Experiment: M9 Acetate 0.5% aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhsD and ybbC are separated by 39 nucleotides ybbC and ylbH are separated by 2 nucleotides ylbH and ybbD overlap by 20 nucleotides ybbD and ylbI are separated by 369 nucleotides ylbI and ylbG are separated by 2 nucleotides
b0497: rhsD - rhsD element protein (NCBI), at 522,485 to 526,765
rhsD
b0498: ybbC - hypothetical protein (NCBI), at 526,805 to 527,173
ybbC
b0499: ylbH - conserved protein, rhs-like (RefSeq), at 527,176 to 527,883
ylbH
b0500: ybbD - hypothetical protein (RefSeq), at 527,864 to 528,354
ybbD
b4632: ylbI - no description, at 528,724 to 528,816
ylbI
b0502: ylbG - no description, at 528,819 to 529,240
ylbG
Position (kb) 527
528 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 526.179 kb on + strand, within rhsD at 526.235 kb on + strand, within rhsD at 526.247 kb on + strand, within rhsD at 526.270 kb on + strand, within rhsD at 526.270 kb on + strand, within rhsD at 526.281 kb on + strand, within rhsD at 526.281 kb on + strand, within rhsD at 526.282 kb on + strand, within rhsD at 526.282 kb on + strand, within rhsD at 526.291 kb on + strand, within rhsD at 526.291 kb on + strand, within rhsD at 526.302 kb on + strand, within rhsD at 526.302 kb on + strand, within rhsD at 526.303 kb on + strand, within rhsD at 526.303 kb on + strand, within rhsD at 526.315 kb on + strand, within rhsD at 526.315 kb on + strand, within rhsD at 526.326 kb on + strand, within rhsD at 526.350 kb on + strand at 526.350 kb on + strand at 526.351 kb on + strand at 526.353 kb on + strand at 526.395 kb on + strand at 526.397 kb on + strand at 526.400 kb on + strand at 526.414 kb on + strand at 526.419 kb on + strand at 526.420 kb on + strand at 526.432 kb on + strand at 526.437 kb on + strand at 526.437 kb on + strand at 526.524 kb on + strand at 526.526 kb on + strand at 526.526 kb on + strand at 526.544 kb on + strand at 526.546 kb on + strand at 526.569 kb on + strand at 526.569 kb on + strand at 526.616 kb on + strand at 526.661 kb on + strand at 526.661 kb on + strand at 526.674 kb on + strand at 526.746 kb on + strand at 526.746 kb on + strand at 526.871 kb on + strand, within ybbC at 527.187 kb on - strand at 527.214 kb on - strand at 527.219 kb on - strand at 527.222 kb on + strand at 527.227 kb on - strand at 527.241 kb on + strand at 527.256 kb on + strand, within ylbH at 527.256 kb on + strand, within ylbH at 527.263 kb on + strand, within ylbH at 527.268 kb on + strand, within ylbH at 527.276 kb on - strand, within ylbH at 527.291 kb on - strand, within ylbH at 527.298 kb on + strand, within ylbH at 527.309 kb on + strand, within ylbH at 527.309 kb on + strand, within ylbH at 527.322 kb on - strand, within ylbH at 527.342 kb on - strand, within ylbH at 527.342 kb on - strand, within ylbH at 527.345 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.361 kb on + strand, within ylbH