Experiment: N4_phage 0.009375 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glgS and yqiJ are separated by 268 nucleotides yqiJ and yqiK are separated by 26 nucleotides yqiK and rygD are separated by 197 nucleotides rygD and ibsD overlap by 100 nucleotides ibsD and rygE are separated by 273 nucleotides rygE and ibsE overlap by 100 nucleotides ibsE and rfaE are separated by 119 nucleotides
b3049: glgS - glycogen synthesis protein GlgS (NCBI), at 3,189,761 to 3,189,961
glgS
b3050: yqiJ - predicted inner membrane protein (NCBI), at 3,190,230 to 3,190,859
yqiJ
b3051: yqiK - hypothetical protein (NCBI), at 3,190,886 to 3,192,547
yqiK
b4447: rygD - Putative sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,192,745 to 3,192,887
rygD
b4664: ibsD - toxic membrane protein (RefSeq), at 3,192,788 to 3,192,847
ibsD
b4611: rygE - Putative sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,193,121 to 3,193,262
rygE
b4666: ibsE - toxic membrane protein (RefSeq), at 3,193,163 to 3,193,222
ibsE
b3052: rfaE - fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI), at 3,193,342 to 3,194,775
rfaE
Position (kb)
3190
3191
3192
3193 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3189.891 kb on + strand, within glgS at 3189.895 kb on - strand, within glgS at 3189.899 kb on - strand, within glgS at 3189.965 kb on - strand at 3189.967 kb on - strand at 3189.967 kb on - strand at 3189.979 kb on + strand at 3189.987 kb on - strand at 3189.990 kb on + strand at 3189.990 kb on + strand at 3189.995 kb on - strand at 3189.995 kb on - strand at 3190.023 kb on + strand at 3190.031 kb on - strand at 3190.031 kb on - strand at 3190.031 kb on - strand at 3190.036 kb on - strand at 3190.041 kb on - strand at 3190.041 kb on - strand at 3190.041 kb on - strand at 3190.047 kb on + strand at 3190.133 kb on - strand at 3190.138 kb on - strand at 3190.143 kb on - strand at 3190.143 kb on - strand at 3190.143 kb on - strand at 3190.150 kb on + strand at 3190.150 kb on + strand at 3190.207 kb on + strand at 3190.207 kb on + strand at 3190.215 kb on + strand at 3190.255 kb on - strand at 3190.276 kb on + strand at 3190.280 kb on - strand at 3190.284 kb on - strand at 3190.287 kb on - strand at 3190.289 kb on - strand at 3190.316 kb on + strand, within yqiJ at 3190.318 kb on + strand, within yqiJ at 3190.318 kb on + strand, within yqiJ at 3190.324 kb on + strand, within yqiJ at 3190.324 kb on - strand, within yqiJ at 3190.324 kb on - strand, within yqiJ at 3190.326 kb on + strand, within yqiJ at 3190.326 kb on + strand, within yqiJ at 3190.326 kb on - strand, within yqiJ at 3190.326 kb on - strand, within yqiJ at 3190.328 kb on + strand, within yqiJ at 3190.328 kb on + strand, within yqiJ at 3190.328 kb on + strand, within yqiJ at 3190.328 kb on + strand, within yqiJ at 3190.329 kb on + strand, within yqiJ at 3190.329 kb on - strand, within yqiJ at 3190.331 kb on + strand, within yqiJ at 3190.332 kb on + strand, within yqiJ at 3190.332 kb on - strand, within yqiJ at 3190.333 kb on + strand, within yqiJ at 3190.333 kb on + strand, within yqiJ at 3190.334 kb on + strand, within yqiJ at 3190.334 kb on - strand, within yqiJ at 3190.336 kb on - strand, within yqiJ at 3190.336 kb on - strand, within yqiJ at 3190.342 kb on - strand, within yqiJ at 3190.344 kb on + strand, within yqiJ at 3190.344 kb on + strand, within yqiJ at 3190.349 kb on + strand, within yqiJ at 3190.349 kb on + strand, within yqiJ at 3190.353 kb on - strand, within yqiJ at 3190.353 kb on - strand, within yqiJ at 3190.370 kb on + strand, within yqiJ at 3190.370 kb on + strand, within yqiJ at 3190.370 kb on + strand, within yqiJ at 3190.372 kb on - strand, within yqiJ at 3190.372 kb on - strand, within yqiJ at 3190.378 kb on - strand, within yqiJ at 3190.381 kb on - strand, within yqiJ at 3190.381 kb on - strand, within yqiJ at 3190.387 kb on + strand, within yqiJ at 3190.387 kb on + strand, within yqiJ at 3190.400 kb on - strand, within yqiJ at 3190.425 kb on + strand, within yqiJ at 3190.440 kb on + strand, within yqiJ at 3190.442 kb on + strand, within yqiJ at 3190.442 kb on - strand, within yqiJ at 3190.448 kb on - strand, within yqiJ at 3190.448 kb on - strand, within yqiJ at 3190.480 kb on + strand, within yqiJ at 3190.482 kb on + strand, within yqiJ at 3190.482 kb on + strand, within yqiJ at 3190.482 kb on + strand, within yqiJ at 3190.482 kb on + strand, within yqiJ at 3190.482 kb on + strand, within yqiJ at 3190.485 kb on - strand, within yqiJ at 3190.486 kb on - strand, within yqiJ at 3190.486 kb on - strand, within yqiJ at 3190.490 kb on + strand, within yqiJ at 3190.490 kb on + strand, within yqiJ at 3190.492 kb on - strand, within yqiJ at 3190.493 kb on + strand, within yqiJ at 3190.493 kb on + strand, within yqiJ at 3190.496 kb on + strand, within yqiJ at 3190.498 kb on + strand, within yqiJ at 3190.498 kb on + strand, within yqiJ at 3190.498 kb on + strand, within yqiJ at 3190.499 kb on + strand, within yqiJ at 3190.500 kb on + strand, within yqiJ at 3190.500 kb on + strand, within yqiJ at 3190.502 kb on - strand, within yqiJ at 3190.507 kb on - strand, within yqiJ at 3190.513 kb on + strand, within yqiJ at 3190.513 kb on + strand, within yqiJ at 3190.513 kb on + strand, within yqiJ at 3190.513 kb on + strand, within yqiJ at 3190.518 kb on - strand, within yqiJ at 3190.521 kb on + strand, within yqiJ at 3190.521 kb on + strand, within yqiJ at 3190.521 kb on + strand, within yqiJ at 3190.521 kb on + strand, within yqiJ at 3190.521 kb on + strand, within yqiJ at 3190.526 kb on - strand, within yqiJ at 3190.532 kb on + strand, within yqiJ at 3190.549 kb on + strand, within yqiJ at 3190.549 kb on + strand, within yqiJ at 3190.574 kb on + strand, within yqiJ at 3190.611 kb on + strand, within yqiJ at 3190.611 kb on + strand, within yqiJ at 3190.615 kb on + strand, within yqiJ at 3190.716 kb on + strand, within yqiJ at 3190.716 kb on + strand, within yqiJ at 3190.970 kb on - strand at 3190.986 kb on - strand at 3191.000 kb on + strand at 3191.020 kb on - strand at 3191.029 kb on - strand at 3191.029 kb on - strand at 3191.055 kb on - strand, within yqiK at 3191.063 kb on - strand, within yqiK at 3191.063 kb on - strand, within yqiK at 3191.095 kb on + strand, within yqiK at 3191.103 kb on - strand, within yqiK at 3191.132 kb on + strand, within yqiK at 3191.132 kb on + strand, within yqiK at 3191.133 kb on - strand, within yqiK at 3191.144 kb on - strand, within