Strain Fitness in Escherichia coli BW25113 around b3460

Experiment: M9 Acetate 0.5% aerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlivK and yhhK are separated by 423 nucleotidesyhhK and livJ are separated by 187 nucleotideslivJ and rpoH are separated by 270 nucleotides b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583 livK b3459: yhhK - hypothetical protein (NCBI), at 3,596,007 to 3,596,390 yhhK b3460: livJ - leucine/isoleucine/valine transporter subunit (NCBI), at 3,596,578 to 3,597,681 livJ b3461: rpoH - RNA polymerase sigma factor (NCBI), at 3,597,952 to 3,598,806 rpoH Position (kb) 3596 3597 3598Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7 8 9at 3595.637 kb on + strandat 3595.669 kb on + strandat 3595.968 kb on + strandat 3595.975 kb on + strandat 3596.005 kb on + strandat 3596.005 kb on - strandat 3596.005 kb on - strandat 3596.007 kb on + strandat 3596.011 kb on - strandat 3596.012 kb on - strandat 3596.013 kb on - strandat 3596.043 kb on + strandat 3596.048 kb on + strand, within yhhKat 3596.048 kb on + strand, within yhhKat 3596.056 kb on - strand, within yhhKat 3596.056 kb on - strand, within yhhKat 3596.061 kb on - strand, within yhhKat 3596.117 kb on + strand, within yhhKat 3596.128 kb on - strand, within yhhKat 3596.163 kb on - strand, within yhhKat 3596.163 kb on - strand, within yhhKat 3596.169 kb on + strand, within yhhKat 3596.209 kb on + strand, within yhhKat 3596.209 kb on + strand, within yhhKat 3596.251 kb on - strand, within yhhKat 3596.251 kb on - strand, within yhhKat 3596.267 kb on + strand, within yhhKat 3596.267 kb on + strand, within yhhKat 3596.288 kb on + strand, within yhhKat 3596.334 kb on + strand, within yhhKat 3596.420 kb on + strandat 3596.427 kb on + strandat 3596.427 kb on + strandat 3596.500 kb on + strandat 3596.588 kb on - strandat 3596.657 kb on + strandat 3596.737 kb on + strand, within livJat 3596.906 kb on + strand, within livJat 3596.914 kb on - strand, within livJat 3596.914 kb on - strand, within livJat 3596.994 kb on - strand, within livJat 3596.994 kb on - strand, within livJat 3597.006 kb on + strand, within livJat 3597.133 kb on + strand, within livJat 3597.141 kb on - strand, within livJat 3597.141 kb on - strand, within livJat 3597.141 kb on - strand, within livJat 3597.141 kb on - strand, within livJat 3597.148 kb on + strand, within livJat 3597.154 kb on + strand, within livJat 3597.154 kb on + strand, within livJat 3597.156 kb on - strand, within livJat 3597.161 kb on + strand, within livJat 3597.188 kb on + strand, within livJat 3597.188 kb on + strand, within livJat 3597.240 kb on + strand, within livJat 3597.240 kb on + strand, within livJat 3597.240 kb on - strand, within livJat 3597.250 kb on + strand, within livJat 3597.250 kb on + strand, within livJat 3597.251 kb on - strand, within livJat 3597.251 kb on - strand, within livJat 3597.251 kb on - strand, within livJat 3597.276 kb on + strand, within livJat 3597.312 kb on + strand, within livJat 3597.312 