Experiment: Growth with Chloride 500 mM; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0363 and PP_0364 overlap by 8 nucleotides PP_0364 and PP_0365 overlap by 8 nucleotides PP_0365 and PP_0366 are separated by 10 nucleotides PP_0366 and PP_0367 are separated by 173 nucleotides
PP_0363: PP_0363 - 8-amino-7-oxononanoate synthase, at 441,120 to 442,292
_0363
PP_0364: PP_0364 - pimeloyl-[acp] methyl ester esterase, at 442,285 to 443,016
_0364
PP_0365: PP_0365 - Malonyl-[acyl-carrier protein] O-methyltransferase, at 443,009 to 443,827
_0365
PP_0366: PP_0366 - dethiobiotin synthetase, at 443,838 to 444,518
_0366
PP_0367: PP_0367 - conserved protein of unknown function, at 444,692 to 445,027
_0367
Position (kb) 443
444 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 442.237 kb on + strand at 442.238 kb on - strand at 442.238 kb on - strand at 442.238 kb on - strand at 442.290 kb on + strand at 442.290 kb on + strand at 442.291 kb on - strand at 442.304 kb on + strand at 442.304 kb on + strand at 442.305 kb on - strand at 442.305 kb on - strand at 442.305 kb on - strand at 442.385 kb on + strand, within PP_0364 at 442.650 kb on + strand, within PP_0364 at 442.650 kb on + strand, within PP_0364 at 442.650 kb on + strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.651 kb on - strand, within PP_0364 at 442.660 kb on - strand, within PP_0364 at 442.677 kb on + strand, within PP_0364 at 442.677 kb on + strand, within PP_0364 at 442.678 kb on - strand, within PP_0364 at 442.759 kb on + strand, within PP_0364 at 442.759 kb on + strand, within PP_0364 at 442.788 kb on + strand, within PP_0364 at 442.819 kb on + strand, within PP_0364 at 442.819 kb on + strand, within PP_0364 at 442.862 kb on + strand, within PP_0364 at 442.863 kb on - strand, within PP_0364 at 442.911 kb on - strand, within PP_0364 at 442.911 kb on - strand, within PP_0364 at 442.957 kb on + strand at 443.097 kb on - strand, within PP_0365 at 443.127 kb on + strand, within PP_0365 at 443.312 kb on + strand, within PP_0365 at 443.312 kb on + strand, within PP_0365 at 443.313 kb on - strand, within PP_0365 at 443.455 kb on - strand, within PP_0365 at 443.455 kb on - strand, within PP_0365 at 443.535 kb on + strand, within PP_0365 at 443.536 kb on - strand, within PP_0365 at 443.536 kb on - strand, within PP_0365 at 443.536 kb on - strand, within PP_0365 at 443.561 kb on + strand, within PP_0365 at 443.562 kb on - strand, within PP_0365 at 443.562 kb on - strand, within PP_0365 at 443.562 kb on - strand, within PP_0365 at 443.622 kb on + strand, within PP_0365 at 443.622 kb on + strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.623 kb on - strand, within PP_0365 at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.747 kb on + strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.748 kb on - strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.786 kb on + strand at 443.787 kb on - strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.798 kb on + strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.799 kb on - strand at 443.826 kb on - strand at 444.043 kb on - strand, within PP_0366 at 444.043 kb on - strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.077 kb on + strand, within PP_0366 at 444.078 kb on - strand, within PP_0366 at 444.080 kb on - strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.184 