Experiment: M9 Glucose 0.2%, Thauer's vit mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysA and cysW overlap by 11 nucleotides cysW and cysU overlap by 1 nucleotides cysU and cysP overlap by 1 nucleotides
b2422: cysA - sulfate/thiosulfate transporter subunit (NCBI), at 2,537,739 to 2,538,836
cysA
b2423: cysW - sulfate/thiosulfate transporter subunit (NCBI), at 2,538,826 to 2,539,701
cysW
b2424: cysU - sulfate/thiosulfate transporter subunit (NCBI), at 2,539,701 to 2,540,534
cysU
b2425: cysP - thiosulfate transporter subunit (NCBI), at 2,540,534 to 2,541,550
cysP
Position (kb)
2538
2539
2540 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2537.849 kb on + strand, within cysA at 2537.862 kb on - strand, within cysA at 2537.903 kb on - strand, within cysA at 2537.963 kb on - strand, within cysA at 2538.033 kb on + strand, within cysA at 2538.037 kb on - strand, within cysA at 2538.054 kb on - strand, within cysA at 2538.054 kb on - strand, within cysA at 2538.084 kb on - strand, within cysA at 2538.108 kb on - strand, within cysA at 2538.114 kb on + strand, within cysA at 2538.193 kb on + strand, within cysA at 2538.193 kb on + strand, within cysA at 2538.224 kb on + strand, within cysA at 2538.224 kb on + strand, within cysA at 2538.334 kb on - strand, within cysA at 2538.334 kb on - strand, within cysA at 2538.336 kb on - strand, within cysA at 2538.699 kb on + strand, within cysA at 2538.824 kb on + strand at 2538.824 kb on + strand at 2538.846 kb on - strand at 2538.846 kb on - strand at 2538.952 kb on + strand, within cysW at 2538.952 kb on + strand, within cysW at 2538.959 kb on + strand, within cysW at 2538.961 kb on + strand, within cysW at 2538.986 kb on + strand, within cysW at 2539.072 kb on + strand, within cysW at 2539.173 kb on + strand, within cysW at 2539.173 kb on + strand, within cysW at 2539.240 kb on + strand, within cysW at 2539.249 kb on - strand, within cysW at 2539.249 kb on - strand, within cysW at 2539.456 kb on + strand, within cysW at 2539.468 kb on - strand, within cysW at 2539.468 kb on - strand, within cysW at 2539.468 kb on - strand, within cysW at 2539.694 kb on - strand at 2540.062 kb on + strand, within cysU at 2540.110 kb on - strand, within cysU at 2540.110 kb on - strand, within cysU at 2540.129 kb on + strand, within cysU at 2540.492 kb on + strand at 2540.535 kb on + strand at 2540.563 kb on - strand at 2540.677 kb on - strand, within cysP at 2540.677 kb on - strand, within cysP at 2540.695 kb on - strand, within cysP
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Glucose 0.2%, Thauer's vit mix remove 2,537,849 + cysA b2422 0.10 -1.6 2,537,862 - cysA b2422 0.11 -2.1 2,537,903 - cysA b2422 0.15 -2.0 2,537,963 - cysA b2422 0.20 -1.3 2,538,033 + cysA b2422 0.27 -1.6 2,538,037 - cysA b2422 0.27 -2.6 2,538,054 - cysA b2422 0.29 -1.0 2,538,054 - cysA b2422 0.29 -1.5 2,538,084 - cysA b2422 0.31 -2.0 2,538,108 - cysA b2422 0.34 -2.3 2,538,114 + cysA b2422 0.34 -2.5 2,538,193 + cysA b2422 0.41 -1.8 2,538,193 + cysA b2422 0.41 -2.5 2,538,224 + cysA b2422 0.44 -2.0 2,538,224 + cysA b2422 0.44 -1.2 2,538,334 - cysA b2422 0.54 -2.2 2,538,334 - cysA b2422 0.54 -2.6 2,538,336 - cysA b2422 0.54 -2.1 2,538,699 + cysA b2422 0.87 -1.9 2,538,824 + -2.4 2,538,824 + -2.1 2,538,846 - -0.4 2,538,846 - +0.5 2,538,952 + cysW b2423 0.14 -2.0 2,538,952 + cysW b2423 0.14 -1.7 2,538,959 + cysW b2423 0.15 -0.4 2,538,961 + cysW b2423 0.15 -2.3 2,538,986 + cysW b2423 0.18 -2.3 2,539,072 + cysW b2423 0.28 -2.3 2,539,173 + cysW b2423 0.40 -2.6 2,539,173 + cysW b2423 0.40 -1.8 2,539,240 + cysW b2423 0.47 -1.5 2,539,249 - cysW b2423 0.48 -1.3 2,539,249 - cysW b2423 0.48 -3.2 2,539,456 + cysW b2423 0.72 -2.3 2,539,468 - cysW b2423 0.73 -2.4 2,539,468 - cysW b2423 0.73 -2.0 2,539,468 - cysW b2423 0.73 -1.5 2,539,694 - -2.1 2,540,062 + cysU b2424 0.43 -2.0 2,540,110 - cysU b2424 0.49 -2.4 2,540,110 - cysU b2424 0.49 -1.4 2,540,129 + cysU b2424 0.51 -2.4 2,540,492 + -0.8 2,540,535 + -1.6 2,540,563 - -1.3 2,540,677 - cysP b2425 0.14 +0.0 2,540,677 - cysP b2425 0.14 +0.0 2,540,695 - cysP b2425 0.16 -0.3
Or see this region's nucleotide sequence