Experiment: Growth with Chloride 500 mM; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0648 and PP_0649 are separated by 87 nucleotides PP_0649 and PP_0650 are separated by 27 nucleotides PP_0650 and PP_0651 are separated by 108 nucleotides PP_0651 and PP_5453 are separated by 833 nucleotides
PP_0648: PP_0648 - conserved protein of unknown function, at 757,379 to 757,834
_0648
PP_0649: PP_0649 - MutT/nudix family protein, at 757,922 to 758,332
_0649
PP_0650: PP_0650 - conserved protein of unknown function, at 758,360 to 758,470
_0650
PP_0651: PP_0651 - Acetyltransferase, GNAT family, at 758,579 to 759,028
_0651
PP_5453: PP_5453 - conserved protein of unknown function, at 759,862 to 760,089
_5453
Position (kb) 758
759
760 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 757.594 kb on + strand, within PP_0648 at 757.595 kb on - strand, within PP_0648 at 757.606 kb on + strand, within PP_0648 at 757.606 kb on + strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.607 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.614 kb on - strand, within PP_0648 at 757.633 kb on + strand, within PP_0648 at 757.634 kb on - strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.637 kb on + strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.638 kb on - strand, within PP_0648 at 757.639 kb on + strand, within PP_0648 at 757.639 kb on + strand, within PP_0648 at 757.640 kb on - strand, within PP_0648 at 757.640 kb on - strand, within PP_0648 at 757.641 kb on - strand, within PP_0648 at 757.673 kb on + strand, within PP_0648 at 757.674 kb on - strand, within PP_0648 at 757.674 kb on - strand, within PP_0648 at 757.693 kb on + strand, within PP_0648 at 757.756 kb on + strand, within PP_0648 at 757.756 kb on + strand, within PP_0648 at 757.757 kb on - strand, within PP_0648 at 757.757 kb on - strand, within PP_0648 at 757.767 kb on + strand, within PP_0648 at 757.767 kb on + strand, within PP_0648 at 757.803 kb on - strand at 757.838 kb on + strand at 757.880 kb on + strand at 757.880 kb on + strand at 757.880 kb on + strand at 757.900 kb on + strand at 757.900 kb on + strand at 757.900 kb on + strand at 757.947 kb on + strand at 757.947 kb on + strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.948 kb on - strand at 757.980 kb on + strand, within PP_0649 at 757.998 kb on + strand, within PP_0649 at 757.998 kb on + strand, within PP_0649 at 757.999 kb on - strand, within PP_0649 at 758.087 kb on - strand, within PP_0649 at 758.087 kb on - strand, within PP_0649 at 758.189 kb on - strand, within PP_0649 at 758.189 kb on - strand, within PP_0649 at 758.306 kb on - strand at 758.330 kb on + strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.331 kb on - strand at 758.340 kb on + strand at 758.340 kb on + strand at 758.341 kb on - strand at 758.341 kb on - strand at 758.358 kb on - strand at 758.384 kb on + strand, within PP_0650 at 758.384 kb on + strand, within PP_0650 at 758.385 kb on - strand, within PP_0650 at 758.385 kb on - strand, within PP_0650 at 758.387 kb on + strand, within PP_0650 at 758.387 kb on + strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.388 kb on - strand, within PP_0650 at 758.446 kb on - strand, within PP_0650 at 758.446 kb on - strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.451 kb on + strand, within PP_0650 at 758.452 kb on - strand, within PP_0650 at 758.452 kb on - strand, within PP_0650 at 758.486 kb on + strand at 758.486 kb on + strand at 758.486 kb on + strand at 758.514 kb on + strand at 758.514 kb on + strand at 758.515 kb on - strand at 758.538 kb on + strand at 758.539 kb on - strand at 758.539 kb on - strand at 758.539 kb on - strand at 758.560 kb on - strand at 758.562 kb