Experiment: N4_phage 0.00009375 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atoS and atoC overlap by 4 nucleotides atoC and atoD are separated by 195 nucleotides atoD and atoA overlap by 1 nucleotides
b2219: atoS - sensory histidine kinase in two-component regulatory system with AtoC (NCBI), at 2,318,065 to 2,319,891
atoS
b2220: atoC - fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI), at 2,319,888 to 2,321,273
atoC
b2221: atoD - acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI), at 2,321,469 to 2,322,131
atoD
b2222: atoA - acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (NCBI), at 2,322,131 to 2,322,781
atoA
Position (kb)
2319
2320
2321
2322 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2318.911 kb on + strand, within atoS at 2318.960 kb on + strand, within atoS at 2318.960 kb on + strand, within atoS at 2318.979 kb on + strand, within atoS at 2318.979 kb on + strand, within atoS at 2318.987 kb on - strand, within atoS at 2319.017 kb on - strand, within atoS at 2319.017 kb on - strand, within atoS at 2319.094 kb on + strand, within atoS at 2319.102 kb on + strand, within atoS at 2319.125 kb on + strand, within atoS at 2319.212 kb on - strand, within atoS at 2319.238 kb on - strand, within atoS at 2319.270 kb on - strand, within atoS at 2319.296 kb on - strand, within atoS at 2319.323 kb on + strand, within atoS at 2319.323 kb on + strand, within atoS at 2319.394 kb on - strand, within atoS at 2319.394 kb on - strand, within atoS at 2319.415 kb on + strand, within atoS at 2319.415 kb on + strand, within atoS at 2319.461 kb on - strand, within atoS at 2319.461 kb on - strand, within atoS at 2319.466 kb on + strand, within atoS at 2319.483 kb on - strand, within atoS at 2319.551 kb on + strand, within atoS at 2319.559 kb on - strand, within atoS at 2319.559 kb on - strand, within atoS at 2319.574 kb on + strand, within atoS at 2319.598 kb on + strand, within atoS at 2319.598 kb on + strand, within atoS at 2319.632 kb on + strand, within atoS at 2319.640 kb on + strand, within atoS at 2319.648 kb on - strand, within atoS at 2319.653 kb on + strand, within atoS at 2319.653 kb on + strand, within atoS at 2319.653 kb on + strand, within atoS at 2319.661 kb on - strand, within atoS at 2319.661 kb on - strand, within atoS at 2319.787 kb on + strand at 2319.920 kb on - strand at 2319.934 kb on - strand at 2319.965 kb on + strand at 2319.965 kb on + strand at 2320.005 kb on + strand at 2320.013 kb on - strand at 2320.013 kb on - strand at 2320.045 kb on + strand, within atoC at 2320.051 kb on - strand, within atoC at 2320.102 kb on + strand, within atoC at 2320.104 kb on + strand, within atoC at 2320.146 kb on + strand, within atoC at 2320.146 kb on + strand, within atoC at 2320.240 kb on + strand, within atoC at 2320.251 kb on - strand, within atoC at 2320.251 kb on - strand, within atoC at 2320.298 kb on - strand, within atoC at 2320.600 kb on + strand, within atoC at 2320.640 kb on + strand, within atoC at 2320.641 kb on + strand, within atoC at 2320.668 kb on + strand, within atoC at 2320.668 kb on + strand, within atoC at 2320.671 kb on - strand, within atoC at 2320.674 kb on - strand, within atoC at 2320.752 kb on + strand, within atoC at 2320.752 kb on + strand, within atoC at 2320.833 kb on - strand, within atoC at 2320.833 kb on - strand, within atoC at 2320.936 kb on + strand, within atoC at 2320.936 kb on + strand, within atoC at 2320.938 kb on + strand, within atoC at 2320.944 kb on - strand, within atoC at 