Experiment: M9 Glucose 0.2%, Thauer's vit mix without biotin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uhpT and uhpC are separated by 137 nucleotides uhpC and uhpB are separated by 9 nucleotides
b3666: uhpT - sugar phosphate antiporter (NCBI), at 3,843,799 to 3,845,190
uhpT
b3667: uhpC - regulator of uhpT (VIMSS), at 3,845,328 to 3,846,647
uhpC
b3668: uhpB - sensor histidine protein kinase phosphorylates UhpA (VIMSS), at 3,846,657 to 3,848,159
uhpB
Position (kb)
3845
3846
3847 Strain fitness (log2 ratio)
-1
0
1 at 3844.336 kb on + strand, within uhpT at 3844.336 kb on + strand, within uhpT at 3844.351 kb on + strand, within uhpT at 3844.366 kb on + strand, within uhpT at 3844.366 kb on - strand, within uhpT at 3844.381 kb on - strand, within uhpT at 3844.381 kb on - strand, within uhpT at 3844.404 kb on - strand, within uhpT at 3844.483 kb on - strand, within uhpT at 3844.488 kb on - strand, within uhpT at 3844.495 kb on - strand, within uhpT at 3844.495 kb on - strand, within uhpT at 3844.509 kb on + strand, within uhpT at 3844.512 kb on - strand, within uhpT at 3844.653 kb on + strand, within uhpT at 3844.653 kb on + strand, within uhpT at 3844.653 kb on + strand, within uhpT at 3844.744 kb on + strand, within uhpT at 3844.744 kb on + strand, within uhpT at 3844.819 kb on - strand, within uhpT at 3844.848 kb on - strand, within uhpT at 3844.848 kb on - strand, within uhpT at 3844.853 kb on - strand, within uhpT at 3844.863 kb on + strand, within uhpT at 3844.877 kb on - strand, within uhpT at 3844.877 kb on - strand, within uhpT at 3844.977 kb on - strand, within uhpT at 3844.997 kb on + strand, within uhpT at 3845.041 kb on + strand, within uhpT at 3845.069 kb on - strand at 3845.149 kb on + strand at 3845.187 kb on + strand at 3845.342 kb on + strand at 3845.350 kb on - strand at 3845.352 kb on - strand at 3845.352 kb on - strand at 3845.440 kb on + strand at 3845.477 kb on + strand, within uhpC at 3845.532 kb on + strand, within uhpC at 3845.620 kb on - strand, within uhpC at 3845.620 kb on - strand, within uhpC at 3845.636 kb on + strand, within uhpC at 3845.644 kb on - strand, within uhpC at 3845.709 kb on - strand, within uhpC at 3845.709 kb on - strand, within uhpC at 3845.719 kb on - strand, within uhpC at 3845.776 kb on + strand, within uhpC at 3845.809 kb on + strand, within uhpC at 3845.809 kb on + strand, within uhpC at 3845.855 kb on + strand, within uhpC at 3845.857 kb on + strand, within uhpC at 3845.865 kb on - strand, within uhpC at 3845.865 kb on - strand, within uhpC at 3845.865 kb on - strand, within uhpC at 3845.950 kb on - strand, within uhpC at 3845.968 kb on + strand, within uhpC at 3845.991 kb on + strand, within uhpC at 3846.103 kb on + strand, within uhpC at 3846.133 kb on + strand, within uhpC at 3846.171 kb on + strand, within uhpC at 3846.171 kb on + strand, within uhpC at 3846.176 kb on - strand, within uhpC at 3846.176 kb on - strand, within uhpC at 3846.236 kb on - strand, within uhpC at 3846.271 kb on + strand, within uhpC at 3846.271 kb on + strand, within uhpC at 3846.284 kb on - strand, within uhpC at 3846.344 kb on + strand, within uhpC at 3846.344 kb on + strand, within uhpC at 3846.347 kb on - strand, within uhpC at 3846.410 kb on - strand, within uhpC at 3846.411 kb on + strand, within uhpC at 3846.418 kb on - strand, within uhpC at 3846.419 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.554 kb on - strand at 3846.604 kb on + strand at 3846.655 kb on + strand at 3846.674 kb on - strand at 3846.674 kb on - strand at 3846.685 kb on - strand at 3846.689 kb on + strand at 3846.815 kb on - strand, within uhpB at 3846.815 kb on - strand, within uhpB at 3846.965 kb on + strand, within uhpB at 3846.965 kb on + strand, within uhpB at 3846.983 kb on - strand, within uhpB at 3846.983 kb on - strand, within uhpB at 3847.125 kb on + strand, within uhpB at 3847.125 kb on + strand, within uhpB at 3847.172 kb on - strand at 3847.174 kb on + strand, within uhpB at 3847.190 kb on + strand, within uhpB at 3847.203 kb on + strand, within uhpB at 3847.243 kb on - strand, within uhpB at 3847.253 kb on - strand, within uhpB at 3847.264 kb on - strand, within uhpB at 3847.327 kb on + strand, within uhpB at 3847.370 kb on + strand, within uhpB at 3847.422 kb on - strand, within uhpB at 3847.422 kb on - strand, within uhpB at 3847.464 kb on + strand, within uhpB at 3847.480 kb on + strand, within uhpB at 3847.488 kb on - strand, within uhpB at 3847.494 kb on - strand, within uhpB at 3847.516 kb on + strand, within uhpB at 3847.593 kb on + strand, within uhpB at 3847.593 kb on + strand, within uhpB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Glucose 0.2%, Thauer's vit mix without biotin remove 3,844,336 + uhpT b3666 0.39 +0.3 3,844,336 + uhpT b3666 0.39 +0.5 3,844,351 + uhpT b3666 0.40 +0.1 3,844,366 + uhpT b3666 0.41 -0.3 3,844,366 - uhpT b3666 0.41 -0.1 3,844,381 - uhpT b3666 0.42 +0.5 3,844,381 - uhpT b3666 0.42 -0.5 3,844,404 - uhpT b3666 0.43 +0.1 3,844,483 - uhpT b3666 0.49 +0.3 3,844,488 - uhpT b3666 0.49 +0.0 3,844,495 - uhpT b3666 0.50 +0.0 3,844,495 - uhpT b3666 0.50 +0.5 3,844,509 + uhpT b3666 0.51 -0.0 3,844,512 - uhpT b3666 0.51 +0.1 3,844,653 + uhpT b3666 0.61 -0.1 3,844,653 + uhpT b3666 0.61 +0.2 3,844,653 + uhpT b3666 0.61 +0.7 3,844,744 + uhpT b3666 0.68 -0.3 3,844,744 + uhpT b3666 0.68 +0.3 3,844,819 - uhpT b3666 0.73 +0.3 3,844,848 - uhpT b3666 0.75 +0.2 3,844,848 - uhpT b3666 0.75 -0.1 