Experiment: N4_phage 0.00009375 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcsC and atoS are separated by 166 nucleotides atoS and atoC overlap by 4 nucleotides
b2218: rcsC - hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq), at 2,315,049 to 2,317,898
rcsC
b2219: atoS - sensory histidine kinase in two-component regulatory system with AtoC (NCBI), at 2,318,065 to 2,319,891
atoS
b2220: atoC - fused response regulator of ato operon, in two-component system with AtoS: response regulator/sigma54 interaction protein (NCBI), at 2,319,888 to 2,321,273
atoC
Position (kb)
2318
2319
2320 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2317.181 kb on + strand, within rcsC at 2317.315 kb on + strand, within rcsC at 2317.395 kb on + strand, within rcsC at 2317.416 kb on - strand, within rcsC at 2317.538 kb on - strand, within rcsC at 2317.589 kb on + strand, within rcsC at 2317.635 kb on - strand at 2317.682 kb on + strand at 2317.818 kb on - strand at 2317.822 kb on - strand at 2317.931 kb on + strand at 2317.936 kb on + strand at 2318.000 kb on - strand at 2318.000 kb on - strand at 2318.067 kb on + strand at 2318.114 kb on + strand at 2318.128 kb on - strand at 2318.163 kb on - strand at 2318.189 kb on - strand at 2318.244 kb on + strand at 2318.265 kb on - strand, within atoS at 2318.371 kb on - strand, within atoS at 2318.461 kb on - strand, within atoS at 2318.462 kb on - strand, within atoS at 2318.473 kb on - strand, within atoS at 2318.473 kb on - strand, within atoS at 2318.473 kb on - strand, within atoS at 2318.514 kb on + strand, within atoS at 2318.514 kb on + strand, within atoS at 2318.558 kb on + strand, within atoS at 2318.558 kb on + strand, within atoS at 2318.610 kb on + strand, within atoS at 2318.664 kb on + strand, within atoS at 2318.672 kb on - strand, within atoS at 2318.810 kb on + strand, within atoS at 2318.827 kb on - strand, within atoS at 2318.911 kb on + strand, within atoS at 2318.960 kb on + strand, within atoS at 2318.960 kb on + strand, within atoS at 2318.979 kb on + strand, within atoS at 2318.979 kb on + strand, within atoS at 2318.987 kb on - strand, within atoS at 2319.017 kb on - strand, within atoS at 2319.017 kb on - strand, within atoS at 2319.094 kb on + strand, within atoS at 2319.102 kb on + strand, within atoS at 2319.125 kb on + strand, within atoS at 2319.212 kb on - strand, within atoS at 2319.238 kb on - strand, within atoS at 2319.270 kb on - strand, within atoS at 2319.296 kb on - strand, within atoS at 2319.323 kb on + strand, within atoS at 2319.323 kb on + strand, within atoS at 2319.394 kb on - strand, within atoS at 2319.394 kb on - strand, within atoS at 2319.415 kb on + strand, within atoS at 2319.415 kb on + strand, within atoS at 2319.461 kb on - strand, within atoS at 2319.461 kb on - strand, within atoS at 2319.466 kb on + strand, within atoS at 2319.483 kb on - strand, within atoS at 2319.551 kb on + strand, within atoS at 2319.559 kb on - strand, within atoS at 2319.559 kb on - strand, within atoS at 2319.574 kb on + strand, within atoS at 2319.598 kb on + strand, within atoS at 2319.598 kb on + strand, within atoS at 2319.632 kb on + strand, within atoS at 2319.640 kb on + strand, within atoS at 2319.648 kb on - strand, within atoS at 2319.653 kb on + strand, within atoS at 2319.653 kb on + strand, within atoS at 2319.653 kb on + strand, within atoS at 2319.661 kb on - strand, within atoS at 2319.661 kb on - strand, within atoS at 2319.787 kb on + strand at 2319.920 kb on - strand at 2319.934 kb on - strand at 2319.965 kb on + strand at 2319.965 kb on + strand at 2320.005 kb on + strand at 2320.013 kb on - strand at 2320.013 kb on - strand at 2320.045 kb on + strand, within atoC at 2320.051 kb on - strand, within atoC at 2320.102 kb on + strand, within atoC at 2320.104 kb on + strand, within atoC at 2320.146 