Experiment: Growth with Chloride 500 mM; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1935 and PP_5487 are separated by 154 nucleotides PP_5487 and PP_5488 are separated by 13 nucleotides PP_5488 and PP_5489 are separated by 16 nucleotides PP_5489 and PP_5490 are separated by 173 nucleotides
PP_1935: PP_1935 - Transcriptional regulator, Cro/CI family, at 2,181,727 to 2,182,035
_1935
PP_5487: PP_5487 - conserved protein of unknown function, at 2,182,190 to 2,182,582
_5487
PP_5488: PP_5488 - conserved protein of unknown function, at 2,182,596 to 2,183,255
_5488
PP_5489: PP_5489 - conserved protein of unknown function, at 2,183,272 to 2,183,769
_5489
PP_5490: PP_5490 - site-specific DNA recombinase, at 2,183,943 to 2,184,509
_5490
Position (kb)
2182
2183
2184 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2181.610 kb on + strand at 2181.645 kb on + strand at 2181.646 kb on - strand at 2181.655 kb on + strand at 2181.656 kb on - strand at 2181.668 kb on - strand at 2181.678 kb on + strand at 2181.719 kb on + strand at 2181.729 kb on - strand at 2181.885 kb on - strand, within PP_1935 at 2181.911 kb on + strand, within PP_1935 at 2181.949 kb on - strand, within PP_1935 at 2182.069 kb on - strand at 2182.132 kb on - strand at 2182.186 kb on - strand at 2182.194 kb on + strand at 2182.195 kb on - strand at 2182.359 kb on + strand, within PP_5487 at 2182.408 kb on + strand, within PP_5487 at 2182.413 kb on - strand, within PP_5487 at 2182.446 kb on + strand, within PP_5487 at 2182.580 kb on + strand at 2182.698 kb on + strand, within PP_5488 at 2182.743 kb on + strand, within PP_5488 at 2182.743 kb on + strand, within PP_5488 at 2182.909 kb on - strand, within PP_5488 at 2182.938 kb on + strand, within PP_5488 at 2182.938 kb on + strand, within PP_5488 at 2182.940 kb on + strand, within PP_5488 at 2183.189 kb on + strand, within PP_5488 at 2183.191 kb on + strand at 2183.192 kb on - strand at 2183.192 kb on - strand at 2183.195 kb on + strand at 2183.253 kb on + strand at 2183.254 kb on - strand at 2183.258 kb on + strand at 2183.259 kb on - strand at 2183.507 kb on - strand, within PP_5489 at 2183.535 kb on + strand, within PP_5489 at 2183.536 kb on - strand, within PP_5489 at 2183.592 kb on + strand, within PP_5489 at 2183.597 kb on + strand, within PP_5489 at 2183.597 kb on + strand, within PP_5489 at 2183.743 kb on - strand at 2183.789 kb on - strand at 2183.828 kb on - strand at 2183.942 kb on + strand at 2183.992 kb on + strand at 2184.004 kb on + strand, within PP_5490 at 2184.167 kb on + strand, within PP_5490 at 2184.167 kb on + strand, within PP_5490 at 2184.168 kb on - strand, within PP_5490 at 2184.236 kb on + strand, within PP_5490 at 2184.237 kb on - strand, within PP_5490
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM; with MOPS remove 2,181,610 + -0.7 2,181,645 + +0.4 2,181,646 - -0.8 2,181,655 + +0.2 2,181,656 - +0.4 2,181,668 - -0.2 2,181,678 + +2.0 2,181,719 + +0.6 2,181,729 - -0.0 2,181,885 - PP_1935 0.51 -2.5 2,181,911 + PP_1935 0.60 -0.1 2,181,949 - PP_1935 0.72 +0.2 2,182,069 - -0.7 2,182,132 - +0.6 2,182,186 - -3.4 2,182,194 + +0.6 2,182,195 - -1.3 2,182,359 + PP_5487 0.43 -0.6 2,182,408 + PP_5487 0.55 -0.7 2,182,413 - PP_5487 0.57 -1.3 2,182,446 + PP_5487 0.65 +0.8 2,182,580 + +0.3 2,182,698 + PP_5488 0.15 +2.1 2,182,743 + PP_5488 0.22 +0.3 2,182,743 + PP_5488 0.22 -0.2 2,182,909 - PP_5488 0.47 +0.8 2,182,938 + PP_5488 0.52 +0.1 2,182,938 + PP_5488 0.52 +1.7 2,182,940 + PP_5488 0.52 +1.7 2,183,189 + PP_5488 0.90 +1.0 2,183,191 + +0.1 2,183,192 - -1.1 2,183,192 - +1.4 2,183,195 + +0.3 2,183,253 + -0.0 2,183,254 - -3.4 2,183,258 + -2.5 2,183,259 - -0.0 2,183,507 - PP_5489 0.47 +1.1 2,183,535 + PP_5489 0.53 +0.5 2,183,536 - PP_5489 0.53 +1.9 2,183,592 + PP_5489 0.64 -3.2 2,183,597 + PP_5489 0.65 -1.0 2,183,597 + PP_5489 0.65 -1.3 2,183,743 - -4.0 2,183,789 - +1.0 2,183,828 - -1.5 2,183,942 + -0.2 2,183,992 + +0.8 2,184,004 + PP_5490 0.11 -1.7 2,184,167 + PP_5490 0.40 -0.4 2,184,167 + PP_5490 0.40 +0.4 2,184,168 - PP_5490 0.40 -2.5 2,184,236 + PP_5490 0.52 -1.0 2,184,237 - PP_5490 0.52 +1.2
Or see this region's nucleotide sequence