Strain Fitness in Escherichia coli BW25113 around b1462

Experiment: N4_phage 0.00009375 MOI TC1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N4_phage 0.00009375 MOI TC1
1,531,010	+				+2.3
1,531,025	+				+2.7
1,531,035	-				-0.3
1,531,066	-				+1.3
1,531,128	+	ydcE	b1461	0.22	-0.3
1,531,128	+	ydcE	b1461	0.22	+1.6
1,531,177	+	ydcE	b1461	0.43	+0.3
1,531,197	+	ydcE	b1461	0.52	+2.3
1,531,197	+	ydcE	b1461	0.52	+1.3
1,531,210	-	ydcE	b1461	0.57	-0.3
1,531,211	-	ydcE	b1461	0.58	+0.3
1,531,215	-	ydcE	b1461	0.59	+0.4
1,531,233	-	ydcE	b1461	0.67	-0.5
1,531,238	+	ydcE	b1461	0.69	+0.0
1,531,238	+				+0.4
1,531,381	-	b1462	b1462	0.13	-0.3
1,531,599	+	b1462	b1462	0.51	-1.4
1,531,972	-				-2.5
1,531,972	-				+2.1
1,532,030	-				+0.9
1,532,030	-				-2.0
1,532,151	-	nhoA	b1463	0.12	+1.4
1,532,151	-	nhoA	b1463	0.12	+0.2
1,532,151	-	nhoA	b1463	0.12	+0.3
1,532,159	+	nhoA	b1463	0.13	+1.9
1,532,159	+	nhoA	b1463	0.13	+0.7
1,532,497	-	nhoA	b1463	0.53	+0.5
1,532,497	-	nhoA	b1463	0.53	+0.0
1,532,501	+	nhoA	b1463	0.54	+1.1
1,532,501	+	nhoA	b1463	0.54	-0.8
1,532,517	-	nhoA	b1463	0.55	-0.5
1,532,530	-	nhoA	b1463	0.57	+0.9
1,532,595	+	nhoA	b1463	0.65	-0.1
1,532,688	+	nhoA	b1463	0.76	-0.7
1,532,696	-	nhoA	b1463	0.77	-0.3
1,532,746	+	nhoA	b1463	0.83	-1.7
1,532,785	-	nhoA	b1463	0.87	-2.5
1,532,834	-				-0.3