Strain Fitness in Escherichia coli BW25113 around b1655

Experiment: N4_phage 0.009375 MOI TC1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlhr and ydhD are separated by 50 nucleotidesydhD and ydhO are separated by 333 nucleotidesydhO and sodB are separated by 127 nucleotidessodB and ydhP are separated by 161 nucleotides b1653: lhr - predicted ATP-dependent helicase (NCBI), at 1,727,111 to 1,731,727 lhr b1654: ydhD - hypothetical protein (NCBI), at 1,731,778 to 1,732,125 ydhD b1655: ydhO - predicted lipoprotein (NCBI), at 1,732,459 to 1,733,274 ydhO b1656: sodB - superoxide dismutase, Fe (NCBI), at 1,733,402 to 1,733,983 sodB b1657: ydhP - predicted transporter (NCBI), at 1,734,145 to 1,735,314 ydhP Position (kb) 1732 1733 1734Strain fitness (log2 ratio) -3 -2 -1 0 1at 1731.640 kb on - strandat 1731.728 kb on - strandat 1732.385 kb on - strandat 1733.172 kb on + strand, within ydhOat 1733.172 kb on + strand, within ydhOat 1733.225 kb on + strandat 1733.425 kb on + strandat 1733.522 kb on + strand, within sodBat 1733.612 kb on + strand, within sodBat 1733.702 kb on + strand, within sodBat 1733.803 kb on - strand, within sodBat 1733.803 kb on - strand, within sodBat 1734.037 kb on - strandat 1734.128 kb on - strandat 1734.128 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N4_phage 0.009375 MOI TC1
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1,731,640 - +0.0
1,731,728 - -1.4
1,732,385 - -0.1
1,733,172 + ydhO b1655 0.87 +0.6
1,733,172 + ydhO b1655 0.87 +0.9
1,733,225 + -3.5
1,733,425 + +1.0
1,733,522 + sodB b1656 0.21 -0.4
1,733,612 + sodB b1656 0.36 +0.9
1,733,702 + sodB b1656 0.52 -0.3
1,733,803 - sodB b1656 0.69 +0.7
1,733,803 - sodB b1656 0.69 -0.7
1,734,037 - -1.3
1,734,128 - -2.6
1,734,128 - -1.5

Or see this region's nucleotide sequence