Strain Fitness in Escherichia coli BW25113 around b3750

Experiment: M9 Acetate 0.5% aerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrbsA and rbsC are separated by 4 nucleotidesrbsC and rbsB are separated by 24 nucleotides b3749: rbsA - fused D-ribose transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,931,801 to 3,933,306 rbsA b3750: rbsC - ribose ABC transporter permease protein (NCBI), at 3,933,311 to 3,934,276 rbsC b3751: rbsB - D-ribose transporter subunit (NCBI), at 3,934,301 to 3,935,191 rbsB Position (kb) 3933 3934 3935Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7 8 9at 3932.340 kb on + strand, within rbsAat 3932.372 kb on + strand, within rbsAat 3932.383 kb on + strand, within rbsAat 3932.383 kb on + strand, within rbsAat 3932.383 kb on + strand, within rbsAat 3932.391 kb on - strand, within rbsAat 3932.456 kb on + strand, within rbsAat 3932.466 kb on + strand, within rbsAat 3932.481 kb on - strand, within rbsAat 3932.490 kb on - strand, within rbsAat 3932.542 kb on + strand, within rbsAat 3932.542 kb on + strand, within rbsAat 3932.555 kb on - strand, within rbsAat 3932.555 kb on - strand, within rbsAat 3932.578 kb on + strand, within rbsAat 3932.578 kb on + strand, within rbsAat 3932.589 kb on + strand, within rbsAat 3932.599 kb on + strand, within rbsAat 3932.599 kb on + strand, within rbsAat 3932.599 kb on + strand, within rbsAat 3932.599 kb on + strand, within rbsAat 3932.604 kb on - strand, within rbsAat 3932.684 kb on + strand, within rbsAat 3932.689 kb on + strand, within rbsAat 3932.689 kb on + strand, within rbsAat 3932.706 kb on + strand, within rbsAat 3932.706 kb on + strand, within rbsAat 3932.718 kb on + strand, within rbsAat 3932.720 kb on + strand, within rbsAat 3932.728 kb on + strand, within rbsAat 3932.728 kb on + strand, within rbsAat 3932.729 kb on + strand, within rbsAat 3932.731 kb on - strand, within rbsAat 3932.738 kb on + strand, within rbsAat 3932.738 kb on + strand, within rbsAat 3932.754 kb on - strand, within rbsAat 3932.754 kb on - strand, within rbsAat 3932.796 kb on - strand, within rbsAat 3932.827 kb on - strand, within rbsAat 3932.874 kb on + strand, within rbsAat 3932.874 kb on + strand, within rbsAat 3932.876 kb on - strand, within rbsAat 3932.876 kb on - strand, within rbsAat 3932.902 kb on - strand, within rbsAat 3932.927 kb on - strand, within rbsAat 3932.927 kb on - strand, within rbsAat 3932.968 kb on + strand, within rbsAat 3933.015 kb on + strand, within rbsAat 3933.015 kb on + strand, within rbsAat 3933.027 kb on - strand, within rbsAat 3933.064 kb on - strand, within rbsAat 3933.070 kb on - strand, within rbsAat 3933.070 kb on - strand, within rbsAat 3933.070 kb on - strand, within rbsAat 3933.082 kb on - strand, within rbsAat 3933.118 kb on - strand, within rbsAat 3933.118 kb on - strand, within rbsAat 3933.145 kb on + strand, within rbsAat 3933.164 