Strain Fitness in Pseudomonas putida KT2440 around PP_1449

Experiment: Growth with Chloride 500 mM; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1447 and PP_1448 are separated by 1 nucleotidesPP_1448 and PP_1449 are separated by 160 nucleotidesPP_1449 and PP_1450 are separated by 27 nucleotides PP_1447: PP_1447 - conserved protein of unknown function, at 1,651,196 to 1,651,444 _1447 PP_1448: PP_1448 - conserved membrane protein of unknown function, at 1,651,446 to 1,652,081 _1448 PP_1449: PP_1449 - conserved exported protein of unknown function, at 1,652,242 to 1,656,768 _1449 PP_1450: PP_1450 - putative Activation/secretion protein, TPS family, at 1,656,796 to 1,658,493 _1450 Position (kb) 1652 1653 1654 1655 1656 1657Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1651.295 kb on + strand, within PP_1447at 1651.296 kb on - strand, within PP_1447at 1651.296 kb on - strand, within PP_1447at 1651.296 kb on - strand, within PP_1447at 1651.418 kb on + strand, within PP_1447at 1651.418 kb on + strand, within PP_1447at 1651.419 kb on - strand, within PP_1447at 1651.419 kb on - strand, within PP_1447at 1651.520 kb on - strand, within PP_1448at 1651.703 kb on + strand, within PP_1448at 1651.704 kb on - strand, within PP_1448at 1651.725 kb on + strand, within PP_1448at 1651.726 kb on - strand, within PP_1448at 1651.813 kb on - strand, within PP_1448at 1651.813 kb on - strand, within PP_1448at 1651.813 kb on - strand, within PP_1448at 1651.813 kb on - strand, within PP_1448at 1651.821 kb on + strand, within PP_1448at 1651.821 kb on + strand, within PP_1448at 1651.821 kb on + strand, within PP_1448at 1651.822 kb on - strand, within PP_1448at 1651.822 kb on - strand, within PP_1448at 1651.822 kb on - strand, within PP_1448at 1651.822 kb on - strand, within PP_1448at 1651.896 kb on + strand, within PP_1448at 1651.938 kb on + strand, within PP_1448at 1651.938 kb on + strand, within PP_1448at 1651.938 kb on + strand, within PP_1448at 1651.939 kb on - strand, within PP_1448at 1651.939 kb on - strand, within PP_1448at 1651.939 kb on - strand, within PP_1448at 1651.939 kb on - strand, within PP_1448at 1651.975 kb on - strand, within PP_1448at 1652.087 kb on - strandat 1652.148 kb on - strandat 1652.148 kb on - strandat 1652.243 kb on + strandat 1652.244 kb on - strandat 1652.289 kb on + strandat 1652.290 kb on - strandat 1652.497 kb on + strandat 1652.497 kb on + strandat 1652.498 kb on - strandat 1652.560 kb on - strandat 1652.717 kb on + strand, within PP_1449at 1652.717 kb on + strand, within PP_1449at 1652.717 kb on + strand, within PP_1449at 1652.829 kb on + strand, within PP_1449at 1652.913 kb on + strand, within PP_1449at 1652.913 kb on + strand, within PP_1449at 1652.947 kb on + strand, within PP_1449at 1653.247 kb on + strand, within PP_1449at 