Experiment: N4_phage 0.00009375 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfrA and nfrB overlap by 14 nucleotides nfrB and cusS are separated by 149 nucleotides
b0568: nfrA - bacteriophage N4 receptor, outer membrane subunit (NCBI), at 587,205 to 590,177
nfrA
b0569: nfrB - bacteriophage N4 receptor, inner membrane subunit (NCBI), at 590,164 to 592,401
nfrB
b0570: cusS - sensory histidine kinase in two-component regulatory system with CusR, senses copper ions (NCBI), at 592,551 to 593,993
cusS
Position (kb) 590
591
592
593 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 589.179 kb on + strand, within nfrA at 589.200 kb on - strand, within nfrA at 589.255 kb on - strand, within nfrA at 589.452 kb on + strand, within nfrA at 589.523 kb on + strand, within nfrA at 589.546 kb on + strand, within nfrA at 589.546 kb on + strand, within nfrA at 589.656 kb on + strand, within nfrA at 589.780 kb on + strand, within nfrA at 589.789 kb on - strand, within nfrA at 589.829 kb on - strand, within nfrA at 589.857 kb on - strand, within nfrA at 589.908 kb on + strand at 589.908 kb on + strand at 589.919 kb on + strand at 589.928 kb on - strand at 589.928 kb on - strand at 589.961 kb on - strand at 589.964 kb on + strand at 589.977 kb on + strand at 590.006 kb on - strand at 590.010 kb on + strand at 590.010 kb on + strand at 590.071 kb on - strand at 590.071 kb on - strand at 590.071 kb on - strand at 590.088 kb on + strand at 590.099 kb on + strand at 590.142 kb on - strand at 590.143 kb on - strand at 590.163 kb on - strand at 590.163 kb on - strand at 590.195 kb on - strand at 590.195 kb on - strand at 590.202 kb on + strand at 590.202 kb on + strand at 590.217 kb on - strand at 590.232 kb on - strand at 590.247 kb on + strand at 590.309 kb on - strand at 590.344 kb on - strand at 590.344 kb on - strand at 590.438 kb on - strand, within nfrB at 590.474 kb on + strand, within nfrB at 590.482 kb on - strand, within nfrB at 590.789 kb on + strand, within nfrB at 590.789 kb on + strand, within nfrB at 590.872 kb on + strand, within nfrB at 590.872 kb on + strand, within nfrB at 590.885 kb on - strand, within nfrB at 590.885 kb on - strand, within nfrB at 591.089 kb on + strand, within nfrB at 591.089 kb on + strand, within nfrB at 591.188 kb on + strand, within nfrB at 591.188 kb on + strand, within nfrB at 591.188 kb on + strand, within nfrB at 591.189 kb on - strand, within nfrB at 591.257 kb on + strand, within nfrB at 591.257 kb on + strand, within nfrB at 591.318 kb on - strand, within nfrB at 591.318 kb on - strand, within nfrB at 591.343 kb on - strand, within nfrB at 591.343 kb on - strand, within nfrB at 591.705 kb on + strand, within nfrB at 591.750 kb on - strand, within nfrB at 591.750 kb on - strand, within nfrB at 591.767 kb on + strand, within nfrB at 591.808 kb on + strand, within nfrB at 591.888 kb on + strand, within nfrB at 592.009 kb on + strand, within nfrB at 592.011 kb on + strand, within nfrB at 592.021 kb on - strand, within nfrB at 592.025 kb on + strand, within nfrB at 592.168 kb on - strand, within nfrB at 592.179 kb on - strand at 592.205 kb on + strand at 592.205 kb on + strand at 592.565 kb on - strand at 592.565 kb on - strand at 592.696 kb on + strand, within cusS at 592.776 kb on + strand, within cusS at 592.776 kb on + strand, within cusS at 592.809 kb on - strand, within cusS at 592.822 kb on + strand, within cusS at 592.828 kb on - strand, within cusS at 592.828 kb on - strand, within cusS at 593.025 kb on - strand, within cusS at 593.031 kb on - strand, within cusS at 593.083 kb on + strand, within cusS at 593.091 kb on + strand, within cusS at 593.091 kb on + strand, within cusS at 593.248 kb on - strand, within cusS at 593.368 kb on + strand, within cusS
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.00009375 MOI TC1 remove 589,179 + nfrA b0568 0.66 +2.6 589,200 - nfrA b0568 0.67 +3.3 589,255 - nfrA b0568 0.69 +3.6 589,452 + nfrA b0568 0.76 +2.6 589,523 + nfrA b0568 0.78 +3.3 589,546 + nfrA b0568 0.79 +2.8 589,546 + nfrA b0568 0.79 +3.2 589,656 + nfrA b0568 0.82 +3.3 589,780 + nfrA b0568 0.87 +3.6 589,789 - nfrA b0568 0.87 +3.7 589,829 - nfrA b0568 0.88 +3.4 589,857 - nfrA b0568 0.89 +3.7 589,908 + +3.7 589,908 + +3.6 589,919 + +4.0 589,928 - +3.6 589,928 - +4.3 589,961 - +3.0 589,964 + +3.4 589,977 + +3.5 590,006 - +3.4 590,010 + +3.1 590,010 + +4.1 590,071 - +3.6 590,071 - +3.5 590,071 - +3.7 590,088 + +3.6 590,099 + +3.6 590,142 - +3.1 590,143 - +3.5 590,163 - +1.2 590,163 - +0.1 590,195 - +0.4 590,195 - +0.5 590,202 + +2.0 590,202 + +2.6 590,217 - +0.1 590,232 - +2.8 590,247 + +2.7 590,309 - +4.9 590,344 - +2.8 590,344 - +2.6 590,438 - nfrB b0569 0.12 +3.4 590,474 + nfrB b0569 0.14 +3.3 590,482 - nfrB b0569 0.14 +3.0 590,789 + nfrB b0569 0.28 +3.7 590,789 + nfrB b0569 0.28 +2.2 590,872 + nfrB b0569 0.32 +3.3 590,872 + nfrB b0569 0.32 +3.1 590,885 - nfrB b0569 0.32 +3.4 590,885 - nfrB b0569 0.32 +3.6 591,089 + nfrB b0569 0.41 +3.6 591,089 + nfrB b0569 0.41 +3.4 591,188 + nfrB b0569 0.46 +3.7 591,188 + nfrB b0569 0.46 +3.4 591,188 + nfrB b0569 0.46 +3.8 591,189 - nfrB b0569 0.46 +3.4 591,257 + nfrB b0569 0.49 +3.0 591,257 + nfrB b0569 0.49 +3.6 591,318 - nfrB b0569 0.52 +3.4 591,318 - nfrB b0569 0.52 +3.5 591,343 - nfrB b0569 0.53 +3.5 591,343 - nfrB b0569 0.53 +4.0 591,705 + nfrB b0569 0.69 +3.5 591,750 - nfrB b0569 0.71 +3.7 591,750 - nfrB b0569 0.71 +3.6 591,767 + nfrB b0569 0.72 +3.2 591,808 + nfrB b0569 0.73 +3.5 591,888 + nfrB b0569 0.77 +3.6 592,009 + nfrB b0569 0.82 +3.6 592,011 + nfrB b0569 0.83 +3.2 592,021 - nfrB b0569 0.83 +4.4 592,025 + nfrB b0569 0.83 +3.2 592,168 - nfrB b0569 0.90 +3.0 592,179 - +3.0 592,205 + +2.9 592,205 + +4.2 592,565 - +1.7 592,565 - +0.2 592,696 + cusS b0570 0.10 +0.4 592,776 + cusS b0570 0.16 -0.3 592,776 + cusS b0570 0.16 +0.7 592,809 - cusS b0570 0.18 -0.9 592,822 + cusS b0570 0.19 -0.4 592,828 - cusS b0570 0.19 +0.3 592,828 - cusS b0570 0.19 -0.9 593,025 - cusS b0570 0.33 -0.5 593,031 - cusS b0570 0.33 +0.4 593,083 + cusS b0570 0.37 -0.8 593,091 + cusS b0570 0.37 -0.5 593,091 + cusS b0570 0.37 -0.5 593,248 - cusS b0570 0.48 +0.6 593,368 + cusS b0570 0.57 -0.3
Or see this region's nucleotide sequence