Strain Fitness in Escherichia coli BW25113 around b0294

Experiment: N4_phage 0.00009375 MOI TC1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	N4_phage 0.00009375 MOI TC1
308,984	+	yagY	b0292	0.60	-1.5
309,053	-	yagY	b0292	0.70	-0.3
309,149	+	yagY	b0292	0.85	-0.4
309,158	-	yagY	b0292	0.86	-0.6
309,166	-	yagY	b0292	0.87	+0.6
309,300	-				-0.1
309,312	-				-0.2
309,370	-	yagZ	b0293	0.11	-0.3
309,370	-	yagZ	b0293	0.11	+0.2
309,401	-	yagZ	b0293	0.16	-0.1
309,401	-	yagZ	b0293	0.16	+0.1
309,435	-	yagZ	b0293	0.22	+0.1
309,515	-	yagZ	b0293	0.35	+0.6
309,532	-	yagZ	b0293	0.38	-0.6
309,552	+	yagZ	b0293	0.41	+1.7
309,583	-	yagZ	b0293	0.47	+0.4
309,597	+	yagZ	b0293	0.49	-3.6
309,609	+	yagZ	b0293	0.51	+0.5
309,609	+	yagZ	b0293	0.51	+0.8
309,630	+	yagZ	b0293	0.55	-0.3
309,640	+	yagZ	b0293	0.56	+0.3
309,657	+	yagZ	b0293	0.59	-0.2
309,673	-	yagZ	b0293	0.62	+0.0
309,726	+	yagZ	b0293	0.71	+0.2
309,778	+	yagZ	b0293	0.80	+1.1
309,778	+	yagZ	b0293	0.80	+1.6
309,800	+	yagZ	b0293	0.84	-0.0
309,804	+	yagZ	b0293	0.84	-0.4
309,819	+	yagZ	b0293	0.87	-0.7
309,822	-	yagZ	b0293	0.87	+1.0
309,839	-				+0.2
309,839	-				-0.3
309,841	+				-3.0
309,847	+				+1.6
309,870	+				+0.8
309,934	-				+0.6
309,938	-				+2.7
309,961	+				+0.5
309,961	+				-0.5
310,001	+				+2.1
310,001	+				-0.3
310,006	+				+0.5
310,013	+				+0.1
310,013	+				-0.3
310,013	+				+0.5
310,021	-				+0.3
310,029	-	ykgK	b0294	0.10	-2.1
310,033	-	ykgK	b0294	0.11	-1.6
310,035	-	ykgK	b0294	0.11	-1.1
310,035	-	ykgK	b0294	0.11	-0.1
310,035	-	ykgK	b0294	0.11	-0.8
310,035	-	ykgK	b0294	0.11	+0.2
310,044	-	ykgK	b0294	0.13	-1.7
310,044	-	ykgK	b0294	0.13	-1.7
310,102	+	ykgK	b0294	0.22	+2.1
310,219	-	ykgK	b0294	0.42	+2.1
310,219	-	ykgK	b0294	0.42	+2.1
310,459	-	ykgK	b0294	0.83	+0.5
310,646	-				+2.1
310,829	+				+2.1
310,880	-				+2.1
310,891	-				+2.1
310,891	-				+2.1
310,892	-				+0.1
310,892	-				-1.1
310,894	-				+2.1
310,904	-				+0.5
311,087	-				-0.3
311,091	-				+1.7
311,100	+				-0.3
311,100	+				+1.2
311,114	-				+2.1
311,138	-				+1.4
311,172	-				-0.3
311,172	-				-0.9
311,194	-				-2.4
311,222	-				-1.0
311,222	-				+0.7
311,256	+				+0.1
311,260	+				-0.3
311,260	+				+0.2
311,352	+				-0.4
311,360	+	ykgL	b0295	0.11	+0.2
311,360	+	ykgL	b0295	0.11	-1.1
311,364	-	ykgL	b0295	0.12	-2.3
311,370	+	ykgL	b0295	0.15	-0.5
311,380	+	ykgL	b0295	0.19	+0.3
311,385	+	ykgL	b0295	0.21	-0.0
311,385	+	ykgL	b0295	0.21	-0.3
311,396	+	ykgL	b0295	0.26	+0.1
311,396	+	ykgL	b0295	0.26	+0.5
311,402	+	ykgL	b0295	0.29	+0.2
311,402	+	ykgL	b0295	0.29	+0.2
311,418	-	ykgL	b0295	0.36	-1.8
311,470	+	ykgL	b0295	0.59	+0.9
311,470	+	ykgL	b0295	0.59	+0.1
311,525	+	ykgL	b0295	0.83	+2.1