Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16765 and PS417_16770 are separated by 231 nucleotides PS417_16770 and PS417_16775 are separated by 12 nucleotides PS417_16775 and PS417_16780 are separated by 3 nucleotides
PS417_16765: PS417_16765 - transporter, at 3,656,981 to 3,658,228
_16765
PS417_16770: PS417_16770 - pyridine nucleotide-disulfide oxidoreductase, at 3,658,460 to 3,659,851
_16770
PS417_16775: PS417_16775 - branched-chain amino acid ABC transporter ATP-binding protein, at 3,659,864 to 3,660,631
_16775
PS417_16780: PS417_16780 - ABC transporter permease, at 3,660,635 to 3,661,975
_16780
Position (kb)
3658
3659
3660 Strain fitness (log2 ratio)
-1
0
1 at 3657.569 kb on + strand, within PS417_16765 at 3657.695 kb on - strand, within PS417_16765 at 3657.722 kb on - strand, within PS417_16765 at 3658.046 kb on + strand, within PS417_16765 at 3658.047 kb on - strand, within PS417_16765 at 3658.047 kb on - strand, within PS417_16765 at 3658.047 kb on - strand, within PS417_16765 at 3658.226 kb on + strand at 3658.226 kb on + strand at 3658.227 kb on - strand at 3658.227 kb on - strand at 3658.346 kb on + strand at 3658.347 kb on - strand at 3658.347 kb on - strand at 3658.347 kb on - strand at 3658.418 kb on + strand at 3658.418 kb on + strand at 3658.419 kb on - strand at 3658.502 kb on + strand at 3658.503 kb on - strand at 3659.221 kb on + strand, within PS417_16770 at 3659.517 kb on + strand, within PS417_16770 at 3659.604 kb on + strand, within PS417_16770 at 3659.703 kb on + strand, within PS417_16770 at 3659.703 kb on + strand, within PS417_16770 at 3659.703 kb on + strand, within PS417_16770 at 3659.823 kb on - strand at 3659.865 kb on + strand at 3659.881 kb on - strand at 3659.954 kb on + strand, within PS417_16775 at 3659.955 kb on - strand, within PS417_16775 at 3659.973 kb on + strand, within PS417_16775 at 3659.974 kb on - strand at 3659.974 kb on - strand, within PS417_16775 at 3660.073 kb on + strand, within PS417_16775 at 3660.518 kb on - strand, within PS417_16775 at 3660.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 3,657,569 + PS417_16765 0.47 -1.1 3,657,695 - PS417_16765 0.57 -0.1 3,657,722 - PS417_16765 0.59 +0.1 3,658,046 + PS417_16765 0.85 -0.0 3,658,047 - PS417_16765 0.85 -0.3 3,658,047 - PS417_16765 0.85 -0.4 3,658,047 - PS417_16765 0.85 +0.6 3,658,226 + -0.6 3,658,226 + +0.0 3,658,227 - -0.1 3,658,227 - +0.1 3,658,346 + -0.6 3,658,347 - +0.2 3,658,347 - +0.3 3,658,347 - -1.0 3,658,418 + +0.5 3,658,418 + +0.6 3,658,419 - -0.5 3,658,502 + -0.0 3,658,503 - +0.2 3,659,221 + PS417_16770 0.55 -0.6 3,659,517 + PS417_16770 0.76 -0.4 3,659,604 + PS417_16770 0.82 -0.4 3,659,703 + PS417_16770 0.89 -0.4 3,659,703 + PS417_16770 0.89 -0.3 3,659,703 + PS417_16770 0.89 -0.2 3,659,823 - +1.6 3,659,865 + -0.0 3,659,881 - +0.6 3,659,954 + PS417_16775 0.12 -0.3 3,659,955 - PS417_16775 0.12 +0.9 3,659,973 + PS417_16775 0.14 +0.7 3,659,974 - +0.1 3,659,974 - PS417_16775 0.14 +0.6 3,660,073 + PS417_16775 0.27 -0.4 3,660,518 - PS417_16775 0.85 +0.6 3,660,656 - -0.2
Or see this region's nucleotide sequence