Experiment: Phage 100.3 + bacteria
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1337 and Atu1338 are separated by 127 nucleotides Atu1338 and Atu1339 are separated by 113 nucleotides Atu1339 and Atu8016 are separated by 259 nucleotides Atu8016 and Atu1340 are separated by 245 nucleotides
Atu1337: Atu1337 - hypothetical protein, at 1,322,490 to 1,322,855
Atu1337
Atu1338: Atu1338 - 3-oxoacyl-(acyl carrier protein) reductase, at 1,322,983 to 1,323,687
Atu1338
Atu1339: Atu1339 - ribonuclease E, at 1,323,801 to 1,326,734
Atu1339
Atu8016: Atu8016 - hypothetical protein, at 1,326,994 to 1,327,134
Atu8016
Atu1340: Atu1340 - N-acetylmuramoyl-L-alanine amidase, at 1,327,380 to 1,328,639
Atu1340
Position (kb)
1323
1324
1325
1326
1327 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1322.942 kb on - strand at 1322.966 kb on + strand at 1323.099 kb on + strand, within Atu1338 at 1323.143 kb on + strand, within Atu1338 at 1323.153 kb on + strand, within Atu1338 at 1323.153 kb on + strand, within Atu1338 at 1323.222 kb on + strand, within Atu1338 at 1323.384 kb on + strand, within Atu1338 at 1323.631 kb on + strand at 1323.633 kb on + strand at 1323.639 kb on - strand at 1323.641 kb on - strand at 1323.641 kb on - strand at 1323.641 kb on - strand at 1323.653 kb on + strand at 1323.934 kb on - strand at 1324.051 kb on - strand at 1324.185 kb on + strand, within Atu1339 at 1324.191 kb on - strand, within Atu1339 at 1324.234 kb on + strand, within Atu1339 at 1324.479 kb on - strand, within Atu1339 at 1327.257 kb on + strand at 1327.280 kb on + strand at 1327.288 kb on + strand at 1327.289 kb on - strand at 1327.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phage 100.3 + bacteria remove 1,322,942 - -0.8 1,322,966 + -0.4 1,323,099 + Atu1338 0.16 -0.9 1,323,143 + Atu1338 0.23 -1.5 1,323,153 + Atu1338 0.24 -0.5 1,323,153 + Atu1338 0.24 -0.2 1,323,222 + Atu1338 0.34 +0.0 1,323,384 + Atu1338 0.57 +0.4 1,323,631 + -0.8 1,323,633 + -0.4 1,323,639 - +0.0 1,323,641 - +0.1 1,323,641 - -0.0 1,323,641 - +0.0 1,323,653 + -0.0 1,323,934 - -0.9 1,324,051 - -2.0 1,324,185 + Atu1339 0.13 -0.3 1,324,191 - Atu1339 0.13 -0.3 1,324,234 + Atu1339 0.15 -1.4 1,324,479 - Atu1339 0.23 -1.6 1,327,257 + -0.0 1,327,280 + +2.7 1,327,288 + -0.4 1,327,289 - +0.5 1,327,329 + -0.1
Or see this region's nucleotide sequence