Strain Fitness in Escherichia coli BW25113 around b3744

Experiment: M9 Glucose 0.2%, Thauer's vit mix without biotin

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 Glucose 0.2%, Thauer's vit mix without biotin
3,924,205	+	mioC	b3742	0.38	-0.1
3,924,206	+	mioC	b3742	0.39	-0.5
3,924,206	+	mioC	b3742	0.39	+0.1
3,924,307	+	mioC	b3742	0.61	+0.1
3,924,323	-	mioC	b3742	0.65	-0.4
3,924,330	-	mioC	b3742	0.66	+0.0
3,924,447	-				-1.3
3,924,458	+				-0.2
3,924,458	+				-0.3
3,924,465	+				+0.2
3,924,471	-				-0.2
3,924,507	+				+0.1
3,924,507	+				+0.2
3,924,507	+				-0.1
3,924,515	+				-0.2
3,924,515	-				+0.3
3,924,515	-				-0.1
3,924,515	-				+0.0
3,924,525	-				-0.3
3,924,530	+				+0.7
3,924,664	+	asnC	b3743	0.21	+0.6
3,924,668	-	asnC	b3743	0.22	+0.3
3,924,668	-	asnC	b3743	0.22	+0.2
3,924,672	-	asnC	b3743	0.23	-0.2
3,924,696	+	asnC	b3743	0.28	-0.2
3,924,696	+	asnC	b3743	0.28	-0.2
3,924,708	-	asnC	b3743	0.31	+0.1
3,924,720	-	asnC	b3743	0.33	+0.1
3,924,720	-	asnC	b3743	0.33	+0.1
3,924,720	-	asnC	b3743	0.33	-0.2
3,924,722	+	asnC	b3743	0.34	+0.1
3,924,730	-	asnC	b3743	0.35	+0.2
3,924,730	-	asnC	b3743	0.35	+0.3
3,924,742	-	asnC	b3743	0.38	-0.2
3,924,742	-	asnC	b3743	0.38	-0.8
3,924,793	-	asnC	b3743	0.49	-0.3
3,924,796	+	asnC	b3743	0.50	+0.0
3,924,823	-	asnC	b3743	0.56	+0.5
3,924,840	+	asnC	b3743	0.59	+0.4
3,924,850	-	asnC	b3743	0.61	+0.4
3,924,852	+	asnC	b3743	0.62	-0.5
3,924,860	-	asnC	b3743	0.64	-0.1
3,924,904	+	asnC	b3743	0.73	-0.5
3,924,911	+	asnC	b3743	0.75	+0.3
3,924,911	+	asnC	b3743	0.75	-0.7
3,924,923	-	asnC	b3743	0.77	+0.4
3,924,965	+	asnC	b3743	0.86	+0.0
3,924,990	-				+0.0
3,925,206	+				+0.2
3,925,206	+				+0.0
3,925,210	+				+0.5
3,925,254	+				-0.1
3,925,301	-	asnA	b3744	0.12	-0.0
3,925,301	-	asnA	b3744	0.12	+0.2
3,925,301	-	asnA	b3744	0.12	-0.6
3,925,305	-	asnA	b3744	0.13	+0.9
3,925,312	+	asnA	b3744	0.13	+0.3
3,925,312	+	asnA	b3744	0.13	+0.1
3,925,312	+	asnA	b3744	0.13	+0.1
3,925,320	-	asnA	b3744	0.14	+0.3
3,925,320	-	asnA	b3744	0.14	+0.4
3,925,320	-	asnA	b3744	0.14	+0.2
3,925,348	-	asnA	b3744	0.17	-0.7
3,925,358	+	asnA	b3744	0.18	+1.0
3,925,388	-	asnA	b3744	0.21	-0.4
3,925,411	+	asnA	b3744	0.23	-0.2
3,925,417	-	asnA	b3744	0.24	+0.1
3,925,443	+	asnA	b3744	0.27	-0.1
3,925,443	+	asnA	b3744	0.27	+0.7
3,925,458	-	asnA	b3744	0.28	+0.6
3,925,462	-	asnA	b3744	0.29	+0.8
3,925,470	-	asnA	b3744	0.29	-0.1
3,925,470	-	asnA	b3744	0.29	+0.7
3,925,489	-	asnA	b3744	0.31	+0.6
3,925,520	+	asnA	b3744	0.34	-0.3
3,925,536	-	asnA	b3744	0.36	+0.3
3,925,543	-	asnA	b3744	0.37	-0.0
3,925,592	-	asnA	b3744	0.42	+0.3
3,925,592	-	asnA	b3744	0.42	+0.5
3,925,606	+	asnA	b3744	0.43	+0.2
3,925,607	-	asnA	b3744	0.43	-0.1
3,925,632	+	asnA	b3744	0.46	-0.0
3,925,632	+	asnA	b3744	0.46	-0.2
3,925,637	-	asnA	b3744	0.46	-0.2
3,925,644	+	asnA	b3744	0.47	+0.5
3,925,675	+	asnA	b3744	0.50	-0.1
3,925,675	+	asnA	b3744	0.50	+0.5