on - strand at 758.626 kb on - strand, within PP_0651 at 758.626 kb on - strand, within PP_0651 at 758.638 kb on + strand, within PP_0651 at 758.638 kb on + strand, within PP_0651 at 758.639 kb on - strand, within PP_0651 at 758.640 kb on + strand, within PP_0651 at 758.640 kb on + strand, within PP_0651 at 758.737 kb on + strand, within PP_0651 at 758.738 kb on - strand at 758.757 kb on + strand, within PP_0651 at 758.757 kb on + strand, within PP_0651 at 758.758 kb on - strand, within PP_0651 at 758.758 kb on - strand, within PP_0651 at 758.763 kb on + strand, within PP_0651 at 758.764 kb on - strand, within PP_0651 at 758.881 kb on + strand, within PP_0651 at 758.881 kb on + strand, within PP_0651 at 758.881 kb on + strand, within PP_0651 at 758.883 kb on + strand, within PP_0651 at 758.883 kb on + strand, within PP_0651 at 758.883 kb on + strand, within PP_0651 at 758.884 kb on - strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.913 kb on + strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.914 kb on - strand, within PP_0651 at 758.948 kb on + strand, within PP_0651 at 758.948 kb on + strand, within PP_0651 at 759.011 kb on - strand at 759.026 kb on + strand at 759.026 kb on + strand at 759.027 kb on - strand at 759.042 kb on + strand at 759.042 kb on + strand at 759.047 kb on + strand at 759.048 kb on - strand at 759.048 kb on - strand at 759.070 kb on - strand at 759.070 kb on - strand at 759.092 kb on + strand at 759.092 kb on + strand at 759.092 kb on + strand at 759.092 kb on + strand at 759.093 kb on - strand at 759.093 kb on - strand at 759.093 kb on - strand at 759.093 kb on - strand at 759.096 kb on + strand at 759.096 kb on + strand at 759.096 kb on + strand at 759.097 kb on - strand at 759.097 kb on - strand at 759.118 kb on + strand at 759.118 kb on + strand at 759.119 kb on - strand at 759.119 kb on - strand at 759.202 kb on + strand at 759.226 kb on + strand at 759.276 kb on + strand at 759.277 kb on - strand at 759.288 kb on + strand at 759.288 kb on + strand at 759.288 kb on + strand at 759.289 kb on - strand at 759.294 kb on + strand at 759.294 kb on + strand at 759.295 kb on - strand at 759.310 kb on + strand at 759.310 kb on + strand at 759.310 kb on + strand at 759.310 kb on + strand at 759.311 kb on - strand at 759.313 kb on + strand at 759.313 kb on + strand at 759.374 kb on + strand at 759.375 kb on - strand at 759.375 kb on - strand at 759.396 kb on + strand at 759.419 kb on + strand at 759.434 kb on - strand at 759.607 kb on + strand at 759.608 kb on - strand at 759.608 kb on - strand at 759.675 kb on + strand at 759.675 kb on + strand at 759.675 kb on + strand at 759.675 kb on + strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.676 kb on - strand at 759.760 kb on - strand at 759.810 kb on + strand at 759.811 kb on - strand at 759.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM; with MOPS remove 757,594 + PP_0648 0.47 +2.6 757,595 - PP_0648 0.47 +0.8 757,606 + PP_0648 0.50 +0.3 757,606 + PP_0648 0.50 +0.9 757,607 - PP_0648 0.50 -0.5 757,607 - PP_0648 0.50 -0.8 757,607 - PP_0648 0.50 -0.2 757,607 - PP_0648 0.50 +0.1 757,614 - PP_0648 0.52 +0.5 757,614 - PP_0648 0.52 +1.3 757,614 - PP_0648 0.52 -0.2 757,633 + PP_0648 0.56 +1.1 757,634 - PP_0648 0.56 -1.9 757,637 + PP_0648 0.57 +0.0 757,637 + PP_0648 0.57 -0.0 757,637 + PP_0648 0.57 +0.3 757,637 + PP_0648 0.57 +0.5 757,637 + PP_0648 0.57 -1.6 757,637 + PP_0648 0.57 -0.1 757,637 + PP_0648 0.57 +0.5 757,638 - PP_0648 0.57 +0.3 757,638 - PP_0648 0.57 +0.5 757,638 - PP_0648 0.57 +0.1 757,638 - PP_0648 0.57 +0.4 757,638 - PP_0648 0.57 +0.5 757,638 - PP_0648 0.57 +0.3 757,638 - PP_0648 0.57 +0.7 757,638 - PP_0648 0.57 -0.5 757,638 - PP_0648 0.57 +0.4 757,639 + PP_0648 0.57 -1.5 757,639 + PP_0648 0.57 +0.6 757,640 - PP_0648 0.57 +1.2 757,640 - PP_0648 0.57 -2.7 757,641 - PP_0648 0.57 -0.6 757,673 + PP_0648 0.64 +0.3 757,674 - PP_0648 0.65 +0.9 757,674 - PP_0648 0.65 +1.0 757,693 + PP_0648 0.69 -0.6 757,756 + PP_0648 0.83 +0.9 757,756 + PP_0648 0.83 +1.5 757,757 - PP_0648 0.83 -3.0 757,757 - PP_0648 0.83 -1.8 757,767 + PP_0648 0.85 -0.8 757,767 + PP_0648 0.85 -0.3 757,803 - +0.9 757,838 + -0.8 757,880 + -0.0 757,880 + +1.8 757,880 + +2.0 757,900 + -0.7 757,900 + +0.3 757,900 + -0.5 757,947 + -0.0 757,947 + +1.0 757,948 - +0.2 757,948 - +0.3 757,948 - -1.3 757,948 - +0.7 757,948 - -1.4 757,980 + PP_0649 0.14 -0.9 757,998 + PP_0649 0.18 -0.1 757,998 + PP_0649 0.18 +0.2 757,999 - PP_0649 0.19 -1.9 758,087 - PP_0649 0.40 -0.4 758,087 - PP_0649 0.40 +0.1 758,189 - PP_0649 0.65 +1.4 758,189 - PP_0649 0.65 +0.2 758,306 - -2.5 758,330 + -1.1 758,331 - -0.3 758,331 - +1.3 758,331 - -1.2 758,331 - +1.2 758,340 + -0.0 758,340 + -1.3 758,341 - +1.0 758,341 - +0.4 758,358 - +0.2 758,384 + PP_0650 0.22 -3.3 758,384 + PP_0650 0.22 +0.9 758,385 - PP_0650 0.23 +0.0 758,385 - PP_0650 0.23 +0.4 758,387 + PP_0650 0.24 -2.2 758,387 + PP_0650 0.24 -0.2 758,388 - PP_0650 0.25 -0.7 758,388 - PP_0650 0.25 -1.2 758,388 - PP_0650 0.25 +1.3 758,388 - PP_0650 0.25 -0.5 758,446 - PP_0650 0.77 -0.6 758,446 - PP_0650 0.77 +1.5 758,451 + PP_0650 0.82 -0.5 758,451 + PP_0650 0.82 -3.9 758,451 + PP_0650 0.82 +0.6 758,452 - PP_0650 0.83 +0.4 758,452 - PP_0650 0.83 -0.5 758,486 + +0.9 758,486 + -1.3 758,486 + +0.5 758,514 + -1.3 758,514 + -0.2 758,515 - +1.0 758,538 + -0.3 758,539 - -0.1 758,539 - +0.3 758,539 - +0.9 758,560 - -2.1 758,562 - -0.4 758,626 - PP_0651 0.10 -1.0 758,626 - PP_0651 0.10 -3.3 758,638 + PP_0651 0.13 +1.4 758,638 + PP_0651 0.13 -1.8 758,639 - PP_0651 0.13 +0.1 758,640 + PP_0651 0.14 -2.2 758,640 + PP_0651 0.14 -1.5 758,737 + PP_0651 0.35 -0.3 758,738 - +3.0 758,757 + PP_0651 0.40 +0.7 758,757 + PP_0651 0.40 -0.4 758,758 - PP_0651 0.40 -2.0 758,758 - PP_0651 0.40 -0.6 758,763 + PP_0651 0.41 -0.1 758,764 - PP_0651 0.41 -0.6 758,881 + PP_0651 0.67 -1.1 758,881 + PP_0651 0.67 -4.0 758,881 + PP_0651 0.67 -1.3 758,883 + PP_0651 0.68 +0.3 758,883 + PP_0651 0.68 +1.7 758,883 + PP_0651 0.68 +0.3 758,884 - PP_0651 0.68 -1.0 758,913 + PP_0651 0.74 +0.7 758,913 + PP_0651 0.74 -1.3 758,913 + PP_0651 0.74 +0.3 758,913 + PP_0651 0.74 -1.0 758,913 + PP_0651 0.74 +0.6 758,914 - PP_0651 0.74 -2.7 758,914 - PP_0651 0.74 +0.1 758,914 - PP_0651 0.74 +2.2 758,914 - PP_0651 0.74 -1.3 758,914 - PP_0651 0.74 +0.2 758,948 + PP_0651 0.82 -0.7 758,948 + PP_0651 0.82 +1.2 759,011 - -1.6 759,026 + +0.6 759,026 + +0.1 759,027 - -3.7 759,042 + +4.2 759,042 + +0.7 759,047 + -1.5 759,048 - +0.1 759,048 - +1.9 759,070 - -0.8 759,070 - -0.8 759,092 + +1.1 759,092 + +1.3 759,092 + +1.1 759,092 + -0.0 759,093 - -0.2 759,093 - -1.8 759,093 - -0.5 759,093 - -1.2 759,096 + -0.9 759,096 + +1.1 759,096 + +0.4 759,097 - +0.4 759,097 - -1.1 759,118 + -1.7 759,118 + +0.2 759,119 - -0.3 759,119 - -0.7 759,202 + +0.9 759,226 + +0.7 759,276 + +0.7 759,277 - +0.1 759,288 + +0.7 759,288 + +1.1 759,288 + +1.1 759,289 - +1.2 759,294 + -2.9 759,294 + +0.8 759,295 - -1.3 759,310 + +0.8 759,310 + +0.1 759,310 + +1.1 759,310 + -1.2 759,311 - -0.3 759,313 + +1.3 759,313 + +0.9 759,374 + +0.9 759,375 - -2.2 759,375 - +1.4 759,396 + -1.2 759,419 + +0.9 759,434 - +0.7 759,607 + +1.4 759,608 - +0.9 759,608 - +0.0 759,675 + +0.6 759,675 + -0.7 759,675 + -0.3 759,675 + +0.2 759,676 - -1.8 759,676 - -2.5 759,676 - +0.4 759,676 - +0.0 759,676 - -0.2 759,676 - +0.2 759,760 - +1.0 759,810 + +0.9 759,811 - +0.2 759,811 - +1.2
Or see this region's nucleotide sequence