Strain Fitness in Escherichia coli BW25113 around b1747

Experiment: M9 Glucose 0.2%, Thauer's vit mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntastD and astA overlap by 4 nucleotidesastA and astC overlap by 4 nucleotides b1746: astD - succinylglutamic semialdehyde dehydrogenase (NCBI), at 1,826,280 to 1,827,758 astD b1747: astA - arginine succinyltransferase (NCBI), at 1,827,755 to 1,828,789 astA b1748: astC - succinylornithine transaminase, PLP-dependent (NCBI), at 1,828,786 to 1,830,006 astC Position (kb) 1827 1828 1829Strain fitness (log2 ratio) -1 0 1at 1826.786 kb on + strand, within astDat 1826.848 kb on - strand, within astDat 1826.848 kb on - strand, within astDat 1826.870 kb on - strand, within astDat 1826.949 kb on - strand, within astDat 1826.949 kb on - strand, within astDat 1827.115 kb on + strand, within astDat 1827.262 kb on - strand, within astDat 1827.262 kb on - strand, within astDat 1827.350 kb on - strand, within astDat 1827.814 kb on + strandat 1827.814 kb on + strandat 1827.870 kb on - strand, within astAat 1827.875 kb on + strand, within astAat 1828.010 kb on - strand, within astAat 1828.010 kb on - strand, within astAat 1828.024 kb on - strand, within astAat 1828.076 kb on - strand, within astAat 1828.076 kb on - strand, within astAat 1828.129 kb on - strand, within astAat 1828.236 kb on + strand, within astAat 1828.424 kb on - strand, within astAat 1828.629 kb on + strand, within astAat 1828.765 kb on + strandat 1829.079 kb on + strand, within astCat 1829.172 kb on + strand, within astCat 1829.172 kb on + strand, within astCat 1829.304 kb on - strand, within astCat 1829.304 kb on - strand, within astCat 1829.492 kb on - strand, within astCat 1829.492 kb on - strand, within astC

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Glucose 0.2%, Thauer's vit mix
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1,826,786 + astD b1746 0.34 -0.4
1,826,848 - astD b1746 0.38 +0.8
1,826,848 - astD b1746 0.38 -0.6
1,826,870 - astD b1746 0.40 -1.0
1,826,949 - astD b1746 0.45 +0.7
1,826,949 - astD b1746 0.45 +0.5
1,827,115 + astD b1746 0.56 -0.2
1,827,262 - astD b1746 0.66 +0.4
1,827,262 - astD b1746 0.66 +0.2
1,827,350 - astD b1746 0.72 +0.1
1,827,814 + +1.0
1,827,814 + +0.6
1,827,870 - astA b1747 0.11 +0.1
1,827,875 + astA b1747 0.12 +0.1
1,828,010 - astA b1747 0.25 -0.0
1,828,010 - astA b1747 0.25 -0.1
1,828,024 - astA b1747 0.26 +0.3
1,828,076 - astA b1747 0.31 -0.5
1,828,076 - astA b1747 0.31 +0.2
1,828,129 - astA b1747 0.36 -0.3
1,828,236 + astA b1747 0.46 -0.5
1,828,424 - astA b1747 0.65 +0.0
1,828,629 + astA b1747 0.84 +0.1
1,828,765 + -0.1
1,829,079 + astC b1748 0.24 +0.3
1,829,172 + astC b1748 0.32 +0.3
1,829,172 + astC b1748 0.32 +0.1
1,829,304 - astC b1748 0.42 +0.2
1,829,304 - astC b1748 0.42 +0.4
1,829,492 - astC b1748 0.58 +0.3
1,829,492 - astC b1748 0.58 +0.0

Or see this region's nucleotide sequence