Experiment: Growth at pH9 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5020 and PP_5021 are separated by 257 nucleotides PP_5021 and PP_5022 are separated by 109 nucleotides PP_5022 and PP_5023 overlap by 8 nucleotides
PP_5020: PP_5020 - putative methyl-accepting chemotaxis protein, at 5,717,302 to 5,719,218
_5020
PP_5021: PP_5021 - Methyl-accepting chemotaxis transducer, at 5,719,476 to 5,721,419
_5021
PP_5022: PP_5022 - Glutamine transport ATP-binding protein GlnQ, at 5,721,529 to 5,722,263
_5022
PP_5023: PP_5023 - Amino acid ABC transporter, permease protein, at 5,722,256 to 5,723,218
_5023
Position (kb) 5719
5720
5721
5722 Strain fitness (log2 ratio) -1
0
1 at 5718.722 kb on + strand, within PP_5020 at 5718.723 kb on - strand, within PP_5020 at 5718.960 kb on - strand, within PP_5020 at 5719.227 kb on - strand at 5719.343 kb on + strand at 5719.343 kb on + strand at 5719.343 kb on + strand at 5719.343 kb on + strand at 5719.344 kb on - strand at 5719.344 kb on - strand at 5719.344 kb on - strand at 5719.344 kb on - strand at 5719.345 kb on + strand at 5719.346 kb on - strand at 5719.381 kb on - strand at 5719.381 kb on - strand at 5719.395 kb on + strand at 5719.396 kb on - strand at 5719.633 kb on + strand at 5719.633 kb on + strand at 5719.633 kb on + strand at 5719.634 kb on - strand at 5719.634 kb on - strand at 5719.634 kb on - strand at 5719.822 kb on + strand, within PP_5021 at 5719.822 kb on + strand, within PP_5021 at 5719.823 kb on - strand, within PP_5021 at 5719.877 kb on - strand, within PP_5021 at 5720.055 kb on + strand, within PP_5021 at 5720.096 kb on + strand, within PP_5021 at 5720.121 kb on - strand, within PP_5021 at 5720.121 kb on - strand, within PP_5021 at 5720.398 kb on + strand, within PP_5021 at 5720.809 kb on + strand, within PP_5021 at 5720.809 kb on + strand, within PP_5021 at 5720.810 kb on - strand, within PP_5021 at 5720.810 kb on - strand at 5721.461 kb on + strand at 5721.461 kb on + strand at 5721.461 kb on + strand at 5721.461 kb on + strand at 5721.462 kb on - strand at 5721.462 kb on - strand at 5721.462 kb on - strand at 5721.530 kb on + strand at 5721.876 kb on + strand, within PP_5022 at 5721.877 kb on - strand, within PP_5022 at 5722.326 kb on + strand at 5722.326 kb on + strand at 5722.327 kb on - strand at 5722.327 kb on - strand at 5722.327 kb on - strand at 5722.361 kb on - strand, within PP_5023 at 5722.379 kb on - strand, within PP_5023 at 5722.379 kb on - strand, within PP_5023
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) D-Glucose remove 5,718,722 + PP_5020 0.74 +0.8 5,718,723 - PP_5020 0.74 -0.8 5,718,960 - PP_5020 0.86 -0.5 5,719,227 - -0.5 5,719,343 + +0.6 5,719,343 + -0.3 5,719,343 + +0.2 5,719,343 + -0.4 5,719,344 - +0.9 5,719,344 - +0.1 5,719,344 - -0.2 5,719,344 - -0.5 5,719,345 + -1.5 5,719,346 - -1.6 5,719,381 - +0.1 5,719,381 - -0.1 5,719,395 + +0.4 5,719,396 - +0.2 5,719,633 + -0.2 5,719,633 + -0.9 5,719,633 + +1.2 5,719,634 - -0.5 5,719,634 - +0.4 5,719,634 - +0.2 5,719,822 + PP_5021 0.18 +1.3 5,719,822 + PP_5021 0.18 +0.6 5,719,823 - PP_5021 0.18 +1.0 5,719,877 - PP_5021 0.21 +1.5 5,720,055 + PP_5021 0.30 -0.2 5,720,096 + PP_5021 0.32 -0.5 5,720,121 - PP_5021 0.33 +0.9 5,720,121 - PP_5021 0.33 -0.9 5,720,398 + PP_5021 0.47 +1.1 5,720,809 + PP_5021 0.69 +0.2 5,720,809 + PP_5021 0.69 +0.1 5,720,810 - PP_5021 0.69 +0.2 5,720,810 - -0.3 5,721,461 + -1.3 5,721,461 + +0.2 5,721,461 + -0.4 5,721,461 + -0.4 5,721,462 - -0.4 5,721,462 - +0.6 5,721,462 - +0.4 5,721,530 + +0.8 5,721,876 + PP_5022 0.47 +0.1 5,721,877 - PP_5022 0.47 +0.2 5,722,326 + -1.0 5,722,326 + -0.1 5,722,327 - +0.3 5,722,327 - +0.5 5,722,327 - -0.7 5,722,361 - PP_5023 0.11 -0.6 5,722,379 - PP_5023 0.13 -0.4 5,722,379 - PP_5023 0.13 +0.5
Or see this region's nucleotide sequence