yqiK at 3191.156 kb on + strand, within yqiK at 3191.177 kb on - strand, within yqiK at 3191.180 kb on - strand, within yqiK at 3191.239 kb on - strand, within yqiK at 3191.239 kb on - strand, within yqiK at 3191.324 kb on - strand, within yqiK at 3191.678 kb on + strand, within yqiK at 3191.686 kb on - strand, within yqiK at 3191.722 kb on - strand, within yqiK at 3191.727 kb on + strand, within yqiK at 3191.819 kb on + strand, within yqiK at 3191.819 kb on + strand, within yqiK at 3191.842 kb on + strand, within yqiK at 3191.930 kb on - strand, within yqiK at 3191.989 kb on + strand, within yqiK at 3192.012 kb on + strand, within yqiK at 3192.171 kb on - strand, within yqiK at 3192.171 kb on - strand, within yqiK at 3192.180 kb on + strand, within yqiK at 3192.187 kb on + strand, within yqiK at 3192.193 kb on - strand, within yqiK at 3192.267 kb on + strand, within yqiK at 3192.267 kb on + strand, within yqiK at 3192.300 kb on - strand, within yqiK at 3192.427 kb on - strand at 3192.563 kb on - strand at 3192.698 kb on - strand at 3192.698 kb on - strand at 3192.740 kb on - strand at 3192.943 kb on + strand at 3193.398 kb on + strand at 3193.441 kb on - strand at 3193.545 kb on + strand, within rfaE
Per-strain Table
Position Strand Gene LocusTag Fraction N4_phage 0.009375 MOI TC1 remove 3,189,891 + glgS b3049 0.65 +0.8 3,189,895 - glgS b3049 0.67 +0.5 3,189,899 - glgS b3049 0.69 -0.1 3,189,965 - +0.5 3,189,967 - +0.6 3,189,967 - -0.1 3,189,979 + -0.0 3,189,987 - +0.3 3,189,990 + +0.3 3,189,990 + +0.7 3,189,995 - -1.2 3,189,995 - -0.2 3,190,023 + +2.6 3,190,031 - +1.0 3,190,031 - -0.7 3,190,031 - +1.1 3,190,036 - +0.0 3,190,041 - -0.1 3,190,041 - -0.5 3,190,041 - -1.2 3,190,047 + +2.2 3,190,133 - +0.7 3,190,138 - +0.3 3,190,143 - -0.5 3,190,143 - +0.6 3,190,143 - -0.4 3,190,150 + -0.1 3,190,150 + +1.2 3,190,207 + -0.9 3,190,207 + -3.5 3,190,215 + +0.1 3,190,255 - +1.2 3,190,276 + -1.8 3,190,280 - -1.8 3,190,284 - +0.8 3,190,287 - +0.6 3,190,289 - -2.1 3,190,316 + yqiJ b3050 0.14 -1.0 3,190,318 + yqiJ b3050 0.14 -0.8 3,190,318 + yqiJ b3050 0.14 +0.2 3,190,324 + yqiJ b3050 0.15 +0.9 3,190,324 - yqiJ b3050 0.15 -1.3 3,190,324 - yqiJ b3050 0.15 -1.5 3,190,326 + yqiJ b3050 0.15 +0.4 3,190,326 + yqiJ b3050 0.15 -0.6 3,190,326 - yqiJ b3050 0.15 -2.1 3,190,326 - yqiJ b3050 0.15 +0.4 3,190,328 + yqiJ b3050 0.16 -0.2 3,190,328 + yqiJ b3050 0.16 +0.1 3,190,328 + yqiJ b3050 0.16 -0.1 3,190,328 + yqiJ b3050 0.16 -2.1 3,190,329 + yqiJ b3050 0.16 +0.3 3,190,329 - yqiJ b3050 0.16 -0.3 3,190,331 + yqiJ b3050 0.16 +0.7 3,190,332 + yqiJ b3050 0.16 +0.8 3,190,332 - yqiJ b3050 0.16 -2.0 3,190,333 + yqiJ b3050 0.16 -0.7 3,190,333 + yqiJ b3050 0.16 -2.0 3,190,334 + yqiJ b3050 0.17 -0.7 3,190,334 - yqiJ b3050 0.17 +2.2 3,190,336 - yqiJ b3050 0.17 +0.8 3,190,336 - yqiJ b3050 0.17 +0.1 3,190,342 - yqiJ b3050 0.18 +0.7 3,190,344 + yqiJ b3050 0.18 +0.7 3,190,344 + yqiJ b3050 0.18 +1.9 3,190,349 + yqiJ b3050 0.19 +0.8 3,190,349 + yqiJ b3050 0.19 -0.9 3,190,353 - yqiJ b3050 0.20 -1.0 3,190,353 - yqiJ b3050 0.20 +3.2 3,190,370 + yqiJ b3050 0.22 +0.1 3,190,370 + yqiJ b3050 0.22 +0.6 3,190,370 + yqiJ b3050 0.22 +0.3 3,190,372 - yqiJ b3050 0.23 -1.6 3,190,372 - yqiJ b3050 0.23 -1.4 