kb on + strand, within livJat 3597.320 kb on - strand, within livJat 3597.377 kb on + strand, within livJat 3597.377 kb on + strand, within livJat 3597.385 kb on - strand, within livJat 3597.385 kb on - strand, within livJat 3597.385 kb on - strand, within livJat 3597.385 kb on - strand, within livJat 3597.417 kb on - strand, within livJat 3597.519 kb on - strand, within livJat 3597.519 kb on - strand, within livJat 3597.556 kb on + strand, within livJat 3597.923 kb on + strandat 3597.931 kb on - strandat 3597.931 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Acetate 0.5% aerobic
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3,595,637 + -0.5
3,595,669 + -0.7
3,595,968 + -0.0
3,595,975 + -0.6
3,596,005 + +9.6
3,596,005 - -0.2
3,596,005 - -0.4
3,596,007 + -0.5
3,596,011 - -0.1
3,596,012 - +0.7
3,596,013 - +0.2
3,596,043 + -0.3
3,596,048 + yhhK b3459 0.11 +0.2
3,596,048 + yhhK b3459 0.11 -0.2
3,596,056 - yhhK b3459 0.13 +0.6
3,596,056 - yhhK b3459 0.13 +0.4
3,596,061 - yhhK b3459 0.14 +0.3
3,596,117 + yhhK b3459 0.29 +0.7
3,596,128 - yhhK b3459 0.32 +0.4
3,596,163 - yhhK b3459 0.41 +0.8
3,596,163 - yhhK b3459 0.41 +0.8
3,596,169 + yhhK b3459 0.42 +0.4
3,596,209 + yhhK b3459 0.53 +0.5
3,596,209 + yhhK b3459 0.53 -0.1
3,596,251 - yhhK b3459 0.64 +0.9
3,596,251 - yhhK b3459 0.64 +0.6
3,596,267 + yhhK b3459 0.68 +0.1
3,596,267 + yhhK b3459 0.68 +0.1
3,596,288 + yhhK b3459 0.73 -0.2
3,596,334 + yhhK b3459 0.85 -0.2
3,596,420 + -0.1
3,596,427 + -0.4
3,596,427 + -0.4
3,596,500 + -0.1
3,596,588 - -0.6
3,596,657 + -0.9
3,596,737 + livJ b3460 0.14 -0.4
3,596,906 + livJ b3460 0.30 -0.5
3,596,914 - livJ b3460 0.30 -1.1
3,596,914 - livJ b3460 0.30 -0.9
3,596,994 - livJ b3460 0.38 -1.3
3,596,994 - livJ b3460 0.38 -1.6
3,597,006 + livJ b3460 0.39 -0.5
3,597,133 + livJ b3460 0.50 -0.7
3,597,141 - livJ b3460 0.51 +0.0
3,597,141 - livJ b3460 0.51 -0.6
3,597,141 - livJ b3460 0.51 -0.6
3,597,141 - livJ b3460 0.51 -0.2
3,597,148 + livJ b3460 0.52 -0.7
3,597,154 + livJ b3460 0.52 -0.9
3,597,154 + livJ b3460 0.52 -0.4
3,597,156 - livJ b3460 0.52 -0.8
3,597,161 + livJ b3460 0.53 -0.5
3,597,188 + livJ b3460 0.55 -0.6
3,597,188 + livJ b3460 0.55 -1.2
3,597,240 + livJ b3460 0.60 -0.7
3,597,240 + livJ b3460 0.60 -0.3
3,597,240 - livJ b3460 0.60 -0.8
3,597,250 + livJ b3460 0.61 -1.0
3,597,250 + livJ b3460 0.61 -1.1
3,597,251 - livJ b3460 0.61 -0.6
3,597,251 - livJ b3460 0.61 -0.8
3,597,251 - livJ b3460 0.61 -0.2
3,597,276 + livJ b3460 0.63 -1.1
3,597,312 + livJ b3460 0.66 -0.5
3,597,312 + livJ b3460 0.66 -1.8
3,597,320 - livJ b3460 0.67 -0.8
3,597,377 + livJ b3460 0.72 -0.4
3,597,377 + livJ b3460 0.72 -0.8
3,597,385 - livJ b3460 0.73 -1.3
3,597,385 - livJ b3460 0.73 -0.4
3,597,385 - livJ b3460 0.73 -1.2
3,597,385 - livJ b3460 0.73 -0.6
3,597,417 - livJ b3460 0.76 -0.8
3,597,519 - livJ b3460 0.85 -0.8
3,597,519 - livJ b3460 0.85 -0.3
3,597,556 + livJ b3460 0.89 -1.0
3,597,923 + -0.2
3,597,931 - +0.2
3,597,931 - -0.5

Or see this region's nucleotide sequence