kb on + strandat 3933.185 kb on - strandat 3933.200 kb on - strandat 3933.247 kb on + strandat 3933.256 kb on - strandat 3933.256 kb on - strandat 3933.258 kb on + strandat 3933.258 kb on + strandat 3933.262 kb on + strandat 3933.269 kb on + strandat 3933.282 kb on + strandat 3933.317 kb on + strandat 3933.317 kb on + strandat 3933.324 kb on - strandat 3933.345 kb on - strandat 3933.345 kb on - strandat 3933.384 kb on + strandat 3933.460 kb on + strand, within rbsCat 3933.481 kb on - strand, within rbsCat 3933.495 kb on + strand, within rbsCat 3933.495 kb on + strand, within rbsCat 3933.540 kb on + strand, within rbsCat 3933.548 kb on - strand, within rbsCat 3933.548 kb on - strand, within rbsCat 3933.592 kb on + strand, within rbsCat 3933.592 kb on + strand, within rbsCat 3933.755 kb on + strand, within rbsCat 3933.755 kb on + strand, within rbsCat 3933.768 kb on + strand, within rbsCat 3933.815 kb on - strand, within rbsCat 3933.815 kb on - strand, within rbsCat 3933.848 kb on - strand, within rbsCat 3933.856 kb on + strand, within rbsCat 3933.866 kb on + strand, within rbsCat 3933.876 kb on - strand, within rbsCat 3933.878 kb on - strand, within rbsCat 3933.883 kb on - strand, within rbsCat 3933.883 kb on - strand, within rbsCat 3933.886 kb on - strand, within rbsCat 3933.890 kb on + strand, within rbsCat 3933.898 kb on + strand, within rbsCat 3933.898 kb on + strand, within rbsCat 3933.898 kb on - strand, within rbsCat 3933.924 kb on - strand, within rbsCat 3933.924 kb on - strand, within rbsCat 3933.937 kb on + strand, within rbsCat 3933.950 kb on + strand, within rbsCat 3933.950 kb on + strand, within rbsCat 3934.001 kb on - strand, within rbsCat 3934.001 kb on - strand, within rbsCat 3934.003 kb on - strand, within rbsCat 3934.051 kb on - strand, within rbsCat 3934.056 kb on - strand, within rbsCat 3934.075 kb on + strandat 3934.094 kb on + strand, within rbsCat 3934.097 kb on - strand, within rbsCat 3934.101 kb on - strand, within rbsCat 3934.110 kb on - strand, within rbsCat 3934.269 kb on - strandat 3934.323 kb on + strandat 3934.323 kb on + strandat 3934.337 kb on + strandat 3934.383 kb on + strandat 3934.397 kb on - strand, within rbsBat 3934.467 kb on + strand, within rbsBat 3934.478 kb on + strand, within rbsBat 3934.489 kb on - strand, within rbsBat 3934.490 kb on + strand, within rbsBat 3934.528 kb on + strand, within rbsBat 3934.594 kb on - strand, within rbsBat 3934.594 kb on - strand, within rbsBat 3934.643 kb on - strand, within rbsBat 3934.684 kb on + strand, within rbsBat 3934.684 kb on + strand, within rbsBat 3934.687 kb on - strand, within rbsBat 3934.698 kb on - strand, within rbsBat 3934.698 kb on - strand, within rbsBat 3934.714 kb on + strand, within rbsBat 3934.714 kb on + strand, within rbsBat 3934.722 kb on - strand, within rbsBat 3934.751 kb on - strand, within rbsBat 3934.767 kb on + strand, within