Experiment: M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioF and bioC overlap by 14 nucleotides bioC and bioD overlap by 8 nucleotides bioD and uvrB are separated by 578 nucleotides
b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758
bioF
b0777: bioC - predicted methltransferase, enzyme of biotin synthesis (NCBI), at 810,745 to 811,500
bioC
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
b0779: uvrB - excinuclease ABC subunit B (NCBI), at 812,749 to 814,770
uvrB
Position (kb) 811
812
813 Strain fitness (log2 ratio) -2
-1
0
1 at 810.673 kb on + strand at 810.673 kb on - strand at 810.729 kb on + strand at 810.791 kb on + strand at 810.833 kb on + strand, within bioC at 810.971 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.366 kb on - strand, within bioC at 811.423 kb on + strand, within bioC at 811.423 kb on + strand, within bioC at 811.431 kb on - strand at 811.446 kb on - strand at 811.446 kb on - strand at 811.464 kb on - strand at 811.559 kb on + strand at 811.672 kb on + strand, within bioD at 811.720 kb on - strand, within bioD at 811.757 kb on + strand, within bioD at 812.000 kb on + strand, within bioD at 812.005 kb on - strand, within bioD at 812.005 kb on - strand, within bioD at 812.051 kb on + strand, within bioD at 812.051 kb on + strand, within bioD at 812.061 kb on - strand, within bioD at 812.295 kb on - strand at 812.353 kb on - strand at 812.834 kb on + strand at 812.957 kb on + strand, within uvrB at 812.967 kb on - strand, within uvrB at 813.039 kb on + strand, within uvrB at 813.050 kb on + strand, within uvrB at 813.055 kb on - strand, within uvrB at 813.106 kb on - strand, within uvrB at 813.106 kb on - strand, within uvrB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin remove 810,673 + -0.9 810,673 - -1.2 810,729 + -0.3 810,791 + -1.1 810,833 + bioC b0777 0.12 -1.9 810,971 + bioC b0777 0.30 -0.9 811,122 + bioC b0777 0.50 -1.0 811,122 + bioC b0777 0.50 -0.2 811,122 + bioC b0777 0.50 -1.1 811,122 + bioC b0777 0.50 -0.5 811,326 + bioC b0777 0.77 -2.1 811,326 + bioC b0777 0.77 -0.9 811,326 + bioC b0777 0.77 -0.5 811,366 - bioC b0777 0.82 -0.5 811,423 + bioC b0777 0.90 -0.3 811,423 + bioC b0777 0.90 -0.8 811,431 - -1.1 811,446 - -0.7 811,446 - -1.9 811,464 - -0.1 811,559 + -1.6 811,672 + bioD b0778 0.26 -1.9 811,720 - bioD b0778 0.33 -0.9 811,757 + bioD b0778 0.39 -0.8 812,000 + bioD b0778 0.75 -1.5 812,005 - bioD b0778 0.76 -0.9 812,005 - bioD b0778 0.76 -2.1 812,051 + bioD b0778 0.82 -1.3 812,051 + bioD b0778 0.82 -1.8 812,061 - bioD b0778 0.84 -2.6 812,295 - +0.2 812,353 - -0.8 812,834 + +0.6 812,957 + uvrB b0779 0.10 +0.1 812,967 - uvrB b0779 0.11 -0.2 813,039 + uvrB b0779 0.14 -0.3 813,050 + uvrB b0779 0.15 -0.5 813,055 - uvrB b0779 0.15 -0.0 813,106 - uvrB b0779 0.18 +0.1 813,106 - uvrB b0779 0.18 -0.6
Or see this region's nucleotide sequence