Experiment: ferulate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0545 and PP_0546 are separated by 254 nucleotides PP_0546 and PP_0547 are separated by 66 nucleotides
PP_0545: PP_0545 - aldehyde dehydrogenase, at 631,920 to 633,440
_0545
PP_0546: PP_0546 - sigma-54 dependent transcriptional regulator, at 633,695 to 635,710
_0546
PP_0547: PP_0547 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 635,777 to 637,126
_0547
Position (kb) 633
634
635
636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 632.717 kb on + strand, within PP_0545 at 632.785 kb on + strand, within PP_0545 at 632.796 kb on + strand, within PP_0545 at 632.797 kb on - strand, within PP_0545 at 632.844 kb on + strand, within PP_0545 at 632.845 kb on - strand, within PP_0545 at 632.996 kb on - strand, within PP_0545 at 633.281 kb on + strand, within PP_0545 at 633.281 kb on + strand, within PP_0545 at 633.281 kb on + strand, within PP_0545 at 633.282 kb on - strand, within PP_0545 at 633.326 kb on + strand at 633.356 kb on + strand at 633.356 kb on + strand at 633.356 kb on + strand at 633.357 kb on - strand at 633.357 kb on - strand at 633.376 kb on + strand at 633.377 kb on - strand at 633.377 kb on - strand at 633.377 kb on - strand at 633.377 kb on - strand at 633.377 kb on - strand at 633.385 kb on + strand at 633.434 kb on - strand at 633.456 kb on + strand at 633.456 kb on + strand at 633.675 kb on - strand at 633.689 kb on - strand at 633.736 kb on + strand at 633.815 kb on + strand at 633.816 kb on - strand at 634.252 kb on - strand, within PP_0546 at 634.319 kb on + strand, within PP_0546 at 634.319 kb on + strand, within PP_0546 at 634.320 kb on - strand, within PP_0546 at 634.320 kb on - strand, within PP_0546 at 634.470 kb on + strand, within PP_0546 at 634.864 kb on + strand, within PP_0546 at 634.865 kb on - strand, within PP_0546 at 634.865 kb on - strand, within PP_0546 at 635.000 kb on + strand, within PP_0546 at 635.000 kb on - strand, within PP_0546 at 635.104 kb on + strand, within PP_0546 at 635.159 kb on + strand, within PP_0546 at 635.159 kb on + strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.160 kb on - strand, within PP_0546 at 635.162 kb on + strand, within PP_0546 at 635.268 kb on + strand, within PP_0546 at 635.269 kb on - strand, within PP_0546 at 635.269 kb on - strand, within PP_0546 at 635.269 kb on - strand, within PP_0546 at 635.308 kb on + strand, within PP_0546 at 635.383 kb on + strand, within PP_0546 at 635.383 kb on + strand, within PP_0546 at 635.662 kb on + strand at 635.663 kb on - strand at 635.663 kb on - strand at 635.696 kb on - strand at 635.782 kb on + strand at 635.782 kb on + strand at 635.782 kb on + strand at 635.879 kb on + strand at 636.014 kb on + strand, within PP_0547 at 636.038 kb on + strand, within PP_0547 at 636.038 kb on + strand, within PP_0547 at 636.038 kb on + strand, within PP_0547 at 636.657 kb on + strand, within PP_0547 at 636.657 kb on + strand, within PP_0547 at 636.657 kb on + strand, within PP_0547
Per-strain Table
Position Strand Gene LocusTag Fraction ferulate (C); with MOPS remove 632,717 + PP_0545 0.52 -1.1 632,785 + PP_0545 0.57 +1.8 632,796 + PP_0545 0.58 -2.0 632,797 - PP_0545 0.58 -0.9 632,844 + PP_0545 0.61 -3.3 632,845 - PP_0545 0.61 -1.4 632,996 - PP_0545 0.71 -1.3 633,281 + PP_0545 0.89 +0.8 633,281 + PP_0545 0.89 -2.2 633,281 + PP_0545 0.89 +1.1 633,282 - PP_0545 0.90 +0.4 633,326 + +0.5 633,356 + -0.6 633,356 + +1.2 633,356 + -2.1 633,357 - +1.6 633,357 - -2.8 633,376 + +2.1 633,377 - -2.2 633,377 - +1.0 633,377 - -0.8 633,377 - -2.4 633,377 - +0.8 633,385 + -0.1 633,434 - -0.7 633,456 + -0.0 633,456 + -0.3 633,675 - +1.5 633,689 - +1.2 633,736 + -0.6 633,815 + -0.8 633,816 - +1.6 634,252 - PP_0546 0.28 -1.9 634,319 + PP_0546 0.31 +1.5 634,319 + PP_0546 0.31 -1.1 634,320 - PP_0546 0.31 -1.2 634,320 - PP_0546 0.31 +1.6 634,470 + PP_0546 0.38 -0.9 634,864 + PP_0546 0.58 +0.0 634,865 - PP_0546 0.58 +1.6 634,865 - PP_0546 0.58 +0.3 635,000 + PP_0546 0.65 +0.1 635,000 - PP_0546 0.65 +0.2 635,104 + PP_0546 0.70 +1.5 635,159 + PP_0546 0.73 -0.6 635,159 + PP_0546 0.73 +0.2 635,160 - PP_0546 0.73 -1.2 635,160 - PP_0546 0.73 +1.4 635,160 - PP_0546 0.73 -1.0 635,160 - PP_0546 0.73 -0.5 635,160 - PP_0546 0.73 +0.7 635,160 - PP_0546 0.73 +2.0 635,162 + PP_0546 0.73 -1.9 635,268 + PP_0546 0.78 +2.0 635,269 - PP_0546 0.78 +1.2 635,269 - PP_0546 0.78 +3.0 635,269 - PP_0546 0.78 +1.6 635,308 + PP_0546 0.80 -3.4 635,383 + PP_0546 0.84 +1.4 635,383 + PP_0546 0.84 -0.6 635,662 + -0.7 635,663 - -0.2 635,663 - +0.5 635,696 - -1.8 635,782 + -1.8 635,782 + -1.9 635,782 + -3.7 635,879 + -3.3 636,014 + PP_0547 0.18 -0.8 636,038 + PP_0547 0.19 -2.0 636,038 + PP_0547 0.19 -3.2 636,038 + PP_0547 0.19 -1.8 636,657 + PP_0547 0.65 -3.0 636,657 + PP_0547 0.65 -1.6 636,657 + PP_0547 0.65 -3.1
Or see this region's nucleotide sequence