rbsBat 3934.774 kb on + strand, within rbsBat 3934.781 kb on + strand, within rbsBat 3934.842 kb on - strand, within rbsBat 3934.873 kb on + strand, within rbsBat 3934.881 kb on - strand, within rbsBat 3934.881 kb on - strand, within rbsBat 3934.908 kb on - strand, within rbsBat 3934.927 kb on + strand, within rbsBat 3934.930 kb on - strand, within rbsBat 3934.930 kb on - strand, within rbsBat 3934.931 kb on + strand, within rbsBat 3934.940 kb on - strand, within rbsBat 3934.950 kb on - strand, within rbsBat 3935.013 kb on + strand, within rbsBat 3935.022 kb on + strand, within rbsBat 3935.144 kb on + strandat 3935.155 kb on + strandat 3935.163 kb on - strandat 3935.201 kb on + strandat 3935.212 kb on - strandat 3935.222 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 Acetate 0.5% aerobic
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3,932,340 + rbsA b3749 0.36 +0.5
3,932,372 + rbsA b3749 0.38 +0.0
3,932,383 + rbsA b3749 0.39 +0.3
3,932,383 + rbsA b3749 0.39 -0.1
3,932,383 + rbsA b3749 0.39 +0.3
3,932,391 - rbsA b3749 0.39 +1.9
3,932,456 + rbsA b3749 0.43 +0.1
3,932,466 + rbsA b3749 0.44 -0.8
3,932,481 - rbsA b3749 0.45 +1.1
3,932,490 - rbsA b3749 0.46 +0.7
3,932,542 + rbsA b3749 0.49 -0.3
3,932,542 + rbsA b3749 0.49 -0.5
3,932,555 - rbsA b3749 0.50 +1.0
3,932,555 - rbsA b3749 0.50 +2.1
3,932,578 + rbsA b3749 0.52 -2.5
3,932,578 + rbsA b3749 0.52 -0.6
3,932,589 + rbsA b3749 0.52 -0.3
3,932,599 + rbsA b3749 0.53 -0.8
3,932,599 + rbsA b3749 0.53 -1.0
3,932,599 + rbsA b3749 0.53 -0.5
3,932,599 + rbsA b3749 0.53 -0.5
3,932,604 - rbsA b3749 0.53 +1.6
3,932,684 + rbsA b3749 0.59 -0.7
3,932,689 + rbsA b3749 0.59 +0.1
3,932,689 + rbsA b3749 0.59 +0.4
3,932,706 + rbsA b3749 0.60 -0.5
3,932,706 + rbsA b3749 0.60 -0.7
3,932,718 + rbsA b3749 0.61 -0.7
3,932,720 + rbsA b3749 0.61 +0.1
3,932,728 + rbsA b3749 0.62 -0.8
3,932,728 + rbsA b3749 0.62 +0.1
3,932,729 + rbsA b3749 0.62 -0.9
3,932,731 - rbsA b3749 0.62 +2.1
3,932,738 + rbsA b3749 0.62 -0.1
3,932,738 + rbsA b3749 0.62 -0.5
3,932,754 - rbsA b3749 0.63 +1.7
3,932,754 - rbsA b3749 0.63 +1.8
3,932,796 - rbsA b3749 0.66 +0.7
3,932,827 - rbsA b3749 0.68 +0.9
3,932,874 + rbsA b3749 0.71 -0.8
3,932,874 + rbsA b3749 0.71 -0.3
3,932,876 - rbsA b3749 0.71 +0.6
3,932,876 - rbsA b3749 0.71 +2.4
3,932,902 - rbsA b3749 0.73 +1.5
3,932,927 - rbsA b3749 0.75 -0.0
3,932,927 - rbsA b3749 0.75 +1.4
3,932,968 + rbsA b3749 0.77 -0.2
3,933,015 + rbsA b3749 0.81 -0.6
3,933,015 + rbsA b3749 0.81 -0.6
3,933,027 - rbsA b3749 0.81 +2.0
3,933,064 - rbsA b3749 0.84 +0.8
3,933,070 - rbsA b3749 0.84 +2.8
3,933,070 - rbsA b3749 0.84 +0.7
3,933,070 - rbsA b3749 0.84 +1.3
3,933,082 - rbsA b3749 0.85 +0.8
3,933,118 - rbsA b3749 0.87 +1.0
3,933,118 - rbsA b3749 0.87 +0.6
3,933,145 + rbsA b3749 0.89 -0.5
3,933,164 + +0.3
3,933,185 - +2.4
3,933,200 - +1.3
3,933,247 + -0.6
3,933,256 - +1.4
3,933,256 - +1.7