1653.247 kb on + strand, within PP_1449at 1653.373 kb on + strand, within PP_1449at 1653.373 kb on + strand, within PP_1449at 1653.373 kb on + strand, within PP_1449at 1653.374 kb on - strandat 1653.374 kb on - strand, within PP_1449at 1653.448 kb on + strand, within PP_1449at 1653.449 kb on - strand, within PP_1449at 1653.449 kb on - strand, within PP_1449at 1653.449 kb on - strand, within PP_1449at 1653.521 kb on + strand, within PP_1449at 1653.521 kb on + strand, within PP_1449at 1653.522 kb on - strand, within PP_1449at 1653.696 kb on - strand, within PP_1449at 1653.718 kb on + strand, within PP_1449at 1653.719 kb on - strand, within PP_1449at 1653.740 kb on + strand, within PP_1449at 1653.741 kb on - strand, within PP_1449at 1653.860 kb on + strand, within PP_1449at 1653.860 kb on + strand, within PP_1449at 1653.861 kb on - strand, within PP_1449at 1653.861 kb on - strand, within PP_1449at 1653.861 kb on - strand, within PP_1449at 1654.188 kb on - strand, within PP_1449at 1654.209 kb on + strand, within PP_1449at 1654.504 kb on + strand, within PP_1449at 1654.505 kb on - strand, within PP_1449at 1654.505 kb on - strand, within PP_1449at 1654.698 kb on - strand, within PP_1449at 1654.893 kb on + strand, within PP_1449at 1654.894 kb on - strand, within PP_1449at 1654.894 kb on - strand, within PP_1449at 1654.894 kb on - strand, within PP_1449at 1654.894 kb on - strand, within PP_1449at 1654.894 kb on - strand, within PP_1449at 1654.954 kb on + strand, within PP_1449at 1655.011 kb on - strand, within PP_1449at 1655.208 kb on + strand, within PP_1449at 1655.356 kb on + strand, within PP_1449at 1655.356 kb on + strand, within PP_1449at 1655.357 kb on - strand, within PP_1449at 1655.459 kb on - strand, within PP_1449at 1655.459 kb on - strand, within PP_1449at 1655.982 kb on + strand, within PP_1449at 1655.986 kb on - strand, within PP_1449at 1655.986 kb on - strand, within PP_1449at 1655.989 kb on + strand, within PP_1449at 1655.989 kb on + strand, within PP_1449at 1655.990 kb on - strand, within PP_1449at 1655.990 kb on - strand, within PP_1449at 1655.990 kb on - strand, within PP_1449at 1656.204 kb on - strand, within PP_1449at 1656.241 kb on - strand, within PP_1449at 1656.249 kb on - strand, within PP_1449at 1656.355 kb on + strandat 1656.355 kb on + strandat 1656.355 kb on + strandat 1656.355 kb on + strandat 1656.355 kb on + strandat 1656.355 kb on + strandat 1656.356 kb on - strandat 1656.356 kb on - strandat 1656.356 kb on - strandat 1656.356 kb on - strandat 1656.360 kb on - strandat 1656.430 kb on - strandat 1656.549 kb on - strandat 1656.749 kb on - strandat 1656.866 kb on + strandat 1656.867 kb on - strandat 1656.867 kb on - strandat 1657.006 kb on + strand, within PP_1450at 1657.006 kb on + strand, within PP_1450at 1657.053 kb on + strand, within PP_1450at 1657.086 kb on - strand, within PP_1450at 1657.086 kb on - strand, within PP_1450at 1657.086 kb on - strand, within PP_1450at 1657.167 kb on - strand, within PP_1450at 1657.244 kb on + strand, within PP_1450at 1657.332 kb on - strand, within PP_1450at 1657.382 kb on - strand, within PP_1450at 1657.382 kb on - strand, within PP_1450at 1657.524 kb on - strand, within PP_1450at 1657.556 kb on + strand, within PP_1450at 1657.574 kb on + strand, within PP_1450at 1657.574 kb on + strand, within PP_1450at 1657.574 kb on + strand, within PP_1450at 1657.575 kb on - strand, within PP_1450at 1657.575 kb on - strand, within PP_1450at 1657.579 kb on + strand, within PP_1450at 1657.579 kb on + strand, within PP_1450at 1657.579 kb on + strand, within PP_1450at 1657.580 kb on - strand, within PP_1450at 1657.580 kb on - strand, within PP_1450at 1657.580 kb on - strand, within PP_1450at 1657.617 kb on - strand, within PP_1450at 1657.628 kb on + strand, within PP_1450at 1657.629 kb on - strand, within PP_1450at 1657.629 kb on - strand, within PP_1450at 1657.629 kb on - strand, within PP_1450at 1657.629 kb on - strand, within PP_1450at 1657.632 kb on - strand, within PP_1450

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM; with MOPS
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1,651,295 + PP_1447 0.40 +0.2
1,651,296 - PP_1447 0.40 -3.2
1,651,296 - PP_1447 0.40 -0.0
1,651,296 - PP_1447 0.40 -1.0
1,651,418 + PP_1447 0.89 -1.4
1,651,418 + PP_1447 0.89 -0.3
1,651,419 - PP_1447 0.90 -0.6
1,651,419 - PP_1447 0.90 -0.7
1,651,520 - PP_1448 0.12 +0.4
1,651,703 + PP_1448 0.40 +0.8
1,651,704 - PP_1448 0.41 +0.7
1,651,725 + PP_1448 0.44 +0.5
1,651,726 - PP_1448 0.44 +0.2
1,651,813 - PP_1448 0.58 -0.2
1,651,813 - PP_1448 0.58 -1.4
1,651,813 - PP_1448 0.58 +0.3
1,651,813 - PP_1448 0.58 +0.3
1,651,821 + PP_1448 0.59 +0.1
1,651,821 + PP_1448 0.59 +0.3
1,651,821 + PP_1448 0.59 +1.1
1,651,822 - PP_1448 0.59 +1.5
1,651,822 - PP_1448 0.59 +1.0
1,651,822 - PP_1448 0.59 -0.4
1,651,822 - PP_1448 0.59 -0.5
1,651,896 + PP_1448 0.71 +2.0
1,651,938 + PP_1448 0.77 -1.4
1,651,938 + PP_1448 0.77 +0.4
1,651,938 + PP_1448 0.77 -0.2
1,651,939 - PP_1448 0.78 +0.4
1,651,939 - PP_1448 0.78 +1.3
1,651,939 - PP_1448 0.78 -0.2
1,651,939 - PP_1448 0.78 +0.5
1,651,975 - PP_1448 0.83 -1.8
1,652,087 - -0.3
1,652,148 - -1.0
1,652,148 - -0.4
1,652,243 + -0.2
1,652,244 - -1.6
1,652,289 + -1.5
1,652,290 - -1.2
1,652,497 + -1.1
1,652,497 + +0.8
1,652,498 - -0.8
1,652,560 - +0.4
1,652,717 + PP_1449 0.10 +0.3
1,652,717 + PP_1449 0.10 -2.4
1,652,717 + PP_1449 0.10 -0.3
1,652,829 + PP_1449 0.13 +0.1
1,652,913 + PP_1449 0.15 +0.7
1,652,913 + PP_1449 0.15 -3.4
1,652,947 + PP_1449 0.16 +1.5
1,653,247 + PP_1449 0.22 +0.9
1,653,247 + PP_1449 0.22 -3.7
1,653,373 + PP_1449 0.25 +0.5