at 527.363 kb on + strand, within ylbH at 527.365 kb on + strand, within ylbH at 527.365 kb on + strand, within ylbH at 527.371 kb on - strand, within ylbH at 527.371 kb on - strand, within ylbH at 527.373 kb on + strand, within ylbH at 527.373 kb on + strand, within ylbH at 527.373 kb on + strand, within ylbH at 527.378 kb on + strand, within ylbH at 527.378 kb on + strand, within ylbH at 527.383 kb on + strand, within ylbH at 527.383 kb on + strand, within ylbH at 527.384 kb on + strand, within ylbH at 527.391 kb on - strand, within ylbH at 527.396 kb on + strand, within ylbH at 527.400 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.406 kb on + strand, within ylbH at 527.412 kb on - strand, within ylbH at 527.413 kb on + strand, within ylbH at 527.418 kb on + strand, within ylbH at 527.430 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.435 kb on + strand, within ylbH at 527.443 kb on + strand, within ylbH at 527.444 kb on + strand, within ylbH at 527.446 kb on + strand, within ylbH at 527.459 kb on + strand, within ylbH at 527.465 kb on + strand, within ylbH at 527.465 kb on + strand, within ylbH at 527.513 kb on + strand, within ylbH at 527.517 kb on + strand, within ylbH at 527.723 kb on + strand, within ylbH at 528.145 kb on - strand, within ybbD at 528.146 kb on - strand, within ybbD at 528.146 kb on - strand, within ybbD at 528.328 kb on + strand at 528.377 kb on - strand at 528.384 kb on + strand at 528.446 kb on + strand at 528.454 kb on + strand at 528.502 kb on + strand at 528.502 kb on + strand at 528.512 kb on + strand at 528.750 kb on - strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.759 kb on + strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.767 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.793 kb on - strand, within ylbI at 528.799 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.834 kb on - strand at 528.834 kb on - strand at 528.880 kb on + strand, within ylbG
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Acetate 0.5% aerobic remove 526,179 + rhsD b0497 0.86 +0.2 526,235 + rhsD b0497 0.88 +0.0 526,247 + rhsD b0497 0.88 +0.5 526,270 + rhsD b0497 0.88 +0.1 526,270 + rhsD b0497 0.88 +0.1 526,281 + rhsD b0497 0.89 +0.1 526,281 + rhsD b0497 0.89 +0.2 526,282 + rhsD b0497 0.89 +0.1 526,282 + rhsD b0497 0.89 -0.1 526,291 + rhsD b0497 0.89 -0.3 526,291 + rhsD b0497 0.89 -0.4 526,302 + rhsD b0497 0.89 +0.4 526,302 + rhsD b0497 0.89 +0.3 526,303 + rhsD b0497 0.89 +0.2 526,303 + rhsD b0497 0.89 -0.0 526,315 + rhsD b0497 0.89 +0.5 526,315 + rhsD b0497 0.89 +0.3 526,326 + rhsD b0497 0.90 +0.2 526,350 + +0.6 526,350 + -0.1 526,351 + +0.1 526,353 + -0.8 526,395 + -0.5 526,397 + -0.8 526,400 + -0.0 526,414 + -0.5 526,419 + -0.1 526,420 + +0.1 526,432 + -1.4 526,437 + +0.4 526,437 + -0.7 526,524 + -1.1 526,526 + -4.4 526,526 + -3.0 526,544 + -2.0 526,546 + -1.3 526,569 + -1.1 526,569 + -1.6 526,616 + -1.6 526,661 + -2.0 526,661 + -1.8 526,674 + -0.9 526,746 + +0.9 526,746 + -0.1 526,871 + ybbC b0498 0.18 +0.5 527,187 - -1.9 527,214 - -2.5 527,219 - -2.7 527,222 + -0.5 527,227 - -0.7 527,241 + -0.9 527,256 + ylbH b0499 0.11 -2.2 527,256 + ylbH b0499 0.11 -1.4 527,263 + ylbH b0499 0.12 -1.5 527,268 + ylbH b0499 0.13 -1.1 527,276 - ylbH b0499 0.14 -2.4 527,291 - ylbH b0499 0.16 -2.4 527,298 + ylbH b0499 0.17 -3.5 527,309 + ylbH b0499 0.19 -1.1 527,309 + ylbH b0499 0.19 -1.9 527,322 - ylbH b0499 0.21 -2.0 527,342 - ylbH b0499 0.23 -1.8 527,342 - ylbH b0499 0.23 -2.3 527,345 + ylbH b0499 0.24 -0.2 527,361 + ylbH b0499 0.26 -5.0 527,361 + ylbH b0499 0.26 -1.9 527,361 + ylbH b0499 0.26 -1.4 527,361 + ylbH b0499 0.26 -2.2 527,363 + ylbH b0499 0.26 -2.4 527,365 + ylbH b0499 0.27 -3.1 527,365 + ylbH b0499 0.27 -1.7 527,371 - ylbH b0499 0.28 -2.8 527,371 - ylbH b0499 0.28 -4.3 527,373 + ylbH b0499 0.28 -0.6 527,373 + ylbH b0499 0.28 -1.2 527,373 + ylbH b0499 0.28 -1.7 527,378 + ylbH b0499 0.29 -2.1 527,378 + ylbH b0499 0.29 -2.1 527,383 + ylbH b0499 0.29 -0.7 527,383 + ylbH b0499 0.29 -0.9 527,384 + ylbH b0499 0.29 -1.1 527,391 - ylbH b0499 0.30 -1.4 527,396 + ylbH b0499 0.31 -1.0 527,400 + ylbH b0499 0.32 -0.9 527,406 + ylbH b0499 0.32 -1.1 527,406 + ylbH b0499 0.32 -2.2 527,406 + ylbH b0499 0.32 +0.7 527,406 + ylbH b0499 0.32 -0.8 527,406 + ylbH b0499 0.32 +0.0 527,412 - ylbH b0499 0.33 -2.4 527,413 + ylbH b0499 0.33 -1.5 527,418 + ylbH b0499 0.34 -2.5 527,430 + ylbH b0499 0.36 -0.2 527,435 + ylbH b0499 0.37 -3.5 527,435 + ylbH b0499 0.37 -1.2 527,435 + ylbH b0499 0.37 -1.1 527,435 + ylbH b0499 0.37 -1.1 527,443 + ylbH b0499 0.38 -0.0 527,444 + ylbH b0499 0.38 -0.1 527,446 + ylbH b0499 0.38 -2.0 527,459 + ylbH b0499 0.40 -3.8 527,465 + ylbH b0499 0.41 +0.0 527,465 + ylbH b0499 0.41 -0.5 527,513 + ylbH b0499 0.48 -1.9 527,517 + ylbH b0499 0.48 -1.9 527,723 + ylbH b0499 0.77 +0.4 528,145 - ybbD b0500 0.57 -1.0 528,146 - ybbD b0500 0.57 -0.9 528,146 - ybbD b0500 0.57 -1.1 528,328 + -1.4 528,377 - +1.5 528,384 + -1.9 528,446 + -1.0 528,454 + -1.0 528,502 + -0.7 528,502 + -1.5 528,512 + +0.1 528,750 - ylbI b4632 0.28 -0.3 528,757 + ylbI b4632 0.35 -1.1 528,757 + ylbI b4632 0.35 +1.2 528,759 + ylbI b4632 0.38 -1.2 528,765 - ylbI b4632 0.44 -0.3 528,765 - ylbI b4632 0.44 +0.6 528,765 - ylbI b4632 0.44 +0.2 528,765 - ylbI b4632 0.44 +0.3 528,765 - ylbI b4632 0.44 +0.4 528,767 - ylbI b4632 0.46 +0.0 528,770 - ylbI b4632 0.49 +0.1 528,770 - ylbI b4632 0.49 +0.2 528,770 - ylbI b4632 0.49 +0.3 528,777 + ylbI b4632 0.57 -0.1 528,777 + ylbI b4632 0.57 -0.3 528,786 - ylbI b4632 0.67 +0.5 528,786 - ylbI b4632 0.67 +0.4 528,786 - ylbI b4632 0.67 +0.1 528,786 - ylbI b4632 0.67 +0.0 528,793 - ylbI b4632 0.74 +0.4 528,799 - ylbI b4632 0.81 -0.0 528,803 - ylbI b4632 0.85 -0.0 528,803 - ylbI b4632 0.85 +0.2 528,834 - -0.3 528,834 - -0.2 528,880 + ylbG b0502 0.14 -0.0
Or see this region's nucleotide sequence