Strain Fitness in Escherichia coli BW25113 around b4491

Experiment: M9 Acetate 0.5% aerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1168 and ymgF are separated by 131 nucleotidesymgF and ycgH are separated by 399 nucleotidesycgH and b1171 are separated by 56 nucleotidesb1171 and ymgG are separated by 9 nucleotidesymgG and ymgI are separated by 1 nucleotides b1168: b1168 - putative proteases (VIMSS), at 1,216,551 to 1,218,074 b1168 b4520: ymgF - hypothetical protein (NCBI), at 1,218,206 to 1,218,424 ymgF b4491: ycgH - no description, at 1,218,824 to 1,221,471 ycgH b1171: b1171 - orf, hypothetical protein (VIMSS), at 1,221,528 to 1,221,857 b1171 b1172: ymgG - conserved protein, UPF0757 family (RefSeq), at 1,221,867 to 1,222,211 ymgG b4593: ymgI - hypothetical protein (RefSeq), at 1,222,213 to 1,222,386 ymgI Position (kb) 1218 1219 1220 1221 1222Strain fitness (log2 ratio) -3 -2 -1 0 1at 1217.856 kb on - strand, within b1168at 1217.861 kb on - strand, within b1168at 1217.891 kb on - strand, within b1168at 1217.891 kb on - strand, within b1168at 1217.891 kb on - strand, within b1168at 1218.016 kb on + strandat 1218.089 kb on + strandat 1218.096 kb on + strandat 1218.242 kb on - strand, within ymgFat 1218.284 kb on - strand, within ymgFat 1218.286 kb on - strand, within ymgFat 1218.301 kb on + strand, within ymgFat 1218.309 kb on - strand, within ymgFat 1218.311 kb on + strand, within ymgFat 1218.405 kb on + strandat 1218.414 kb on - strandat 1218.446 kb on + strandat 1218.450 kb on - strandat 1218.450 kb on - strandat 1218.454 kb on - strandat 1218.454 kb on - strandat 1218.468 kb on - strandat 1218.472 kb on - strandat 1218.478 kb on + strandat 1218.523 kb on + strandat 1219.855 kb on + strand, within ycgHat 1220.166 kb on - strand, within ycgHat 1220.183 kb on - strand, within ycgHat 1220.214 kb on - strand, within ycgHat 1220.226 kb on - strand, within ycgHat 1220.243 kb on - strand, within ycgHat 1220.243 kb on - strand, within ycgHat 1220.296 kb on + strand, within ycgHat 1220.304 kb on - strand, within ycgHat 1220.320 kb on + strand, within ycgHat 1220.339 kb on + strand, within ycgHat 1220.352 kb on + strand, within ycgHat 1220.489 kb on + strand, within ycgHat 1220.492 kb on - strand, within ycgHat 1220.533 kb on + strand, within ycgHat 1220.541 kb on - strand, within ycgHat 1220.723 kb on + strand, within ycgHat 1220.863 kb on + strand, within ycgHat 1220.907 kb on + strand, within ycgHat 1220.907 kb on + strand, within ycgHat 1220.922 kb on - strand, within ycgHat 1220.932 kb on - strand, within ycgHat 1220.932 kb on - strand, within ycgHat 1221.029 kb on - strand, within ycgHat 1221.103 kb on - strand, within ycgHat 1221.128 kb on + strand, within ycgHat 1221.137 kb on + strand, within ycgHat 1221.137 kb on + strand, within ycgHat 1221.271 kb on + strandat 1221.306 kb on + strandat 1221.396 kb on + strandat 1221.396 kb on + strandat 1221.424 kb on - strandat 1221.429 kb on + strandat 1221.429 kb on + strandat 1221.440 kb on + strandat 1221.440 kb on + strandat 1221.517 kb on - strandat 1221.549 kb on - strandat 1221.551 kb on + strandat 1221.552 kb on + strandat 1221.553 kb on + strandat 1221.553 kb on - strandat 1221.635 kb on + strand, within b1171at 1221.815 kb on - strand, within b1171at 1221.815 kb on - strand, within b1171at 1221.892 kb on - strandat 1221.934 kb on - strand, within ymgGat 1221.940 kb on + strand, within ymgGat 1222.035 kb on + strand, within ymgGat 1222.052 kb on + strand, within ymgGat 1222.060 kb on - strand, within ymgGat 1222.060 kb on - strand, within ymgGat 1222.135 kb on - strand, within ymgGat 