3,933,258 + +0.3
3,933,258 + -0.0
3,933,262 + -0.4
3,933,269 + -0.0
3,933,282 + -1.3
3,933,317 + +0.9
3,933,317 + -0.2
3,933,324 - +0.7
3,933,345 - +1.8
3,933,345 - +1.6
3,933,384 + +0.5
3,933,460 + rbsC b3750 0.15 +9.7
3,933,481 - rbsC b3750 0.18 +2.3
3,933,495 + rbsC b3750 0.19 +0.1
3,933,495 + rbsC b3750 0.19 +0.4
3,933,540 + rbsC b3750 0.24 -0.3
3,933,548 - rbsC b3750 0.25 +0.8
3,933,548 - rbsC b3750 0.25 +0.9
3,933,592 + rbsC b3750 0.29 +0.2
3,933,592 + rbsC b3750 0.29 -0.3
3,933,755 + rbsC b3750 0.46 -0.1
3,933,755 + rbsC b3750 0.46 -0.2
3,933,768 + rbsC b3750 0.47 -0.5
3,933,815 - rbsC b3750 0.52 +2.6
3,933,815 - rbsC b3750 0.52 +1.7
3,933,848 - rbsC b3750 0.56 +2.0
3,933,856 + rbsC b3750 0.56 +0.2
3,933,866 + rbsC b3750 0.57 -0.5
3,933,876 - rbsC b3750 0.58 +1.6
3,933,878 - rbsC b3750 0.59 +1.1
3,933,883 - rbsC b3750 0.59 +1.1
3,933,883 - rbsC b3750 0.59 +1.3
3,933,886 - rbsC b3750 0.60 +1.3
3,933,890 + rbsC b3750 0.60 +0.4
3,933,898 + rbsC b3750 0.61 -1.0
3,933,898 + rbsC b3750 0.61 -1.1
3,933,898 - rbsC b3750 0.61 +1.9
3,933,924 - rbsC b3750 0.63 +0.5
3,933,924 - rbsC b3750 0.63 +0.5
3,933,937 + rbsC b3750 0.65 -2.0
3,933,950 + rbsC b3750 0.66 -0.1
3,933,950 + rbsC b3750 0.66 -0.2
3,934,001 - rbsC b3750 0.71 +2.1
3,934,001 - rbsC b3750 0.71 +2.2
3,934,003 - rbsC b3750 0.72 +2.1
3,934,051 - rbsC b3750 0.77 +2.3
3,934,056 - rbsC b3750 0.77 +1.1
3,934,075 + -0.3
3,934,094 + rbsC b3750 0.81 -0.3
3,934,097 - rbsC b3750 0.81 +1.5
3,934,101 - rbsC b3750 0.82 +2.2
3,934,110 - rbsC b3750 0.83 +1.7
3,934,269 - +0.9
3,934,323 + -0.5
3,934,323 + -0.6
3,934,337 + -0.5
3,934,383 + +0.0
3,934,397 - rbsB b3751 0.11 +1.4
3,934,467 + rbsB b3751 0.19 -0.5
3,934,478 + rbsB b3751 0.20 -0.7
3,934,489 - rbsB b3751 0.21 +1.6
3,934,490 + rbsB b3751 0.21 -0.3
3,934,528 + rbsB b3751 0.25 -0.8
3,934,594 - rbsB b3751 0.33 +0.6
3,934,594 - rbsB b3751 0.33 +1.0
3,934,643 - rbsB b3751 0.38 +2.1
3,934,684 + rbsB b3751 0.43 -0.4
3,934,684 + rbsB b3751 0.43 -0.2
3,934,687 - rbsB b3751 0.43 +1.0
3,934,698 - rbsB b3751 0.45 +2.1
3,934,698 - rbsB b3751 0.45 +1.1
3,934,714 + rbsB b3751 0.46 -1.0
3,934,714 + rbsB b3751 0.46 -1.2
3,934,722 - rbsB b3751 0.47 +0.7
3,934,751 - rbsB b3751 0.51 +2.5
3,934,767 + rbsB b3751 0.52 -0.3
3,934,774 + rbsB b3751 0.53 -0.5
3,934,781 + rbsB b3751 0.54 -0.4
3,934,842 - rbsB b3751 0.61 +1.4
3,934,873 + rbsB b3751 0.64 -0.2
3,934,881 - rbsB b3751 0.65 +1.3
3,934,881 - rbsB b3751 0.65 +0.9
3,934,908 - rbsB b3751 0.68 +2.7
3,934,927 + rbsB b3751 0.70 -0.6
3,934,930 - rbsB b3751 0.71 +1.1
3,934,930 - rbsB b3751 0.71 +1.6
3,934,931 + rbsB b3751 0.71 -0.5
3,934,940 - rbsB b3751 0.72 +2.3
3,934,950 - rbsB b3751 0.73 +1.0
3,935,013 + rbsB b3751 0.80 -0.6
3,935,022 + rbsB b3751 0.81 -0.1
3,935,144 + -1.4
3,935,155 + -0.8
3,935,163 - +4.5
3,935,201 + -0.1
3,935,212 - +3.4
3,935,222 + -0.1

Or see this region's nucleotide sequence