Experiment: Growth with Chloride 500 mM; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2130 and PP_2131 are separated by 172 nucleotides PP_2131 and PP_2132 are separated by 107 nucleotides PP_2132 and PP_2133 are separated by 83 nucleotides
PP_2130: PP_2130 - putative Soluble lytic transglycosylase, at 2,429,983 to 2,431,956
_2130
PP_2131: PP_2131 - DNA-binding ATPase involved in replication, at 2,432,129 to 2,434,057
_2131
PP_2132: PP_2132 - Universal stress protein, at 2,434,165 to 2,434,605
_2132
PP_2133: PP_2133 - conserved protein of unknown function, at 2,434,689 to 2,435,153
_2133
Position (kb)
2432
2433
2434
2435 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2431.331 kb on + strand, within PP_2130 at 2431.332 kb on - strand, within PP_2130 at 2431.332 kb on - strand, within PP_2130 at 2431.475 kb on + strand, within PP_2130 at 2431.476 kb on - strand, within PP_2130 at 2431.508 kb on + strand, within PP_2130 at 2431.556 kb on + strand, within PP_2130 at 2431.556 kb on + strand at 2431.556 kb on + strand, within PP_2130 at 2431.557 kb on - strand, within PP_2130 at 2431.560 kb on - strand, within PP_2130 at 2431.560 kb on - strand, within PP_2130 at 2431.573 kb on - strand, within PP_2130 at 2431.721 kb on + strand, within PP_2130 at 2431.721 kb on + strand, within PP_2130 at 2431.758 kb on - strand, within PP_2130 at 2431.767 kb on - strand at 2431.811 kb on + strand at 2431.811 kb on + strand at 2431.811 kb on + strand at 2431.812 kb on - strand at 2431.814 kb on + strand at 2431.814 kb on + strand at 2431.815 kb on - strand at 2431.815 kb on - strand at 2431.815 kb on - strand at 2431.887 kb on + strand at 2431.887 kb on + strand at 2431.887 kb on + strand at 2431.888 kb on - strand at 2431.888 kb on - strand at 2431.891 kb on + strand at 2431.891 kb on + strand at 2431.892 kb on - strand at 2431.892 kb on - strand at 2431.892 kb on - strand at 2431.892 kb on - strand at 2431.892 kb on - strand at 2431.911 kb on + strand at 2431.911 kb on + strand at 2431.911 kb on + strand at 2431.911 kb on + strand at 2431.912 kb on - strand at 2431.912 kb on - strand at 2431.958 kb on - strand at 2431.968 kb on - strand at 2431.975 kb on + strand at 2431.976 kb on - strand at 2431.980 kb on + strand at 2432.018 kb on + strand at 2432.019 kb on - strand at 2432.029 kb on + strand at 2432.029 kb on + strand at 2432.029 kb on + strand at 2432.029 kb on + strand at 2432.030 kb on - strand at 2432.030 kb on - strand at 2432.098 kb on + strand at 2432.099 kb on - strand at 2432.736 kb on + strand, within PP_2131 at 2432.736 kb on + strand, within PP_2131 at 2433.279 kb on - strand, within PP_2131 at 2433.321 kb on + strand, within PP_2131 at 2433.579 kb on - strand, within PP_2131 at 2433.677 kb on + strand, within PP_2131 at 2433.678 kb on - strand, within PP_2131 at 2433.678 kb on - strand, within PP_2131 at 2433.755 kb on - strand, within PP_2131 at 2433.854 kb on + strand, within PP_2131 at 