1,653,373 + PP_1449 0.25 +0.1
1,653,373 + PP_1449 0.25 -0.6
1,653,374 - +1.4
1,653,374 - PP_1449 0.25 +0.3
1,653,448 + PP_1449 0.27 -0.5
1,653,449 - PP_1449 0.27 +1.0
1,653,449 - PP_1449 0.27 -0.1
1,653,449 - PP_1449 0.27 +0.3
1,653,521 + PP_1449 0.28 -3.6
1,653,521 + PP_1449 0.28 +0.9
1,653,522 - PP_1449 0.28 -1.7
1,653,696 - PP_1449 0.32 +0.1
1,653,718 + PP_1449 0.33 +1.5
1,653,719 - PP_1449 0.33 -0.1
1,653,740 + PP_1449 0.33 -1.7
1,653,741 - PP_1449 0.33 +0.5
1,653,860 + PP_1449 0.36 +0.7
1,653,860 + PP_1449 0.36 -0.2
1,653,861 - PP_1449 0.36 -0.0
1,653,861 - PP_1449 0.36 +0.7
1,653,861 - PP_1449 0.36 +2.7
1,654,188 - PP_1449 0.43 -0.3
1,654,209 + PP_1449 0.43 -0.2
1,654,504 + PP_1449 0.50 +0.5
1,654,505 - PP_1449 0.50 +0.4
1,654,505 - PP_1449 0.50 -2.4
1,654,698 - PP_1449 0.54 +0.1
1,654,893 + PP_1449 0.59 -1.4
1,654,894 - PP_1449 0.59 +1.0
1,654,894 - PP_1449 0.59 +0.9
1,654,894 - PP_1449 0.59 -0.1
1,654,894 - PP_1449 0.59 +0.9
1,654,894 - PP_1449 0.59 -0.1
1,654,954 + PP_1449 0.60 -0.1
1,655,011 - PP_1449 0.61 -1.2
1,655,208 + PP_1449 0.66 +0.4
1,655,356 + PP_1449 0.69 +1.4
1,655,356 + PP_1449 0.69 +0.4
1,655,357 - PP_1449 0.69 +0.2
1,655,459 - PP_1449 0.71 -0.1
1,655,459 - PP_1449 0.71 -1.0
1,655,982 + PP_1449 0.83 +1.2
1,655,986 - PP_1449 0.83 -0.1
1,655,986 - PP_1449 0.83 +0.3
1,655,989 + PP_1449 0.83 +1.6
1,655,989 + PP_1449 0.83 -0.1
1,655,990 - PP_1449 0.83 +0.1
1,655,990 - PP_1449 0.83 -3.3
1,655,990 - PP_1449 0.83 -1.4
1,656,204 - PP_1449 0.88 +0.9
1,656,241 - PP_1449 0.88 -1.6
1,656,249 - PP_1449 0.89 -0.2
1,656,355 + -0.0
1,656,355 + +0.5
1,656,355 + -0.2
1,656,355 + +1.6
1,656,355 + +0.9
1,656,355 + +0.1
1,656,356 - +0.1
1,656,356 - -0.3
1,656,356 - -4.0
1,656,356 - +0.7
1,656,360 - +1.0
1,656,430 - +1.0
1,656,549 - -0.7
1,656,749 - +0.1
1,656,866 + -0.1
1,656,867 - +1.0
1,656,867 - -2.7
1,657,006 + PP_1450 0.12 -1.1
1,657,006 + PP_1450 0.12 -0.2
1,657,053 + PP_1450 0.15 -0.3
1,657,086 - PP_1450 0.17 -0.7
1,657,086 - PP_1450 0.17 +0.3
1,657,086 - PP_1450 0.17 -1.0
1,657,167 - PP_1450 0.22 +0.8
1,657,244 + PP_1450 0.26 +0.3
1,657,332 - PP_1450 0.32 -0.3
1,657,382 - PP_1450 0.35 +0.2
1,657,382 - PP_1450 0.35 -0.4
1,657,524 - PP_1450 0.43 -1.9
1,657,556 + PP_1450 0.45 +0.8
1,657,574 + PP_1450 0.46 +1.9
1,657,574 + PP_1450 0.46 -0.9
1,657,574 + PP_1450 0.46 +0.2
1,657,575 - PP_1450 0.46 -0.9
1,657,575 - PP_1450 0.46 +0.4
1,657,579 + PP_1450 0.46 +1.0
1,657,579 + PP_1450 0.46 +0.9
1,657,579 + PP_1450 0.46 -0.1
1,657,580 - PP_1450 0.46 -1.5
1,657,580 - PP_1450 0.46 -0.6
1,657,580 - PP_1450 0.46 -0.8
1,657,617 - PP_1450 0.48 -1.7
1,657,628 + PP_1450 0.49 +0.4
1,657,629 - PP_1450 0.49 -1.1
1,657,629 - PP_1450 0.49 +1.5
1,657,629 - PP_1450 0.49 +0.1
1,657,629 - PP_1450 0.49 -0.1
1,657,632 - PP_1450 0.49 -0.3

Or see this region's nucleotide sequence