Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16285 and PS417_16290 are separated by 800 nucleotides PS417_16290 and PS417_16295 are separated by 179 nucleotides PS417_16295 and PS417_16305 are separated by 398 nucleotides
PS417_16285: PS417_16285 - diguanylate cyclase, at 3,567,634 to 3,568,491
_16285
PS417_16290: PS417_16290 - hypothetical protein, at 3,569,292 to 3,569,675
_16290
PS417_16295: PS417_16295 - hypothetical protein, at 3,569,855 to 3,570,187
_16295
PS417_16305: PS417_16305 - tRNA-Ser, at 3,570,586 to 3,570,676
_16305
Position (kb)
3569
3570 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3568.325 kb on + strand, within PS417_16285 at 3568.325 kb on + strand, within PS417_16285 at 3568.326 kb on - strand, within PS417_16285 at 3568.338 kb on - strand, within PS417_16285 at 3568.431 kb on + strand at 3568.463 kb on - strand at 3568.463 kb on - strand at 3568.489 kb on + strand at 3568.489 kb on + strand at 3568.489 kb on + strand at 3568.518 kb on - strand at 3568.636 kb on + strand at 3568.637 kb on - strand at 3568.662 kb on + strand at 3568.663 kb on - strand at 3568.776 kb on - strand at 3568.848 kb on + strand at 3568.866 kb on - strand at 3568.866 kb on - strand at 3568.908 kb on - strand at 3568.909 kb on - strand at 3568.943 kb on + strand at 3568.954 kb on + strand at 3569.013 kb on - strand at 3569.074 kb on + strand at 3569.090 kb on + strand at 3569.090 kb on + strand at 3569.091 kb on - strand at 3569.125 kb on - strand at 3569.125 kb on - strand at 3569.304 kb on + strand at 3569.307 kb on + strand at 3569.307 kb on + strand at 3569.308 kb on - strand at 3569.336 kb on + strand, within PS417_16290 at 3569.387 kb on + strand, within PS417_16290 at 3569.404 kb on - strand, within PS417_16290 at 3569.404 kb on - strand, within PS417_16290 at 3569.519 kb on + strand at 3569.519 kb on + strand, within PS417_16290 at 3569.520 kb on - strand, within PS417_16290 at 3569.520 kb on - strand, within PS417_16290 at 3569.535 kb on - strand, within PS417_16290 at 3569.535 kb on - strand, within PS417_16290 at 3569.535 kb on - strand, within PS417_16290 at 3569.598 kb on + strand, within PS417_16290 at 3569.598 kb on + strand, within PS417_16290 at 3569.659 kb on + strand at 3569.668 kb on - strand at 3569.674 kb on - strand at 3569.724 kb on + strand at 3569.774 kb on + strand at 3569.853 kb on + strand at 3569.856 kb on + strand at 3569.947 kb on + strand, within PS417_16295 at 3569.947 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.989 kb on - strand, within PS417_16295 at 3570.040 kb on - strand at 3570.040 kb on - strand, within PS417_16295 at 3570.063 kb on - strand, within PS417_16295 at 3570.181 kb on - strand at 3570.209 kb on - strand at 3570.312 kb on - strand at 3570.318 kb on - strand at 3570.365 kb on - strand at 3570.365 kb on - strand at 3570.383 kb on - strand at 3570.425 kb on - strand at 3570.484 kb on + strand at 3570.535 kb on + strand at 3570.535 kb on + strand at 3570.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant remove 3,568,325 + PS417_16285 0.81 -2.0 3,568,325 + PS417_16285 0.81 +0.5 3,568,326 - PS417_16285 0.81 -1.9 3,568,338 - PS417_16285 0.82 -0.1 3,568,431 + -0.9 3,568,463 - +1.0 3,568,463 - +0.2 3,568,489 + +0.4 3,568,489 + -1.6 3,568,489 + +2.0 3,568,518 - -1.3 3,568,636 + +0.7 3,568,637 - +1.8 3,568,662 + -0.9 3,568,663 - -1.6 3,568,776 - +3.1 3,568,848 + +0.3 3,568,866 - -0.9 3,568,866 - +0.1 3,568,908 - +0.6 3,568,909 - +1.2 3,568,943 + -0.5 3,568,954 + -0.0 3,569,013 - -0.1 3,569,074 + -0.4 3,569,090 + +0.2 3,569,090 + -0.4 3,569,091 - +0.2 3,569,125 - -0.2 3,569,125 - -0.8 3,569,304 + -0.1 3,569,307 + +0.0 3,569,307 + -0.7 3,569,308 - -0.3 3,569,336 + PS417_16290 0.11 -0.9 3,569,387 + PS417_16290 0.25 -1.0 3,569,404 - PS417_16290 0.29 -0.6 3,569,404 - PS417_16290 0.29 +1.6 3,569,519 + -0.1 3,569,519 + PS417_16290 0.59 +0.0 3,569,520 - PS417_16290 0.59 -0.7 3,569,520 - PS417_16290 0.59 +0.4 3,569,535 - PS417_16290 0.63 -0.4 3,569,535 - PS417_16290 0.63 +0.2 3,569,535 - PS417_16290 0.63 -0.2 3,569,598 + PS417_16290 0.80 +1.9 3,569,598 + PS417_16290 0.80 -0.5 3,569,659 + +1.2 3,569,668 - -1.2 3,569,674 - +0.9 3,569,724 + -0.9 3,569,774 + +0.8 3,569,853 + -0.4 3,569,856 + -0.3 3,569,947 + PS417_16295 0.28 +0.4 3,569,947 + PS417_16295 0.28 -0.4 3,569,949 + PS417_16295 0.28 -0.4 3,569,949 + PS417_16295 0.28 -1.0 3,569,949 + PS417_16295 0.28 -0.2 3,569,949 + PS417_16295 0.28 -2.0 3,569,949 + PS417_16295 0.28 +0.0 3,569,949 + PS417_16295 0.28 -1.0 3,569,989 - PS417_16295 0.40 +1.2 3,570,040 - +1.4 3,570,040 - PS417_16295 0.56 +0.1 3,570,063 - PS417_16295 0.62 -0.8 3,570,181 - +0.3 3,570,209 - -1.9 3,570,312 - -0.1 3,570,318 - +0.0 3,570,365 - +0.1 3,570,365 - -0.1 3,570,383 - -0.9 3,570,425 - -0.8 3,570,484 + +0.5 3,570,535 + -1.2 3,570,535 + -0.2 3,570,536 - +0.9
Or see this region's nucleotide sequence