strand at 2382.177 kb on + strand at 2382.183 kb on + strand at 2382.185 kb on - strand at 2382.215 kb on - strand, within yfbK at 2382.215 kb on - strand, within yfbK at 2382.217 kb on - strand, within yfbK at 2382.221 kb on + strand, within yfbK at 2382.228 kb on - strand, within yfbK at 2382.351 kb on - strand, within yfbK at 2382.361 kb on - strand, within yfbK at 2382.410 kb on + strand, within yfbK at 2382.420 kb on - strand, within yfbK at 2382.429 kb on - strand, within yfbK at 2382.429 kb on - strand, within yfbK at 2382.431 kb on - strand, within yfbK at 2382.431 kb on - strand, within yfbK at 2382.444 kb on - strand, within yfbK at 2382.489 kb on - strand, within yfbK at 2382.489 kb on - strand, within yfbK at 2382.489 kb on - strand, within yfbK at 2382.508 kb on - strand, within yfbK at 2382.529 kb on - strand, within yfbK at 2382.611 kb on - strand, within yfbK at 2382.611 kb on - strand, within yfbK at 2382.668 kb on - strand, within yfbK at 2382.688 kb on - strand, within yfbK at 2382.711 kb on - strand, within yfbK at 2382.714 kb on - strand, within yfbK at 2382.714 kb on - strand, within yfbK at 2382.856 kb on - strand, within yfbK at 2382.859 kb on + strand, within yfbK at 2382.867 kb on + strand, within yfbK at 2382.876 kb on + strand, within yfbK at 2382.876 kb on + strand, within yfbK at 2382.883 kb on - strand, within yfbK at 2382.893 kb on - strand, within yfbK at 2382.917 kb on - strand, within yfbK at 2382.917 kb on - strand, within yfbK at 2382.917 kb on - strand, within yfbK at 2382.930 kb on - strand, within yfbK at 2382.938 kb on - strand, within yfbK at 2382.939 kb on + strand, within yfbK
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.009375 MOI TC2 remove 2,379,761 + elaC b2268 0.14 -1.4 2,379,778 + elaC b2268 0.16 -2.4 2,379,829 - elaC b2268 0.22 -1.0 2,379,915 - elaC b2268 0.31 +0.3 2,379,915 - elaC b2268 0.31 +0.3 2,380,020 - elaC b2268 0.42 +0.8 2,380,022 + elaC b2268 0.43 +0.3 2,380,022 + elaC b2268 0.43 +0.5 2,380,022 + elaC b2268 0.43 +0.6 2,380,051 + elaC b2268 0.46 -1.0 2,380,202 + elaC b2268 0.62 -1.0 2,380,202 + elaC b2268 0.62 +0.2 2,380,266 + elaC b2268 0.69 +0.2 2,380,313 - elaC b2268 0.74 -0.2 2,380,313 - elaC b2268 0.74 +1.3 2,380,369 + elaC b2268 0.81 -0.3 2,380,373 + elaC b2268 0.81 -0.5 2,380,375 + elaC b2268 0.81 +2.1 2,380,383 - elaC b2268 0.82 -2.0 2,380,391 - elaC b2268 0.83 +2.6 2,380,395 + elaC b2268 0.83 -0.6 2,380,395 + elaC b2268 0.83 +0.1 2,380,395 + elaC b2268 0.83 -0.4 2,380,396 + elaC b2268 0.83 +0.0 