3,925,684	-	asnA	b3744	0.51	+0.1
3,925,686	+	asnA	b3744	0.51	+0.4
3,925,692	-	asnA	b3744	0.52	+0.4
3,925,693	-	asnA	b3744	0.52	+0.5
3,925,789	+	asnA	b3744	0.62	+0.2
3,925,797	-	asnA	b3744	0.62	+0.5
3,925,808	-	asnA	b3744	0.63	-0.1
3,925,808	-	asnA	b3744	0.63	+0.3
3,925,823	+	asnA	b3744	0.65	+0.1
3,925,842	-	asnA	b3744	0.67	+0.4
3,925,845	-				+0.8
3,925,845	-	asnA	b3744	0.67	-0.0
3,925,848	+	asnA	b3744	0.67	-0.6
3,925,848	+	asnA	b3744	0.67	+0.5
3,925,848	+	asnA	b3744	0.67	+0.2
3,925,848	+	asnA	b3744	0.67	+0.2
3,925,853	+	asnA	b3744	0.68	+0.3
3,925,853	+	asnA	b3744	0.68	+0.2
3,925,860	+	asnA	b3744	0.69	+0.7
3,925,868	+	asnA	b3744	0.69	+0.3
3,925,868	+	asnA	b3744	0.69	+0.4
3,925,941	+	asnA	b3744	0.77	+0.6
3,925,954	-	asnA	b3744	0.78	+0.1
3,925,986	-	asnA	b3744	0.81	-0.2
3,926,000	+	asnA	b3744	0.83	+0.1
3,926,000	+	asnA	b3744	0.83	+0.7
3,926,000	+	asnA	b3744	0.83	+0.3
3,926,005	+	asnA	b3744	0.83	+0.5
3,926,010	+	asnA	b3744	0.84	+0.5
3,926,058	+	asnA	b3744	0.89	-0.3
3,926,076	+				+0.3
3,926,080	-				+0.0
3,926,080	-				+0.5
3,926,093	-				+1.0
3,926,125	+				+0.3
3,926,127	-				+0.4
3,926,133	-				+0.6
3,926,135	+				+0.2
3,926,135	+				+1.0
3,926,148	+				+0.6
3,926,148	+				+0.9
3,926,165	-				+0.1
3,926,165	-				+0.5
3,926,261	-				+0.4
3,926,295	+				+0.4
3,926,308	-				+0.2
3,926,308	-				+0.4
3,926,308	-				+0.5
3,926,398	+	yieM	b3745	0.15	+0.1
3,926,423	-	yieM	b3745	0.17	+0.2
3,926,496	+	yieM	b3745	0.22	+0.2
3,926,506	-	yieM	b3745	0.23	-0.0
3,926,513	+	yieM	b3745	0.23	+0.5
3,926,554	-	yieM	b3745	0.26	+0.6
3,926,583	+	yieM	b3745	0.28	+0.6
3,926,605	+	yieM	b3745	0.30	+0.5
3,926,613	-	yieM	b3745	0.30	+0.1
3,926,615	+	yieM	b3745	0.30	+0.2
3,926,656	+	yieM	b3745	0.33	+0.3
3,926,733	-	yieM	b3745	0.38	+0.1
3,926,735	-	yieM	b3745	0.39	+0.4
3,926,739	+	yieM	b3745	0.39	+0.2
3,926,752	-	yieM	b3745	0.40	-0.7
3,926,752	-	yieM	b3745	0.40	+0.5
3,926,752	-	yieM	b3745	0.40	-0.0
3,926,752	-	yieM	b3745	0.40	+0.3
3,926,752	-	yieM	b3745	0.40	-0.4
3,926,752	-	yieM	b3745	0.40	-0.1
3,926,752	-	yieM	b3745	0.40	-0.2
3,926,801	-	yieM	b3745	0.43	+0.2
3,926,807	+	yieM	b3745	0.44	-0.2
3,926,807	+	yieM	b3745	0.44	-0.1
3,926,839	-	yieM	b3745	0.46	+0.4
3,926,874	-	yieM	b3745	0.48	+0.0
3,926,874	-	yieM	b3745	0.48	+0.4
3,926,927	-	yieM	b3745	0.52	+0.0
3,926,942	+	yieM	b3745	0.53	-0.5
3,926,942	+	yieM	b3745	0.53	-0.3
3,927,009	+	yieM	b3745	0.57	+0.2
3,927,024	+	yieM	b3745	0.58	+0.1
3,927,024	+	yieM	b3745	0.58	-0.3
3,927,024	+	yieM	b3745	0.58	+0.1
3,927,070	+	yieM	b3745	0.62	+0.1
3,927,070	+	yieM	b3745	0.62	+0.2
3,927,071	+	yieM	b3745	0.62	+0.4
3,927,071	+	yieM	b3745	0.62	+0.3
3,927,104	-	yieM	b3745	0.64	+0.5
3,927,104	-	yieM	b3745	0.64	+0.7
3,927,129	+	yieM	b3745	0.66	+0.4
3,927,154	-	yieM	b3745	0.67	-0.4
3,927,157	+	yieM	b3745	0.68	-0.7
3,927,157	+	yieM	b3745	0.68	+0.2
3,927,159	+	yieM	b3745	0.68	+0.7
3,927,167	-	yieM	b3745	0.68	-0.2