2433.855 kb on - strand, within PP_2131 at 2433.913 kb on + strand at 2433.914 kb on - strand at 2433.953 kb on + strand at 2433.953 kb on + strand at 2433.953 kb on + strand at 2434.023 kb on + strand at 2434.023 kb on + strand at 2434.023 kb on + strand at 2434.024 kb on - strand at 2434.024 kb on - strand at 2434.024 kb on - strand at 2434.453 kb on + strand, within PP_2132 at 2434.453 kb on + strand, within PP_2132 at 2434.453 kb on + strand, within PP_2132 at 2434.454 kb on - strand, within PP_2132 at 2434.504 kb on + strand, within PP_2132 at 2434.598 kb on + strand at 2434.631 kb on - strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.639 kb on + strand at 2434.640 kb on - strand at 2434.709 kb on + strand at 2434.709 kb on + strand at 2434.715 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth with Chloride 500 mM; with MOPS remove 2,431,331 + PP_2130 0.68 +0.1 2,431,332 - PP_2130 0.68 -0.9 2,431,332 - PP_2130 0.68 +0.9 2,431,475 + PP_2130 0.76 -0.8 2,431,476 - PP_2130 0.76 -3.1 2,431,508 + PP_2130 0.77 -1.7 2,431,556 + PP_2130 0.80 -1.0 2,431,556 + -0.7 2,431,556 + PP_2130 0.80 +1.0 2,431,557 - PP_2130 0.80 +0.9 2,431,560 - PP_2130 0.80 -0.2 2,431,560 - PP_2130 0.80 -2.5 2,431,573 - PP_2130 0.81 -0.1 2,431,721 + PP_2130 0.88 +0.4 2,431,721 + PP_2130 0.88 -1.6 2,431,758 - PP_2130 0.90 +0.2 2,431,767 - +0.2 2,431,811 + -0.8 2,431,811 + -2.5 2,431,811 + -3.4 2,431,812 - +1.3 2,431,814 + -0.3 2,431,814 + +0.7 2,431,815 - -0.7 2,431,815 - +1.4 2,431,815 - +0.0 2,431,887 + +0.2 2,431,887 + -0.7 2,431,887 + +1.6 2,431,888 - -0.8 2,431,888 - +0.5 2,431,891 + -0.7 2,431,891 + +0.0 2,431,892 - -1.6 2,431,892 - +0.6 2,431,892 - -0.3 2,431,892 - +0.5 2,431,892 - +0.1 2,431,911 + +0.3 2,431,911 + +1.3 2,431,911 + -0.1 2,431,911 + -1.0 2,431,912 - +1.4 2,431,912 - +0.6 2,431,958 - -0.8 2,431,968 - -0.5 2,431,975 + +0.5 2,431,976 - +0.7 2,431,980 + +1.0 2,432,018 + -0.7 2,432,019 - -1.1 2,432,029 + +1.6 2,432,029 + +0.7 2,432,029 + -0.0 2,432,029 + +0.5 2,432,030 - -2.1 2,432,030 - -0.3 2,432,098 + -1.9 2,432,099 - +0.9 2,432,736 + PP_2131 0.31 +0.4 2,432,736 + PP_2131 0.31 +1.4 2,433,279 - PP_2131 0.60 -0.1 2,433,321 + PP_2131 0.62 -2.8 2,433,579 - PP_2131 0.75 +1.7 2,433,677 + PP_2131 0.80 +0.6 2,433,678 - PP_2131 0.80 +0.9 2,433,678 - PP_2131 0.80 +1.9 2,433,755 - PP_2131 0.84 +0.6 2,433,854 + PP_2131 0.89 +1.1 2,433,855 - PP_2131 0.89 -0.6 2,433,913 + +0.2 2,433,914 - +0.7 2,433,953 + +0.9 2,433,953 + +0.0 2,433,953 + -0.3 2,434,023 + -0.0 2,434,023 + -0.7 2,434,023 + -0.7 2,434,024 - +1.3 2,434,024 - -0.4 2,434,024 - -1.8 2,434,453 + PP_2132 0.65 -0.0 2,434,453 + PP_2132 0.65 -3.9 2,434,453 + PP_2132 0.65 -1.0 2,434,454 - PP_2132 0.66 +1.7 2,434,504 + PP_2132 0.77 -1.4 2,434,598 + -2.6 2,434,631 - +0.1 2,434,639 + +0.3 2,434,639 + -1.1 2,434,639 + -1.0 2,434,639 + -1.1 2,434,639 + -3.7 2,434,640 - +1.8 2,434,709 + -0.2 2,434,709 + -1.9 2,434,715 + +0.5
Or see this region's nucleotide sequence