3,844,853 - uhpT b3666 0.76 -0.1 3,844,863 + uhpT b3666 0.76 +0.2 3,844,877 - uhpT b3666 0.77 +0.2 3,844,877 - uhpT b3666 0.77 -0.6 3,844,977 - uhpT b3666 0.85 -0.7 3,844,997 + uhpT b3666 0.86 +0.3 3,845,041 + uhpT b3666 0.89 -0.3 3,845,069 - +0.1 3,845,149 + +0.3 3,845,187 + -0.6 3,845,342 + +0.2 3,845,350 - +0.3 3,845,352 - -0.1 3,845,352 - +1.5 3,845,440 + +0.1 3,845,477 + uhpC b3667 0.11 +0.6 3,845,532 + uhpC b3667 0.15 +0.7 3,845,620 - uhpC b3667 0.22 -0.2 3,845,620 - uhpC b3667 0.22 -0.0 3,845,636 + uhpC b3667 0.23 -0.1 3,845,644 - uhpC b3667 0.24 +0.1 3,845,709 - uhpC b3667 0.29 -0.0 3,845,709 - uhpC b3667 0.29 +1.1 3,845,719 - uhpC b3667 0.30 -0.8 3,845,776 + uhpC b3667 0.34 +0.1 3,845,809 + uhpC b3667 0.36 -0.1 3,845,809 + uhpC b3667 0.36 +0.4 3,845,855 + uhpC b3667 0.40 +0.4 3,845,857 + uhpC b3667 0.40 +0.1 3,845,865 - uhpC b3667 0.41 +0.5 3,845,865 - uhpC b3667 0.41 +0.5 3,845,865 - uhpC b3667 0.41 +0.1 3,845,950 - uhpC b3667 0.47 -0.3 3,845,968 + uhpC b3667 0.48 +0.3 3,845,991 + uhpC b3667 0.50 -0.3 3,846,103 + uhpC b3667 0.59 +0.2 3,846,133 + uhpC b3667 0.61 +0.2 3,846,171 + uhpC b3667 0.64 -0.1 3,846,171 + uhpC b3667 0.64 +0.2 3,846,176 - uhpC b3667 0.64 -0.6 3,846,176 - uhpC b3667 0.64 -1.1 3,846,236 - uhpC b3667 0.69 -0.1 3,846,271 + uhpC b3667 0.71 +0.1 3,846,271 + uhpC b3667 0.71 +0.4 3,846,284 - uhpC b3667 0.72 -0.2 3,846,344 + uhpC b3667 0.77 -0.0 3,846,344 + uhpC b3667 0.77 +0.0 3,846,347 - uhpC b3667 0.77 +0.5 3,846,410 - uhpC b3667 0.82 -0.9 3,846,411 + uhpC b3667 0.82 -0.7 3,846,418 - uhpC b3667 0.83 -0.1 3,846,419 - uhpC b3667 0.83 -0.0 3,846,429 - uhpC b3667 0.83 +0.1 3,846,429 - uhpC b3667 0.83 +0.5 3,846,429 - uhpC b3667 0.83 +0.2 3,846,429 - uhpC b3667 0.83 +0.4 3,846,554 - -0.6 3,846,604 + +0.4 3,846,655 + -0.3 3,846,674 - +0.0 3,846,674 - +0.3 3,846,685 - +0.2 3,846,689 + -0.0 3,846,815 - uhpB b3668 0.11 +0.3 3,846,815 - uhpB b3668 0.11 +0.4 3,846,965 + uhpB b3668 0.20 -0.3 3,846,965 + uhpB b3668 0.20 -0.7 3,846,983 - uhpB b3668 0.22 -0.3 3,846,983 - uhpB b3668 0.22 -0.6 3,847,125 + uhpB b3668 0.31 +0.2 3,847,125 + uhpB b3668 0.31 -0.0 3,847,172 - +0.6 3,847,174 + uhpB b3668 0.34 +0.1 3,847,190 + uhpB b3668 0.35 +0.0 3,847,203 + uhpB b3668 0.36 +0.3 3,847,243 - uhpB b3668 0.39 -0.1 3,847,253 - uhpB b3668 0.40 -0.2 3,847,264 - uhpB b3668 0.40 +0.6 3,847,327 + uhpB b3668 0.45 +0.2 3,847,370 + uhpB b3668 0.47 -0.3 3,847,422 - uhpB b3668 0.51 -0.8 3,847,422 - uhpB b3668 0.51 +0.1 3,847,464 + uhpB b3668 0.54 +0.0 3,847,480 + uhpB b3668 0.55 +0.0 3,847,488 - uhpB b3668 0.55 +0.2 3,847,494 - uhpB b3668 0.56 +0.8 3,847,516 + uhpB b3668 0.57 +0.1 3,847,593 + uhpB b3668 0.62 +0.2 3,847,593 + uhpB b3668 0.62 +0.2
Or see this region's nucleotide sequence