Experiment: N4_phage 0.009375 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lit and intE are separated by 90 nucleotides intE and ymfG overlap by 20 nucleotides ymfG and ymfI are separated by 464 nucleotides
b1139: lit - e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI), at 1,197,918 to 1,198,811
lit
b1140: intE - e14 prophage; predicted integrase (NCBI), at 1,198,902 to 1,200,029
intE
b1141: ymfG - e14 prophage; predicted excisionase (NCBI), at 1,200,010 to 1,200,255
ymfG
b1143: ymfI - orf, hypothetical protein (VIMSS), at 1,200,720 to 1,201,061
ymfI
b1144: ymfJ - orf, hypothetical protein (VIMSS), at 1,200,999 to 1,201,307
ymfJ
Position (kb)
1198
1199
1200
1201 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1198.462 kb on + strand, within lit at 1198.531 kb on + strand, within lit at 1198.539 kb on - strand, within lit at 1198.539 kb on - strand, within lit at 1198.560 kb on - strand, within lit at 1198.564 kb on + strand, within lit at 1198.564 kb on + strand, within lit at 1198.572 kb on - strand, within lit at 1198.618 kb on + strand, within lit at 1198.620 kb on - strand, within lit at 1198.625 kb on - strand, within lit at 1198.626 kb on - strand, within lit at 1198.626 kb on - strand, within lit at 1198.657 kb on - strand, within lit at 1198.657 kb on - strand, within lit at 1198.677 kb on + strand, within lit at 1198.681 kb on - strand, within lit at 1198.684 kb on - strand, within lit at 1198.684 kb on - strand, within lit at 1198.688 kb on + strand, within lit at 1198.693 kb on - strand, within lit at 1198.695 kb on - strand, within lit at 1198.696 kb on - strand, within lit at 1198.816 kb on - strand at 1198.884 kb on - strand at 1198.915 kb on - strand at 1198.915 kb on - strand at 1198.915 kb on - strand at 1198.932 kb on - strand at 1198.988 kb on - strand at 1199.013 kb on - strand at 1199.056 kb on - strand, within intE at 1199.056 kb on - strand, within intE at 1199.086 kb on - strand, within intE at 1199.086 kb on - strand, within intE at 1199.130 kb on - strand, within intE at 1199.130 kb on - strand, within intE at 1199.145 kb on - strand, within intE at 1199.147 kb on + strand, within intE at 1199.155 kb on - strand, within intE at 1199.177 kb on + strand, within intE at 1199.177 kb on + strand, within intE at 1199.177 kb on + strand, within intE at 1199.185 kb on - strand, within intE at 1199.185 kb on - strand, within intE at 1199.185 kb on - strand, within intE at 1199.187 kb on + strand, within intE at 1199.187 kb on + strand, within intE at 1199.188 kb on + strand, within intE at 1199.189 kb on + strand, within intE at 1199.195 kb on - strand, within intE at 1199.197 kb on + strand, within intE at 1199.199 kb on - strand, within intE at 1199.207 kb on + strand, within intE at 1199.207 kb on + strand, within intE at 1199.208 kb on - strand, within intE at 1199.216 kb on - strand, within intE at 1199.295 kb on + strand, within intE at 1199.369 kb on + strand, within intE at 1199.369 kb on + strand, within intE at 1199.378 kb on + strand, within intE at 1199.378 kb on - strand, within intE at 1199.379 kb on - strand, within intE at 1199.446 kb on - strand, within intE at 1199.492 kb on + strand, within intE at 1199.505 kb on + strand, within intE at 1199.534 kb on - strand, within intE at 1199.550 kb on + strand, within intE at 1199.600 kb on + strand, within intE at 1199.631 kb on - strand, within intE at 1199.631 kb on - strand, within intE at 1199.681 kb on - strand, within intE at 1199.692 kb on + strand, within intE at 1199.692 kb on + strand, within intE at 1199.736 kb on - strand, within intE at 1199.742 kb on + strand, within