kb on + strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.185 kb on - strand, within PP_0366 at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.517 kb on - strand at 444.559 kb on + strand at 444.594 kb on - strand at 444.630 kb on + strand at 444.631 kb on - strand at 444.659 kb on + strand at 444.659 kb on + strand at 444.679 kb on + strand at 444.690 kb on - strand at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.758 kb on + strand, within PP_0367 at 444.759 kb on - strand, within PP_0367 at 444.759 kb on - strand, within PP_0367
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM; with MOPS remove 442,237 + -3.2 442,238 - -1.8 442,238 - -1.1 442,238 - -1.6 442,290 + -0.9 442,290 + +1.5 442,291 - -3.1 442,304 + -2.6 442,304 + -3.0 442,305 - -1.0 442,305 - -1.4 442,305 - -0.8 442,385 + PP_0364 0.14 -4.2 442,650 + PP_0364 0.50 -1.9 442,650 + PP_0364 0.50 -1.7 442,650 + PP_0364 0.50 -3.2 442,651 - PP_0364 0.50 -1.2 442,651 - PP_0364 0.50 -2.4 442,651 - PP_0364 0.50 -4.3 442,651 - PP_0364 0.50 -3.9 442,660 - PP_0364 0.51 -1.7 442,677 + PP_0364 0.54 -0.3 442,677 + PP_0364 0.54 -0.9 442,678 - PP_0364 0.54 -2.1 442,759 + PP_0364 0.65 -1.9 442,759 + PP_0364 0.65 -2.7 442,788 + PP_0364 0.69 -2.3 442,819 + PP_0364 0.73 -2.1 442,819 + PP_0364 0.73 -2.3 442,862 + PP_0364 0.79 -3.9 442,863 - PP_0364 0.79 -1.9 442,911 - PP_0364 0.86 -2.1 442,911 - PP_0364 0.86 -1.7 442,957 + +1.2 443,097 - PP_0365 0.11 -1.2 443,127 + PP_0365 0.14 -3.1 443,312 + PP_0365 0.37 -1.3 443,312 + PP_0365 0.37 -2.1 443,313 - PP_0365 0.37 -4.0 443,455 - PP_0365 0.54 -3.9 443,455 - PP_0365 0.54 -1.1 443,535 + PP_0365 0.64 -4.3 443,536 - PP_0365 0.64 -3.0 443,536 - PP_0365 0.64 -3.7 443,536 - PP_0365 0.64 -0.6 443,561 + PP_0365 0.67 -4.0 443,562 - PP_0365 0.68 -2.1 443,562 - PP_0365 0.68 -1.9 443,562 - PP_0365 0.68 -1.8 443,622 + PP_0365 0.75 -1.9 443,622 + PP_0365 0.75 -2.4 443,623 - PP_0365 0.75 +0.3 443,623 - PP_0365 0.75 -2.4 443,623 - PP_0365 0.75 -2.9 443,623 - PP_0365 0.75 -0.3 443,623 - PP_0365 0.75 -0.7 443,623 - PP_0365 0.75 -0.9 443,623 - PP_0365 0.75 -3.2 443,623 - PP_0365 0.75 -1.7 443,623 - PP_0365 0.75 -2.8 443,623 - PP_0365 0.75 -1.6 443,623 - PP_0365 0.75 -2.3 443,747 + -2.6 443,747 + -2.8 443,747 + -2.4 443,747 + -2.2 443,747 + -2.2 443,748 - -1.5 443,748 - -3.8 443,748 - -1.9 443,748 - -2.5 443,786 + -4.2 443,786 + -0.3 443,786 + -3.7 443,787 - -1.8 443,798 + +0.3 443,798 + -3.4 443,798 + -0.9 443,798 + -2.8 443,798 + -2.9 443,798 + -1.7 443,799 - -3.2 443,799 - -2.5 443,799 - -1.9 443,799 - -3.6 443,799 - -1.5 443,799 - -2.5 443,826 - -0.4 444,043 - PP_0366 0.30 -2.9 444,043 - PP_0366 0.30 -1.6 444,077 + PP_0366 0.35 -1.7 444,077 + PP_0366 0.35 -4.8 444,078 - PP_0366 0.35 +0.5 444,080 - PP_0366 0.36 -2.4 444,184 + PP_0366 0.51 -3.5 444,184 + PP_0366 0.51 -3.4 444,185 - PP_0366 0.51 -2.6 444,185 - PP_0366 0.51 -1.2 444,185 - PP_0366 0.51 -1.1 444,517 - -3.5 444,517 - +0.2 444,517 - +0.6 444,517 - +1.3 444,559 + -0.2 444,594 - -0.2 444,630 + -0.8 444,631 - +1.2 444,659 + +1.3 444,659 + +0.3 444,679 + +0.4 444,690 - -0.2 444,758 + PP_0367 0.20 -0.4 444,758 + PP_0367 0.20 +0.7 444,758 + PP_0367 0.20 -1.3 444,758 + PP_0367 0.20 -1.4 444,758 + PP_0367 0.20 -0.2 444,759 - PP_0367 0.20 +1.0 444,759 - PP_0367 0.20 +0.3
Or see this region's nucleotide sequence