Experiment: M9 Glucose 0.2%, Thauer's vit mix without biotin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaiC and proC are separated by 16 nucleotides proC and yaiI are separated by 119 nucleotides yaiI and aroL are separated by 182 nucleotides
b0385: yaiC - predicted diguanylate cyclase (NCBI), at 402,927 to 404,042
yaiC
b0386: proC - pyrroline-5-carboxylate reductase (NCBI), at 404,059 to 404,868
proC
b0387: yaiI - orf, hypothetical protein (VIMSS), at 404,988 to 405,446
yaiI
b0388: aroL - shikimate kinase II (NCBI), at 405,629 to 406,153
aroL
Position (kb) 404
405 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 403.063 kb on + strand, within yaiC at 403.130 kb on + strand, within yaiC at 403.291 kb on + strand, within yaiC at 403.558 kb on - strand, within yaiC at 403.558 kb on - strand, within yaiC at 403.574 kb on - strand, within yaiC at 403.574 kb on - strand, within yaiC at 403.600 kb on - strand, within yaiC at 403.600 kb on - strand, within yaiC at 403.892 kb on + strand, within yaiC at 403.892 kb on + strand, within yaiC at 403.970 kb on + strand at 403.999 kb on + strand at 404.002 kb on - strand at 404.123 kb on - strand at 404.154 kb on + strand, within proC at 404.177 kb on + strand, within proC at 404.177 kb on + strand, within proC at 404.182 kb on + strand, within proC at 404.182 kb on + strand, within proC at 404.188 kb on + strand, within proC at 404.270 kb on + strand, within proC at 404.343 kb on + strand, within proC at 404.427 kb on + strand, within proC at 404.514 kb on - strand, within proC at 404.539 kb on + strand, within proC at 404.566 kb on + strand, within proC at 404.572 kb on - strand, within proC at 404.588 kb on - strand, within proC at 404.588 kb on - strand, within proC at 404.588 kb on - strand, within proC at 404.717 kb on + strand, within proC at 404.717 kb on + strand, within proC at 404.778 kb on + strand, within proC at 405.063 kb on - strand, within yaiI at 405.237 kb on + strand, within yaiI at 405.352 kb on - strand, within yaiI at 405.370 kb on - strand, within yaiI at 405.370 kb on - strand, within yaiI at 405.370 kb on - strand, within yaiI at 405.584 kb on - strand at 405.584 kb on - strand at 405.675 kb on - strand at 405.867 kb on - strand, within aroL
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Glucose 0.2%, Thauer's vit mix without biotin remove 403,063 + yaiC b0385 0.12 +0.4 403,130 + yaiC b0385 0.18 +0.3 403,291 + yaiC b0385 0.33 -0.2 403,558 - yaiC b0385 0.57 +0.7 403,558 - yaiC b0385 0.57 +0.3 403,574 - yaiC b0385 0.58 -0.5 403,574 - yaiC b0385 0.58 -0.3 403,600 - yaiC b0385 0.60 +0.3 403,600 - yaiC b0385 0.60 +0.2 403,892 + yaiC b0385 0.86 +0.4 403,892 + yaiC b0385 0.86 +0.7 403,970 + +0.4 403,999 + -0.0 404,002 - -1.1 404,123 - -4.7 404,154 + proC b0386 0.12 -4.2 404,177 + proC b0386 0.15 -3.7 404,177 + proC b0386 0.15 -4.9 404,182 + proC b0386 0.15 -4.5 404,182 + proC b0386 0.15 -3.7 404,188 + proC b0386 0.16 -2.4 404,270 + proC b0386 0.26 -4.1 404,343 + proC b0386 0.35 -3.9 404,427 + proC b0386 0.45 -3.8 404,514 - proC b0386 0.56 -3.8 404,539 + proC b0386 0.59 -4.5 404,566 + proC b0386 0.63 -4.0 404,572 - proC b0386 0.63 -6.0 404,588 - proC b0386 0.65 -3.9 404,588 - proC b0386 0.65 -4.4 404,588 - proC b0386 0.65 -2.6 404,717 + proC b0386 0.81 -4.0 404,717 + proC b0386 0.81 -3.1 404,778 + proC b0386 0.89 -2.8 405,063 - yaiI b0387 0.16 +0.3 405,237 + yaiI b0387 0.54 -0.2 405,352 - yaiI b0387 0.79 -0.1 405,370 - yaiI b0387 0.83 +0.4 405,370 - yaiI b0387 0.83 +0.1 405,370 - yaiI b0387 0.83 +0.1 405,584 - -0.1 405,584 - +0.7 405,675 - +0.2 405,867 - aroL b0388 0.45 +0.2
Or see this region's nucleotide sequence