intE at 1199.742 kb on + strand, within intE at 1199.870 kb on - strand, within intE at 1199.943 kb on - strand at 1199.943 kb on - strand at 1200.021 kb on + strand at 1200.088 kb on + strand, within ymfG at 1200.127 kb on - strand, within ymfG at 1200.191 kb on - strand, within ymfG at 1200.236 kb on + strand at 1200.278 kb on - strand at 1200.298 kb on + strand at 1200.340 kb on - strand at 1200.347 kb on + strand at 1200.360 kb on - strand at 1200.368 kb on - strand at 1200.404 kb on - strand at 1200.475 kb on - strand at 1200.475 kb on - strand at 1200.482 kb on + strand at 1200.683 kb on - strand at 1200.770 kb on - strand, within ymfI at 1200.919 kb on + strand, within ymfI
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.009375 MOI TC2 remove 1,198,462 + lit b1139 0.61 +3.4 1,198,531 + lit b1139 0.69 +3.4 1,198,539 - lit b1139 0.69 +2.4 1,198,539 - lit b1139 0.69 -0.0 1,198,560 - lit b1139 0.72 +1.0 1,198,564 + lit b1139 0.72 +3.4 1,198,564 + lit b1139 0.72 +2.4 1,198,572 - lit b1139 0.73 +1.9 1,198,618 + lit b1139 0.78 +3.4 1,198,620 - lit b1139 0.79 +0.1 1,198,625 - lit b1139 0.79 +1.4 1,198,626 - lit b1139 0.79 +0.3 1,198,626 - lit b1139 0.79 +0.1 1,198,657 - lit b1139 0.83 -1.0 1,198,657 - lit b1139 0.83 -1.3 1,198,677 + lit b1139 0.85 +3.4 1,198,681 - lit b1139 0.85 -0.6 1,198,684 - lit b1139 0.86 +0.3 1,198,684 - lit b1139 0.86 +2.4 1,198,688 + lit b1139 0.86 +3.4 1,198,693 - lit b1139 0.87 -2.1 1,198,695 - lit b1139 0.87 -0.8 1,198,696 - lit b1139 0.87 +2.1 1,198,816 - -1.1 1,198,884 - +1.4 1,198,915 - -1.2 1,198,915 - -0.5 1,198,915 - -1.0 1,198,932 - +1.1 1,198,988 - +0.3 1,199,013 - +1.1 1,199,056 - intE b1140 0.14 +0.8 1,199,056 - intE b1140 0.14 +0.5 1,199,086 - intE b1140 0.16 +0.0 1,199,086 - intE b1140 0.16 +0.9 1,199,130 - intE b1140 0.20 +0.7 1,199,130 - intE b1140 0.20 -0.7 1,199,145 - intE b1140 0.22 -1.0 1,199,147 + intE b1140 0.22 +0.3 1,199,155 - intE b1140 0.22 +1.2 1,199,177 + intE b1140 0.24 -0.0 1,199,177 + intE b1140 0.24 -0.7 1,199,177 + intE b1140 0.24 -0.2 1,199,185 - intE b1140 0.25 -2.2 1,199,185 - intE b1140 0.25 -0.2 1,199,185 - intE b1140 0.25 +1.2 1,199,187 + intE b1140 0.25 -0.4 1,199,187 + intE b1140 0.25 -1.3 1,199,188 + intE b1140 0.25 -1.1 1,199,189 + intE b1140 0.25 -0.3 1,199,195 - intE b1140 0.26 -1.9 1,199,197 + intE b1140 0.26 -1.6 1,199,199 - intE b1140 0.26 +1.2 1,199,207 + intE b1140 0.27 -1.2 1,199,207 + intE b1140 0.27 -0.8 1,199,208 - intE b1140 0.27 -1.7 1,199,216 - intE b1140 0.28 -0.4 1,199,295 + intE b1140 0.35 -0.4 1,199,369 + intE b1140 0.41 -0.1 1,199,369 + intE b1140 0.41 +0.9 1,199,378 + intE b1140 0.42 +0.9 1,199,378 - intE b1140 0.42 -1.1 1,199,379 - intE b1140 0.42 -0.4 1,199,446 - intE b1140 0.48 +0.1 1,199,492 + intE b1140 0.52 -0.6 1,199,505 + intE b1140 0.53 -0.0 1,199,534 - intE b1140 0.56 -0.1 1,199,550 + intE b1140 0.57 -2.0 1,199,600 + intE b1140 0.62 +0.1 1,199,631 - intE b1140 0.65 -2.6 1,199,631 - intE b1140 0.65 +0.1 1,199,681 - intE b1140 0.69 -0.6 1,199,692 + intE b1140 0.70 -1.1 1,199,692 + intE b1140 0.70 -0.9 1,199,736 - intE b1140 0.74 -1.6 1,199,742 + intE b1140 0.74 -0.3 1,199,742 + intE b1140 0.74 -1.3 1,199,870 - intE b1140 0.86 -0.9 1,199,943 - +0.1 1,199,943 - -1.2 1,200,021 + -0.4 1,200,088 + ymfG b1141 0.32 -1.2 1,200,127 - ymfG b1141 0.48 -0.7 1,200,191 - ymfG b1141 0.74 -0.3 1,200,236 + -0.7 1,200,278 - -0.1 1,200,298 + +0.8 1,200,340 - -0.4 1,200,347 + -1.3 1,200,360 - +0.6 1,200,368 - -1.7 1,200,404 - +0.4 1,200,475 - -2.4 1,200,475 - -0.7 1,200,482 + -1.6 1,200,683 - -2.8 1,200,770 - ymfI b1143 0.15 +0.5 1,200,919 + ymfI b1143 0.58 -1.6
Or see this region's nucleotide sequence