Experiment: N4_phage 0.009375 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfF and ynfG are separated by 10 nucleotides ynfG and ynfH are separated by 1 nucleotides ynfH and b1591 are separated by 42 nucleotides b1591 and b1592 are separated by 194 nucleotides
b1588: ynfF - oxidoreductase subunit (RefSeq), at 1,658,580 to 1,661,003
ynfF
b1589: ynfG - oxidoreductase, Fe-S subunit (NCBI), at 1,661,014 to 1,661,631
ynfG
b1590: ynfH - oxidoreductase, membrane subunit (NCBI), at 1,661,633 to 1,662,487
ynfH
b1591: b1591 - putative oxidoreductase component (VIMSS), at 1,662,530 to 1,663,144
b1591
b1592: b1592 - putative chloride channel (VIMSS), at 1,663,339 to 1,664,595
b1592
Position (kb)
1661
1662
1663 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1660.766 kb on + strand at 1660.766 kb on + strand at 1660.779 kb on - strand at 1660.779 kb on - strand at 1660.790 kb on - strand at 1661.057 kb on - strand at 1661.346 kb on - strand, within ynfG at 1661.351 kb on + strand, within ynfG at 1661.351 kb on + strand, within ynfG at 1661.364 kb on - strand, within ynfG at 1661.755 kb on + strand, within ynfH at 1661.755 kb on + strand, within ynfH at 1661.782 kb on - strand, within ynfH at 1661.851 kb on - strand, within ynfH at 1662.281 kb on + strand, within ynfH at 1662.281 kb on + strand, within ynfH at 1662.405 kb on + strand at 1662.482 kb on + strand at 1662.522 kb on + strand at 1662.589 kb on + strand at 1662.796 kb on + strand, within b1591 at 1662.877 kb on + strand, within b1591
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.009375 MOI TC2 remove 1,660,766 + +1.9 1,660,766 + +0.4 1,660,779 - -1.5 1,660,779 - -1.6 1,660,790 - -0.3 1,661,057 - -1.7 1,661,346 - ynfG b1589 0.54 -1.9 1,661,351 + ynfG b1589 0.55 -1.8 1,661,351 + ynfG b1589 0.55 -0.1 1,661,364 - ynfG b1589 0.57 -0.5 1,661,755 + ynfH b1590 0.14 -2.3 1,661,755 + ynfH b1590 0.14 -2.5 1,661,782 - ynfH b1590 0.17 -0.3 1,661,851 - ynfH b1590 0.25 -0.1 1,662,281 + ynfH b1590 0.76 -1.0 1,662,281 + ynfH b1590 0.76 +0.4 1,662,405 + -1.5 1,662,482 + +1.7 1,662,522 + -2.6 1,662,589 + +0.5 1,662,796 + b1591 b1591 0.43 +1.0 1,662,877 + b1591 b1591 0.56 -1.6
Or see this region's nucleotide sequence