3,190,378 - yqiJ b3050 0.23 +0.6 3,190,381 - yqiJ b3050 0.24 +0.6 3,190,381 - yqiJ b3050 0.24 +1.4 3,190,387 + yqiJ b3050 0.25 -0.6 3,190,387 + yqiJ b3050 0.25 -0.1 3,190,400 - yqiJ b3050 0.27 -1.1 3,190,425 + yqiJ b3050 0.31 -1.0 3,190,440 + yqiJ b3050 0.33 +0.9 3,190,442 + yqiJ b3050 0.34 -0.8 3,190,442 - yqiJ b3050 0.34 +1.0 3,190,448 - yqiJ b3050 0.35 -0.5 3,190,448 - yqiJ b3050 0.35 -0.8 3,190,480 + yqiJ b3050 0.40 -0.1 3,190,482 + yqiJ b3050 0.40 -2.2 3,190,482 + yqiJ b3050 0.40 -0.6 3,190,482 + yqiJ b3050 0.40 -1.2 3,190,482 + yqiJ b3050 0.40 -0.3 3,190,482 + yqiJ b3050 0.40 -1.0 3,190,485 - yqiJ b3050 0.40 -1.1 3,190,486 - yqiJ b3050 0.41 -0.8 3,190,486 - yqiJ b3050 0.41 +0.2 3,190,490 + yqiJ b3050 0.41 +0.6 3,190,490 + yqiJ b3050 0.41 +0.2 3,190,492 - yqiJ b3050 0.42 +2.2 3,190,493 + yqiJ b3050 0.42 +0.3 3,190,493 + yqiJ b3050 0.42 -0.9 3,190,496 + yqiJ b3050 0.42 +2.2 3,190,498 + yqiJ b3050 0.43 +1.5 3,190,498 + yqiJ b3050 0.43 +0.2 3,190,498 + yqiJ b3050 0.43 +0.6 3,190,499 + yqiJ b3050 0.43 +0.4 3,190,500 + yqiJ b3050 0.43 -0.4 3,190,500 + yqiJ b3050 0.43 +0.6 3,190,502 - yqiJ b3050 0.43 +0.9 3,190,507 - yqiJ b3050 0.44 +0.2 3,190,513 + yqiJ b3050 0.45 +1.9 3,190,513 + yqiJ b3050 0.45 +0.1 3,190,513 + yqiJ b3050 0.45 -1.2 3,190,513 + yqiJ b3050 0.45 -1.1 3,190,518 - yqiJ b3050 0.46 +2.2 3,190,521 + yqiJ b3050 0.46 -0.4 3,190,521 + yqiJ b3050 0.46 +1.2 3,190,521 + yqiJ b3050 0.46 -0.3 3,190,521 + yqiJ b3050 0.46 -0.4 3,190,521 + yqiJ b3050 0.46 +0.1 3,190,526 - yqiJ b3050 0.47 +2.2 3,190,532 + yqiJ b3050 0.48 +1.0 3,190,549 + yqiJ b3050 0.51 -0.1 3,190,549 + yqiJ b3050 0.51 -2.7 3,190,574 + yqiJ b3050 0.55 +0.1 3,190,611 + yqiJ b3050 0.60 +0.6 3,190,611 + yqiJ b3050 0.60 -1.0 3,190,615 + yqiJ b3050 0.61 +2.2 3,190,716 + yqiJ b3050 0.77 +0.2 3,190,716 + yqiJ b3050 0.77 +2.2 3,190,970 - +0.1 3,190,986 - -0.1 3,191,000 + +2.2 3,191,020 - +2.3 3,191,029 - -1.0 3,191,029 - +0.0 3,191,055 - yqiK b3051 0.10 -0.3 3,191,063 - yqiK b3051 0.11 +0.0 3,191,063 - yqiK b3051 0.11 +1.8 3,191,095 + yqiK b3051 0.13 -1.8 3,191,103 - yqiK b3051 0.13 -0.2 3,191,132 + yqiK b3051 0.15 +2.6 3,191,132 + yqiK b3051 0.15 +0.1 3,191,133 - yqiK b3051 0.15 +1.1 3,191,144 - yqiK b3051 0.16 +0.0 3,191,156 + yqiK b3051 0.16 +0.7 3,191,177 - yqiK b3051 0.18 +0.3 3,191,180 - yqiK b3051 0.18 -0.1 3,191,239 - yqiK b3051 0.21 -0.3 3,191,239 - yqiK b3051 0.21 -1.1 3,191,324 - yqiK b3051 0.26 -2.4 3,191,678 + yqiK b3051 0.48 -0.0 3,191,686 - yqiK b3051 0.48 +0.6 3,191,722 - yqiK b3051 0.50 +0.7 3,191,727 + yqiK b3051 0.51 -0.5 3,191,819 + yqiK b3051 0.56 -0.4 3,191,819 + yqiK b3051 0.56 -0.0 3,191,842 + yqiK b3051 0.58 +0.3 3,191,930 - yqiK b3051 0.63 +0.7 3,191,989 + yqiK b3051 0.66 +0.9 3,192,012 + yqiK b3051 0.68 +1.0 3,192,171 - yqiK b3051 0.77 +0.6 3,192,171 - yqiK b3051 0.77 +1.4 3,192,180 + yqiK b3051 0.78 +0.2 3,192,187 + yqiK b3051 0.78 +0.9 3,192,193 - yqiK b3051 0.79 +0.1 3,192,267 + yqiK b3051 0.83 +0.7 3,192,267 + yqiK b3051 0.83 -2.3 3,192,300 - yqiK b3051 0.85 +0.2 3,192,427 - -0.1 3,192,563 - -0.6 3,192,698 - +1.0 3,192,698 - +0.1 3,192,740 - +0.1 3,192,943 + +0.9 3,193,398 + -0.9 3,193,441 - +1.5 3,193,545 + rfaE b3052 0.14 -0.8
Or see this region's nucleotide sequence