Experiment: N4_phage 0.00009375 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folM and ydgC overlap by 4 nucleotides ydgC and rstA are separated by 128 nucleotides rstA and rstB are separated by 3 nucleotides
b1606: folM - short chain dehydrogenase (NCBI), at 1,679,000 to 1,679,722
folM
b1607: ydgC - conserved inner membrane protein associated with alginate biosynthesis (NCBI), at 1,679,719 to 1,680,054
ydgC
b1608: rstA - DNA-binding response regulator in two-component regulatory system with RstB (RefSeq), at 1,680,183 to 1,680,902
rstA
b1609: rstB - sensory histidine kinase in two-component regulatory system with RstA (NCBI), at 1,680,906 to 1,682,207
rstB
Position (kb)
1680
1681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1679.198 kb on - strand, within folM at 1679.226 kb on + strand, within folM at 1679.328 kb on - strand, within folM at 1679.336 kb on - strand, within folM at 1679.342 kb on + strand, within folM at 1679.342 kb on - strand, within folM at 1679.342 kb on - strand, within folM at 1679.342 kb on - strand, within folM at 1679.349 kb on + strand, within folM at 1679.376 kb on + strand, within folM at 1679.390 kb on + strand, within folM at 1679.484 kb on - strand, within folM at 1679.538 kb on + strand, within folM at 1679.538 kb on + strand, within folM at 1679.887 kb on + strand, within ydgC at 1679.897 kb on + strand, within ydgC at 1679.924 kb on - strand, within ydgC at 1680.147 kb on + strand at 1680.268 kb on + strand, within rstA at 1680.438 kb on + strand, within rstA at 1680.439 kb on + strand, within rstA at 1680.439 kb on + strand, within rstA at 1680.544 kb on + strand, within rstA at 1680.552 kb on - strand, within rstA at 1680.552 kb on - strand, within rstA at 1680.575 kb on + strand, within rstA at 1680.981 kb on + strand at 1680.981 kb on + strand at 1681.080 kb on + strand, within rstB at 1681.099 kb on - strand, within rstB at 1681.176 kb on + strand, within rstB at 1681.555 kb on + strand, within rstB at 1681.555 kb on + strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.779 kb on - strand, within rstB at 1681.827 kb on + strand, within rstB
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC1 remove 1,679,198 - folM b1606 0.27 -0.2 1,679,226 + folM b1606 0.31 -2.0 1,679,328 - folM b1606 0.45 +0.2 1,679,336 - folM b1606 0.46 +2.4 1,679,342 + folM b1606 0.47 +1.0 1,679,342 - folM b1606 0.47 +0.2 1,679,342 - folM b1606 0.47 +0.3 1,679,342 - folM b1606 0.47 +0.1 1,679,349 + folM b1606 0.48 -0.4 1,679,376 + folM b1606 0.52 -1.7 1,679,390 + folM b1606 0.54 +0.1 1,679,484 - folM b1606 0.67 +0.5 1,679,538 + folM b1606 0.74 -0.2 1,679,538 + folM b1606 0.74 -2.0 1,679,887 + ydgC b1607 0.50 +1.3 1,679,897 + ydgC b1607 0.53 +1.3 1,679,924 - ydgC b1607 0.61 +0.5 1,680,147 + -0.1 1,680,268 + rstA b1608 0.12 +0.6 1,680,438 + rstA b1608 0.35 +2.5 1,680,439 + rstA b1608 0.36 -1.5 1,680,439 + rstA b1608 0.36 -0.9 1,680,544 + rstA b1608 0.50 -0.3 1,680,552 - rstA b1608 0.51 +1.9 1,680,552 - rstA b1608 0.51 -0.5 1,680,575 + rstA b1608 0.54 -0.2 1,680,981 + -1.3 1,680,981 + -0.0 1,681,080 + rstB b1609 0.13 -0.2 1,681,099 - rstB b1609 0.15 +0.5 1,681,176 + rstB b1609 0.21 -2.4 1,681,555 + rstB b1609 0.50 +0.6 1,681,555 + rstB b1609 0.50 -1.2 1,681,779 - rstB b1609 0.67 -1.0 1,681,779 - rstB b1609 0.67 -1.0 1,681,827 + rstB b1609 0.71 +0.8
Or see this region's nucleotide sequence