Strain Fitness in Pseudomonas putida KT2440 around PP_0294

Experiment: Growth with Chloride 500 mM; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0292 and PP_0293 are separated by 10 nucleotidesPP_0293 and PP_0294 are separated by 117 nucleotidesPP_0294 and PP_0295 overlap by 4 nucleotidesPP_0295 and PP_0296 are separated by 84 nucleotides PP_0292: PP_0292 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data), at 351,515 to 352,252 _0292 PP_0293: PP_0293 - Imidazole glycerol phosphate synthase subunit HisF, at 352,263 to 353,033 _0293 PP_0294: PP_0294 - Choline / carnitine / glycine betaine ABC transporter, ATPase component (from data), at 353,151 to 354,329 _0294 PP_0295: PP_0295 - choline / betaine / carnitine ABC transporter - membrane subunit, at 354,326 to 355,171 _0295 PP_0296: PP_0296 - choline / betaine / carnitine ABC transporter - substrate binding protein, at 355,256 to 356,203 _0296 Position (kb) 353 354 355Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 352.193 kb on + strandat 352.193 kb on + strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.241 kb on + strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.242 kb on - strandat 352.457 kb on + strand, within PP_0293at 352.457 kb on + strand, within PP_0293at 352.457 kb on + strand, within PP_0293at 352.977 kb on + strandat 352.977 kb on + strandat 352.978 kb on - strandat 353.001 kb on + strandat 353.002 kb on - strandat 353.002 kb on - strandat 353.427 kb on - strand, within PP_0294at 353.519 kb on + strand, within PP_0294at 353.542 kb on + strand, within PP_0294at 353.542 kb on + strand, within PP_0294at 353.543 kb on - strand, within PP_0294at 353.543 kb on - strand, within PP_0294at 353.618 kb on + strand, within PP_0294at 353.618 kb on + strand, within PP_0294at 354.113 kb on + strand, within PP_0294at 354.114 kb on - strand, within PP_0294at 354.114 kb on - strand, within PP_0294at 354.368 kb on - strandat 354.428 kb on - strand, within PP_0295at 354.577 kb on + strand, within PP_0295at 354.655 kb on - strand, within PP_0295at 354.739 kb on + strand, within PP_0295at 354.750 kb on + strand, within PP_0295at 354.802 kb on + strand, within PP_0295at 354.803 kb on - strandat 354.804 kb on + strand, within PP_0295at 354.804 kb on + strand, within PP_0295at 354.804 kb on + strand, within PP_0295at 354.804 kb on + strand, within PP_0295at 354.804 kb on + strand, within PP_0295at 354.805 kb on - strand, within PP_0295at 354.805 kb on - strand, within PP_0295at 354.805 kb on - strand, within PP_0295at 354.860 kb on + strand, within PP_0295at 354.860 kb on + strand, within PP_0295at 354.861 kb on - strand, within PP_0295at 354.861 kb on - strand, within PP_0295at 354.861 kb on - strand, within PP_0295at 355.137 kb on + strandat 355.137 kb on + strandat 355.137 kb on + strandat 355.137 kb on + strandat 355.138 kb on - strandat 355.151 kb on + strandat 355.152 kb on - strandat 355.165 kb on + strandat 355.174 kb on + strandat 355.193 kb on + strandat 355.193 kb on + strandat 355.193 kb on + strandat 355.193 kb on + strandat 355.194 kb on - strandat 355.194 kb on - strandat 355.242 kb on + strandat 355.243 kb on - strandat 355.243 kb on - strandat 355.249 kb on + strandat 355.249 kb on + strandat 355.249 kb on + strandat 355.249 kb on + strandat 355.250 kb on - strandat 355.264 kb on + strandat 355.264 kb on + strandat 355.264 kb on + strandat 355.265 kb on - strandat 355.265 kb on - strandat 355.265 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM; with MOPS
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352,193 + -2.4
352,193 + -3.5
352,194 - -1.5
352,194 - -3.3
352,194 - -2.5
352,194 - -1.4
352,241 + -2.8
352,242 - -2.7
352,242 - -2.6
352,242 - -2.2
352,242 - -2.2
352,242 - -2.5
352,242 - -2.2
352,457 + PP_0293 0.25 -3.5
352,457 + PP_0293 0.25 -1.5
352,457 + PP_0293 0.25 -3.3
352,977 + -2.1
352,977 + -2.3
352,978 - -2.5
353,001 + -2.6
353,002 - -2.7
353,002 - -3.3
353,427 - PP_0294 0.23 +0.4
353,519 + PP_0294 0.31 -1.2
353,542 + PP_0294 0.33 -1.0
353,542 + PP_0294 0.33 -1.7
353,543 - PP_0294 0.33 -0.7
353,543 - PP_0294 0.33 +0.6
353,618 + PP_0294 0.40 -0.6
353,618 + PP_0294 0.40 -0.5
354,113 + PP_0294 0.82 -0.8
354,114 - PP_0294 0.82 -1.3
354,114 - PP_0294 0.82 +1.0
354,368 - +0.4
354,428 - PP_0295 0.12 +0.2
354,577 + PP_0295 0.30 +0.8
354,655 - PP_0295 0.39 -0.0
354,739 + PP_0295 0.49 -0.2
354,750 + PP_0295 0.50 +0.8
354,802 + PP_0295 0.56 -2.2
354,803 - +2.4
354,804 + PP_0295 0.57 +0.5
354,804 + PP_0295 0.57 -0.9
354,804 + PP_0295 0.57 +0.4
354,804 + PP_0295 0.57 +1.5
354,804 + PP_0295 0.57 -1.4
354,805 - PP_0295 0.57 +0.5
354,805 - PP_0295 0.57 +0.1
354,805 - PP_0295 0.57 +2.0
354,860 + PP_0295 0.63 -1.0
354,860 + PP_0295 0.63 -1.1
354,861 - PP_0295 0.63 +1.2
354,861 - PP_0295 0.63 -1.1
354,861 - PP_0295 0.63 -0.3
355,137 + -1.4
355,137 + -2.6
355,137 + -2.5
355,137 + +0.7
355,138 - -0.8
355,151 + -2.6
355,152 - +0.6
355,165 + +0.5
355,174 + -1.5
355,193 + +0.8
355,193 + +0.0
355,193 + +0.1
355,193 + +0.9
355,194 - +2.4
355,194 - +0.6
355,242 + +0.4
355,243 - -0.2
355,243 - +1.0
355,249 + -0.7
355,249 + -1.0
355,249 + -0.9
355,249 + -0.3
355,250 - +1.0
355,264 + +0.1
355,264 + +1.1
355,264 + -0.1
355,265 - -0.7
355,265 - +1.0
355,265 - +1.9

Or see this region's nucleotide sequence