2,380,409 + elaC b2268 0.85 -0.7 2,380,409 + elaC b2268 0.85 -1.6 2,380,453 + elaC b2268 0.90 +1.1 2,380,457 - +1.2 2,380,467 + -3.0 2,380,467 + +0.8 2,380,467 + -0.6 2,380,484 - +3.2 2,380,508 + +0.1 2,380,508 + +0.1 2,380,575 + +1.1 2,380,589 - +1.6 2,380,598 + +2.2 2,380,603 + +1.6 2,380,603 + +1.2 2,380,603 + +3.2 2,380,620 - +3.2 2,380,656 + +0.4 2,380,656 + +1.6 2,380,707 + +3.2 2,380,726 + +1.6 2,380,731 + +3.2 2,380,737 + -0.1 2,380,825 - +3.2 2,381,954 - +3.2 2,381,988 - +3.2 2,381,988 - +2.2 2,381,999 - -1.0 2,382,011 - +0.8 2,382,011 - +1.0 2,382,011 - -1.1 2,382,020 - -1.8 2,382,021 - -0.4 2,382,025 - +1.2 2,382,030 - +0.5 2,382,034 - -1.1 2,382,034 - +0.3 2,382,035 - +2.6 2,382,035 - +0.5 2,382,035 - +1.5 2,382,035 - +0.6 2,382,037 - -0.6 2,382,039 - -0.9 2,382,045 - -2.2 2,382,045 - -1.1 2,382,045 - +0.5 2,382,045 - +0.1 2,382,047 - -0.8 2,382,056 - -0.4 2,382,083 - +0.4 2,382,089 - +0.0 2,382,125 - -0.5 2,382,125 - -0.4 2,382,125 - -0.8 2,382,125 - +0.1 2,382,138 - -2.3 2,382,138 - -0.2 2,382,141 - -1.4 2,382,146 - +0.3 2,382,159 - -1.0 2,382,159 - +0.2 2,382,159 - -1.1 2,382,159 - +1.0 2,382,169 - -1.4 2,382,177 + -0.6 2,382,183 + +3.2 2,382,185 - -0.5 2,382,215 - yfbK b2270 0.11 +0.5 2,382,215 - yfbK b2270 0.11 +0.2 2,382,217 - yfbK b2270 0.12 +1.3 2,382,221 + yfbK b2270 0.12 +3.2 2,382,228 - yfbK b2270 0.12 +3.2 2,382,351 - yfbK b2270 0.19 -1.2 2,382,361 - yfbK b2270 0.20 +1.9 2,382,410 + yfbK b2270 0.23 +2.2 2,382,420 - yfbK b2270 0.23 +2.2 2,382,429 - yfbK b2270 0.24 +3.5 2,382,429 - yfbK b2270 0.24 +0.6 2,382,431 - yfbK b2270 0.24 -0.7 2,382,431 - yfbK b2270 0.24 -1.9 2,382,444 - yfbK b2270 0.25 -1.0 2,382,489 - yfbK b2270 0.27 +1.0 2,382,489 - yfbK b2270 0.27 +1.2 2,382,489 - yfbK b2270 0.27 +2.2 2,382,508 - yfbK b2270 0.28 +0.7 2,382,529 - yfbK b2270 0.30 +0.3 2,382,611 - yfbK b2270 0.34 -1.2 2,382,611 - yfbK b2270 0.34 +0.1 2,382,668 - yfbK b2270 0.38 -2.4 2,382,688 - yfbK b2270 0.39 +1.2 2,382,711 - yfbK b2270 0.40 +1.0 2,382,714 - yfbK b2270 0.40 -0.9 2,382,714 - yfbK b2270 0.40 -0.5 2,382,856 - yfbK b2270 0.49 -0.1 2,382,859 + yfbK b2270 0.49 +0.6 2,382,867 + yfbK b2270 0.49 +1.6 2,382,876 + yfbK b2270 0.50 -0.8 2,382,876 + yfbK b2270 0.50 -0.1 2,382,883 - yfbK b2270 0.50 -1.7 2,382,893 - yfbK b2270 0.51 -2.0 2,382,917 - yfbK b2270 0.52 +1.1 2,382,917 - yfbK b2270 0.52 -0.8 2,382,917 - yfbK b2270 0.52 +0.1 2,382,930 - yfbK b2270 0.53 -0.1 2,382,938 - yfbK b2270 0.53 +0.1 2,382,939 + yfbK b2270 0.53 +0.9
Or see this region's nucleotide sequence