Experiment: Growth with Chloride 500 mM; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0559 and PP_0560 are separated by 99 nucleotides PP_0560 and PP_0561 are separated by 179 nucleotides PP_0561 and PP_0562 are separated by 117 nucleotides
PP_0559: PP_0559 - acetyl-CoA carboxylase - biotin carboxyl carrier subunit, at 649,695 to 650,156
_0559
PP_0560: PP_0560 - 3-dehydroquinate dehydratase 1, type II, at 650,256 to 650,711
_0560
PP_0561: PP_0561 - thiol/disulfide interchange protein 1, at 650,891 to 652,663
_0561
PP_0562: PP_0562 - Methyl-accepting chemotaxis protein CtpL, at 652,781 to 654,742
_0562
Position (kb) 650
651
652
653 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 650.767 kb on - strand at 650.831 kb on - strand at 650.831 kb on - strand at 650.846 kb on + strand at 650.893 kb on - strand at 650.894 kb on + strand at 650.894 kb on + strand at 650.948 kb on + strand at 651.174 kb on + strand, within PP_0561 at 651.263 kb on - strand, within PP_0561 at 651.354 kb on + strand, within PP_0561 at 651.509 kb on + strand, within PP_0561 at 651.548 kb on + strand, within PP_0561 at 651.549 kb on - strand, within PP_0561 at 651.703 kb on + strand, within PP_0561 at 651.704 kb on - strand, within PP_0561 at 651.704 kb on - strand, within PP_0561 at 651.705 kb on + strand, within PP_0561 at 651.705 kb on + strand, within PP_0561 at 651.705 kb on + strand, within PP_0561 at 651.705 kb on + strand, within PP_0561 at 651.706 kb on - strand, within PP_0561 at 651.706 kb on - strand, within PP_0561 at 651.706 kb on - strand, within PP_0561 at 651.706 kb on - strand, within PP_0561 at 651.706 kb on - strand, within PP_0561 at 651.706 kb on - strand, within PP_0561 at 651.706 kb on - strand, within PP_0561 at 651.734 kb on + strand, within PP_0561 at 651.911 kb on + strand, within PP_0561 at 651.932 kb on + strand, within PP_0561 at 652.017 kb on + strand, within PP_0561 at 652.017 kb on + strand, within PP_0561 at 652.214 kb on - strand, within PP_0561 at 652.214 kb on - strand at 652.214 kb on - strand, within PP_0561 at 652.303 kb on + strand, within PP_0561 at 652.303 kb on + strand, within PP_0561 at 652.304 kb on - strand, within PP_0561 at 652.335 kb on + strand, within PP_0561 at 652.335 kb on + strand, within PP_0561 at 652.336 kb on - strand, within PP_0561 at 652.336 kb on - strand, within PP_0561 at 652.336 kb on - strand, within PP_0561 at 652.408 kb on + strand, within PP_0561 at 652.452 kb on + strand, within PP_0561 at 652.452 kb on + strand, within PP_0561 at 652.452 kb on + strand, within PP_0561 at 652.452 kb on + strand, within PP_0561 at 652.452 kb on + strand, within PP_0561 at 652.455 kb on + strand at 652.455 kb on + strand, within PP_0561 at 652.455 kb on + strand, within PP_0561 at 652.455 kb on + strand, within PP_0561 at 652.533 kb on - strand at 652.634 kb on + strand at 652.716 kb on - strand at 652.716 kb on - strand at 652.735 kb on + strand at 652.736 kb on - strand at 652.736 kb on - strand at 652.736 kb on - strand at 652.747 kb on + strand at 652.850 kb on - strand at 653.286 kb on - strand, within PP_0562 at 653.602 kb on + strand, within PP_0562 at 653.603 kb on - strand, within PP_0562 at 653.603 kb on - strand, within PP_0562 at 653.603 kb on - strand, within PP_0562 at 653.634 kb on + strand, within PP_0562
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM; with MOPS remove 650,767 - +1.8 650,831 - +1.0 650,831 - -0.2 650,846 + +0.2 650,893 - +0.7 650,894 + +0.4 650,894 + +1.0 650,948 + -0.9 651,174 + PP_0561 0.16 +0.9 651,263 - PP_0561 0.21 -1.2 651,354 + PP_0561 0.26 -0.2 651,509 + PP_0561 0.35 -0.6 651,548 + PP_0561 0.37 -2.4 651,549 - PP_0561 0.37 -1.2 651,703 + PP_0561 0.46 -0.7 651,704 - PP_0561 0.46 -0.2 651,704 - PP_0561 0.46 -1.3 651,705 + PP_0561 0.46 -1.0 651,705 + PP_0561 0.46 -0.9 651,705 + PP_0561 0.46 -0.0 651,705 + PP_0561 0.46 +0.6 651,706 - PP_0561 0.46 -0.9 651,706 - PP_0561 0.46 +0.4 651,706 - PP_0561 0.46 +0.7 651,706 - PP_0561 0.46 +0.0 651,706 - PP_0561 0.46 -1.4 651,706 - PP_0561 0.46 +0.7 651,706 - PP_0561 0.46 -2.1 651,734 + PP_0561 0.48 -3.4 651,911 + PP_0561 0.58 -0.2 651,932 + PP_0561 0.59 +0.2 652,017 + PP_0561 0.64 +1.7 652,017 + PP_0561 0.64 -2.9 652,214 - PP_0561 0.75 +0.1 652,214 - -0.5 652,214 - PP_0561 0.75 +0.2 652,303 + PP_0561 0.80 +1.2 652,303 + PP_0561 0.80 -1.6 652,304 - PP_0561 0.80 +1.2 652,335 + PP_0561 0.81 -1.2 652,335 + PP_0561 0.81 -0.1 652,336 - PP_0561 0.82 -1.7 652,336 - PP_0561 0.82 -0.3 652,336 - PP_0561 0.82 +0.4 652,408 + PP_0561 0.86 -0.2 652,452 + PP_0561 0.88 -2.5 652,452 + PP_0561 0.88 -1.4 652,452 + PP_0561 0.88 -0.1 652,452 + PP_0561 0.88 -3.0 652,452 + PP_0561 0.88 -1.2 652,455 + -0.9 652,455 + PP_0561 0.88 -0.9 652,455 + PP_0561 0.88 -1.5 652,455 + PP_0561 0.88 -4.2 652,533 - +1.6 652,634 + -1.5 652,716 - -0.5 652,716 - -1.0 652,735 + +1.1 652,736 - -1.8 652,736 - -0.3 652,736 - -1.1 652,747 + +1.7 652,850 - +0.1 653,286 - PP_0562 0.26 -0.4 653,602 + PP_0562 0.42 +0.8 653,603 - PP_0562 0.42 +0.1 653,603 - PP_0562 0.42 +0.4 653,603 - PP_0562 0.42 +0.8 653,634 + PP_0562 0.43 +0.5
Or see this region's nucleotide sequence