2320.944 kb on - strand, within atoC at 2320.972 kb on + strand, within atoC at 2320.972 kb on + strand, within atoC at 2321.007 kb on + strand, within atoC at 2321.014 kb on + strand, within atoC at 2321.179 kb on - strand at 2321.194 kb on - strand at 2321.196 kb on + strand at 2321.196 kb on + strand at 2321.236 kb on - strand at 2321.261 kb on + strand at 2321.266 kb on + strand at 2321.496 kb on - strand at 2321.519 kb on - strand at 2321.700 kb on + strand, within atoD at 2321.707 kb on - strand, within atoD at 2321.818 kb on + strand, within atoD at 2321.967 kb on + strand, within atoD at 2322.085 kb on - strand at 2322.255 kb on - strand, within atoA
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC1 remove 2,318,911 + atoS b2219 0.46 -0.4 2,318,960 + atoS b2219 0.49 +0.3 2,318,960 + atoS b2219 0.49 +1.2 2,318,979 + atoS b2219 0.50 +0.0 2,318,979 + atoS b2219 0.50 -0.9 2,318,987 - atoS b2219 0.50 -0.6 2,319,017 - atoS b2219 0.52 +0.3 2,319,017 - atoS b2219 0.52 +0.1 2,319,094 + atoS b2219 0.56 +0.3 2,319,102 + atoS b2219 0.57 +0.0 2,319,125 + atoS b2219 0.58 -0.4 2,319,212 - atoS b2219 0.63 -0.6 2,319,238 - atoS b2219 0.64 -0.4 2,319,270 - atoS b2219 0.66 -0.1 2,319,296 - atoS b2219 0.67 +1.2 2,319,323 + atoS b2219 0.69 -0.2 2,319,323 + atoS b2219 0.69 -2.1 2,319,394 - atoS b2219 0.73 -0.4 2,319,394 - atoS b2219 0.73 +0.3 2,319,415 + atoS b2219 0.74 -1.0 2,319,415 + atoS b2219 0.74 -1.2 2,319,461 - atoS b2219 0.76 -3.6 2,319,461 - atoS b2219 0.76 +0.2 2,319,466 + atoS b2219 0.77 -0.3 2,319,483 - atoS b2219 0.78 +0.5 2,319,551 + atoS b2219 0.81 -0.5 2,319,559 - atoS b2219 0.82 +0.8 2,319,559 - atoS b2219 0.82 +0.0 2,319,574 + atoS b2219 0.83 -0.6 2,319,598 + atoS b2219 0.84 +1.8 2,319,598 + atoS b2219 0.84 +0.4 2,319,632 + atoS b2219 0.86 +0.2 2,319,640 + atoS b2219 0.86 -0.5 2,319,648 - atoS b2219 0.87 -0.2 2,319,653 + atoS b2219 0.87 +0.9 2,319,653 + atoS b2219 0.87 +1.0 2,319,653 + atoS b2219 0.87 -1.1 2,319,661 - atoS b2219 0.87 -0.4 2,319,661 - atoS b2219 0.87 +0.1 2,319,787 + +0.1 2,319,920 - -1.8 2,319,934 - -1.7 2,319,965 + -1.1 2,319,965 + -0.3 2,320,005 + -0.7 2,320,013 - -0.7 2,320,013 - -0.6 2,320,045 + atoC b2220 0.11 -0.6 2,320,051 - atoC b2220 0.12 +0.2 2,320,102 + atoC b2220 0.15 -0.1 2,320,104 + atoC b2220 0.16 +0.3 2,320,146 + atoC b2220 0.19 -0.4 2,320,146 + atoC b2220 0.19 -0.5 2,320,240 + atoC b2220 0.25 -0.1 2,320,251 - atoC b2220 0.26 +0.4 2,320,251 - atoC b2220 0.26 +0.6 2,320,298 - atoC b2220 0.30 -1.4 2,320,600 + atoC b2220 0.51 +1.0 2,320,640 + atoC b2220 0.54 +1.5 2,320,641 + atoC b2220 0.54 +0.1 2,320,668 + atoC b2220 0.56 -2.3 2,320,668 + atoC b2220 0.56 -0.5 2,320,671 - atoC b2220 0.56 +0.9 2,320,674 - atoC b2220 0.57 +1.2 2,320,752 + atoC b2220 0.62 -1.6 2,320,752 + atoC b2220 0.62 -0.3 2,320,833 - atoC b2220 0.68 +1.0 2,320,833 - atoC b2220 0.68 -1.1 2,320,936 + atoC b2220 0.76 -0.5 2,320,936 + atoC b2220 0.76 -0.3 2,320,938 + atoC b2220 0.76 -0.7 2,320,944 - atoC b2220 0.76 +1.0 2,320,944 - atoC b2220 0.76 -0.9 2,320,972 + atoC b2220 0.78 -0.1 2,320,972 + atoC b2220 0.78 -0.0 2,321,007 + atoC b2220 0.81 -0.7 2,321,014 + atoC b2220 0.81 -0.2 2,321,179 - +0.1 2,321,194 - -1.6 2,321,196 + -1.0 2,321,196 + +0.1 2,321,236 - -0.5 2,321,261 + -1.0 2,321,266 + +0.1 2,321,496 - -0.6 2,321,519 - -0.3 2,321,700 + atoD b2221 0.35 -1.4 2,321,707 - atoD b2221 0.36 -0.6 2,321,818 + atoD b2221 0.53 +0.6 2,321,967 + atoD b2221 0.75 -0.0 2,322,085 - +0.2 2,322,255 - atoA b2222 0.19 -0.4
Or see this region's nucleotide sequence