kb on + strand, within atoC at 2320.146 kb on + strand, within atoC at 2320.240 kb on + strand, within atoC at 2320.251 kb on - strand, within atoC at 2320.251 kb on - strand, within atoC at 2320.298 kb on - strand, within atoC at 2320.600 kb on + strand, within atoC at 2320.640 kb on + strand, within atoC at 2320.641 kb on + strand, within atoC at 2320.668 kb on + strand, within atoC at 2320.668 kb on + strand, within atoC at 2320.671 kb on - strand, within atoC at 2320.674 kb on - strand, within atoC at 2320.752 kb on + strand, within atoC at 2320.752 kb on + strand, within atoC at 2320.833 kb on - strand, within atoC at 2320.833 kb on - strand, within atoC
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC1 remove 2,317,181 + rcsC b2218 0.75 +0.3 2,317,315 + rcsC b2218 0.80 -1.1 2,317,395 + rcsC b2218 0.82 -0.3 2,317,416 - rcsC b2218 0.83 -0.2 2,317,538 - rcsC b2218 0.87 +0.2 2,317,589 + rcsC b2218 0.89 -1.7 2,317,635 - -0.3 2,317,682 + -0.2 2,317,818 - +0.3 2,317,822 - +0.3 2,317,931 + -1.1 2,317,936 + -1.6 2,318,000 - +0.4 2,318,000 - +0.8 2,318,067 + +0.0 2,318,114 + -0.1 2,318,128 - -0.2 2,318,163 - -0.6 2,318,189 - -0.1 2,318,244 + +0.5 2,318,265 - atoS b2219 0.11 -3.2 2,318,371 - atoS b2219 0.17 +1.1 2,318,461 - atoS b2219 0.22 -1.9 2,318,462 - atoS b2219 0.22 -0.0 2,318,473 - atoS b2219 0.22 -0.1 2,318,473 - atoS b2219 0.22 -2.2 2,318,473 - atoS b2219 0.22 +0.5 2,318,514 + atoS b2219 0.25 -0.6 2,318,514 + atoS b2219 0.25 +1.1 2,318,558 + atoS b2219 0.27 -0.0 2,318,558 + atoS b2219 0.27 -1.2 2,318,610 + atoS b2219 0.30 +1.2 2,318,664 + atoS b2219 0.33 -0.1 2,318,672 - atoS b2219 0.33 -0.3 2,318,810 + atoS b2219 0.41 -0.7 2,318,827 - atoS b2219 0.42 -1.5 2,318,911 + atoS b2219 0.46 -0.4 2,318,960 + atoS b2219 0.49 +0.3 2,318,960 + atoS b2219 0.49 +1.2 2,318,979 + atoS b2219 0.50 +0.0 2,318,979 + atoS b2219 0.50 -0.9 2,318,987 - atoS b2219 0.50 -0.6 2,319,017 - atoS b2219 0.52 +0.3 2,319,017 - atoS b2219 0.52 +0.1 2,319,094 + atoS b2219 0.56 +0.3 2,319,102 + atoS b2219 0.57 +0.0 2,319,125 + atoS b2219 0.58 -0.4 2,319,212 - atoS b2219 0.63 -0.6 2,319,238 - atoS b2219 0.64 -0.4 2,319,270 - atoS b2219 0.66 -0.1 2,319,296 - atoS b2219 0.67 +1.2 2,319,323 + atoS b2219 0.69 -0.2 2,319,323 + atoS b2219 0.69 -2.1 2,319,394 - atoS b2219 0.73 -0.4 2,319,394 - atoS b2219 0.73 +0.3 2,319,415 + atoS b2219 0.74 -1.0 2,319,415 + atoS b2219 0.74 -1.2 2,319,461 - atoS b2219 0.76 -3.6 2,319,461 - atoS b2219 0.76 +0.2 2,319,466 + atoS b2219 0.77 -0.3 2,319,483 - atoS b2219 0.78 +0.5 2,319,551 + atoS b2219 0.81 -0.5 2,319,559 - atoS b2219 0.82 +0.8 2,319,559 - atoS b2219 0.82 +0.0 2,319,574 + atoS b2219 0.83 -0.6 2,319,598 + atoS b2219 0.84 +1.8 2,319,598 + atoS b2219 0.84 +0.4 2,319,632 + atoS b2219 0.86 +0.2 2,319,640 + atoS b2219 0.86 -0.5 2,319,648 - atoS b2219 0.87 -0.2 2,319,653 + atoS b2219 0.87 +0.9 2,319,653 + atoS b2219 0.87 +1.0 2,319,653 + atoS b2219 0.87 -1.1 2,319,661 - atoS b2219 0.87 -0.4 2,319,661 - atoS b2219 0.87 +0.1 2,319,787 + +0.1 2,319,920 - -1.8 2,319,934 - -1.7 2,319,965 + -1.1 2,319,965 + -0.3 2,320,005 + -0.7 2,320,013 - -0.7 2,320,013 - -0.6 2,320,045 + atoC b2220 0.11 -0.6 2,320,051 - atoC b2220 0.12 +0.2 2,320,102 + atoC b2220 0.15 -0.1 2,320,104 + atoC b2220 0.16 +0.3 2,320,146 + atoC b2220 0.19 -0.4 2,320,146 + atoC b2220 0.19 -0.5 2,320,240 + atoC b2220 0.25 -0.1 2,320,251 - atoC b2220 0.26 +0.4 2,320,251 - atoC b2220 0.26 +0.6 2,320,298 - atoC b2220 0.30 -1.4 2,320,600 + atoC b2220 0.51 +1.0 2,320,640 + atoC b2220 0.54 +1.5 2,320,641 + atoC b2220 0.54 +0.1 2,320,668 + atoC b2220 0.56 -2.3 2,320,668 + atoC b2220 0.56 -0.5 2,320,671 - atoC b2220 0.56 +0.9 2,320,674 - atoC b2220 0.57 +1.2 2,320,752 + atoC b2220 0.62 -1.6 2,320,752 + atoC b2220 0.62 -0.3 2,320,833 - atoC b2220 0.68 +1.0 2,320,833 - atoC b2220 0.68 -1.1
Or see this region's nucleotide sequence