Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18980 and PS417_18990 are separated by 301 nucleotides PS417_18990 and PS417_18995 are separated by 57 nucleotides PS417_18995 and PS417_19000 are separated by 94 nucleotides
PS417_18980: PS417_18980 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 4,109,010 to 4,109,825
_18980
PS417_18990: PS417_18990 - membrane protein, at 4,110,127 to 4,111,338
_18990
PS417_18995: PS417_18995 - endonuclease, at 4,111,396 to 4,112,190
_18995
PS417_19000: PS417_19000 - MFS transporter, at 4,112,285 to 4,113,502
_19000
Position (kb)
4110
4111
4112 Strain fitness (log2 ratio)
-2
-1
0
1 at 4109.546 kb on - strand, within PS417_18980 at 4109.546 kb on - strand, within PS417_18980 at 4109.738 kb on - strand, within PS417_18980 at 4109.945 kb on - strand at 4110.051 kb on + strand at 4110.052 kb on - strand at 4110.052 kb on - strand at 4110.052 kb on - strand at 4110.441 kb on + strand, within PS417_18990 at 4110.441 kb on + strand, within PS417_18990 at 4110.441 kb on + strand, within PS417_18990 at 4110.442 kb on - strand, within PS417_18990 at 4110.564 kb on + strand, within PS417_18990 at 4110.602 kb on + strand, within PS417_18990 at 4111.385 kb on - strand at 4111.386 kb on + strand at 4111.386 kb on + strand at 4111.387 kb on - strand at 4111.473 kb on - strand at 4111.517 kb on + strand, within PS417_18995 at 4111.517 kb on + strand, within PS417_18995 at 4111.517 kb on + strand, within PS417_18995 at 4111.517 kb on + strand, within PS417_18995 at 4111.518 kb on - strand, within PS417_18995 at 4111.547 kb on + strand, within PS417_18995 at 4111.637 kb on + strand, within PS417_18995 at 4111.638 kb on - strand, within PS417_18995 at 4111.974 kb on - strand, within PS417_18995 at 4111.974 kb on - strand, within PS417_18995 at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.186 kb on + strand at 4112.187 kb on - strand at 4112.187 kb on - strand at 4112.187 kb on - strand at 4112.195 kb on + strand at 4112.195 kb on + strand at 4112.195 kb on + strand at 4112.196 kb on - strand at 4112.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 4,109,546 - PS417_18980 0.66 +1.0 4,109,546 - PS417_18980 0.66 -0.4 4,109,738 - PS417_18980 0.89 -0.2 4,109,945 - -0.2 4,110,051 + -1.3 4,110,052 - -0.3 4,110,052 - -0.2 4,110,052 - -0.7 4,110,441 + PS417_18990 0.26 -0.7 4,110,441 + PS417_18990 0.26 -1.2 4,110,441 + PS417_18990 0.26 -1.1 4,110,442 - PS417_18990 0.26 -0.4 4,110,564 + PS417_18990 0.36 -0.3 4,110,602 + PS417_18990 0.39 -1.1 4,111,385 - -0.7 4,111,386 + -0.5 4,111,386 + -0.7 4,111,387 - -0.3 4,111,473 - -0.2 4,111,517 + PS417_18995 0.15 +0.3 4,111,517 + PS417_18995 0.15 -0.3 4,111,517 + PS417_18995 0.15 -2.0 4,111,517 + PS417_18995 0.15 -0.8 4,111,518 - PS417_18995 0.15 -0.4 4,111,547 + PS417_18995 0.19 -1.1 4,111,637 + PS417_18995 0.30 -0.3 4,111,638 - PS417_18995 0.30 -0.5 4,111,974 - PS417_18995 0.73 -0.4 4,111,974 - PS417_18995 0.73 +0.9 4,112,186 + -0.1 4,112,186 + -0.3 4,112,186 + -0.6 4,112,186 + -0.1 4,112,186 + -0.0 4,112,187 - -0.5 4,112,187 - -0.3 4,112,187 - -0.7 4,112,195 + -1.2 4,112,195 + -0.5 4,112,195 + +0.1 4,112,196 - -0.6 4,112,265 - +0.1
Or see this region's nucleotide sequence