1222.137 kb on + strand, within ymgGat 1222.141 kb on + strand, within ymgGat 1222.152 kb on + strand, within ymgGat 1222.160 kb on - strand, within ymgGat 1222.160 kb on - strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.162 kb on + strand, within ymgGat 1222.167 kb on + strand, within ymgGat 1222.170 kb on - strand, within ymgGat 1222.170 kb on - strand, within ymgGat 1222.180 kb on - strandat 1222.219 kb on + strandat 1222.243 kb on - strand, within ymgIat 1222.283 kb on - strand, within ymgIat 1222.330 kb on - strand, within ymgIat 1222.383 kb on - strandat 1222.415 kb on + strandat 1222.423 kb on + strandat 1222.423 kb on + strandat 1222.436 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Acetate 0.5% aerobic
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1,217,856 - b1168 b1168 0.86 -1.4
1,217,861 - b1168 b1168 0.86 -1.6
1,217,891 - b1168 b1168 0.88 -2.2
1,217,891 - b1168 b1168 0.88 -1.4
1,217,891 - b1168 b1168 0.88 -0.9
1,218,016 + +0.1
1,218,089 + -2.3
1,218,096 + -0.3
1,218,242 - ymgF b4520 0.16 -0.7
1,218,284 - ymgF b4520 0.36 -2.3
1,218,286 - ymgF b4520 0.37 -1.1
1,218,301 + ymgF b4520 0.43 -2.1
1,218,309 - ymgF b4520 0.47 -2.4
1,218,311 + ymgF b4520 0.48 -1.4
1,218,405 + -0.4
1,218,414 - -0.6
1,218,446 + -1.4
1,218,450 - -1.7
1,218,450 - +0.5
1,218,454 - -0.3
1,218,454 - -2.1
1,218,468 - -2.0
1,218,472 - -0.5
1,218,478 + -2.8
1,218,523 + -1.7
1,219,855 + ycgH b4491 0.39 -1.1
1,220,166 - ycgH b4491 0.51 -2.2
1,220,183 - ycgH b4491 0.51 -2.2
1,220,214 - ycgH b4491 0.52 -0.5
1,220,226 - ycgH b4491 0.53 -0.6
1,220,243 - ycgH b4491 0.54 -1.7
1,220,243 - ycgH b4491 0.54 -1.2
1,220,296 + ycgH b4491 0.56 -0.4
1,220,304 - ycgH b4491 0.56 -1.8
1,220,320 + ycgH b4491 0.56 +0.1
1,220,339 + ycgH b4491 0.57 -1.3
1,220,352 + ycgH b4491 0.58 -1.3
1,220,489 + ycgH b4491 0.63 -0.2
1,220,492 - ycgH b4491 0.63 -0.3
1,220,533 + ycgH b4491 0.65 +0.7
1,220,541 - ycgH b4491 0.65 +0.6
1,220,723 + ycgH b4491 0.72 +0.7
1,220,863 + ycgH b4491 0.77 +0.3
1,220,907 + ycgH b4491 0.79 +0.2
1,220,907 + ycgH b4491 0.79 +0.0
1,220,922 - ycgH b4491 0.79 +0.8
1,220,932 - ycgH b4491 0.80 -0.5
1,220,932 - ycgH b4491 0.80 -0.1
1,221,029 - ycgH b4491 0.83 -0.2
1,221,103 - ycgH b4491 0.86 -0.3
1,221,128 + ycgH b4491 0.87 -0.2
1,221,137 + ycgH b4491 0.87 -0.2
1,221,137 + ycgH b4491 0.87 +0.1
1,221,271 + +0.2
1,221,306 + +0.1
1,221,396 + -0.5
1,221,396 + +0.4
1,221,424 - -1.5
1,221,429 + +0.1
1,221,429 + -0.1
1,221,440 + +0.1
1,221,440 + +0.4
1,221,517 - -0.1
1,221,549 - -0.1
1,221,551 + -2.4
1,221,552 + -3.2
1,221,553 + -1.9
1,221,553 - +0.2
1,221,635 + b1171 b1171 0.32 -2.2
1,221,815 - b1171 b1171 0.87 +0.3
1,221,815 - b1171 b1171 0.87 +0.1
1,221,892 - +0.4
1,221,934 - ymgG b1172 0.19 +0.9
1,221,940 + ymgG b1172 0.21 -1.6
1,222,035 + ymgG b1172 0.49 -1.8
1,222,052 + ymgG b1172 0.54 +1.4
1,222,060 - ymgG b1172 0.56 +0.5
1,222,060 - ymgG b1172 0.56 -0.1
1,222,135 - ymgG b1172 0.78 +0.3
1,222,137 + ymgG b1172 0.78 -0.4
1,222,141 + ymgG b1172 0.79 +0.1
1,222,152 + ymgG b1172 0.83 -0.3
1,222,160 - ymgG b1172 0.85 -0.4
1,222,160 - ymgG b1172 0.85 +0.0
1,222,162 + ymgG b1172 0.86 -0.1
1,222,162 + ymgG b1172 0.86 -0.1
1,222,162 + ymgG b1172 0.86 -0.0
1,222,162 + ymgG b1172 0.86 -0.6
1,222,162 + ymgG b1172 0.86 -0.2
1,222,167 + ymgG b1172 0.87 -0.2
1,222,170 - ymgG b1172 0.88 -0.8
1,222,170 - ymgG b1172 0.88 +0.1
1,222,180 - -1.8
1,222,219 + -1.2
1,222,243 - ymgI b4593 0.17 -1.2
1,222,283 - ymgI b4593 0.40 -1.1
1,222,330 - ymgI b4593 0.67 -1.2
1,222,383 - -2.2
1,222,415 + -1.8
1,222,423 + -0.6
1,222,423 + -0.9
1,222,436 + -0.4

Or see this region's nucleotide sequence