Experiment: Growth at pH9 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0675 and PP_0676 are separated by 59 nucleotides PP_0676 and PP_0677 are separated by 67 nucleotides PP_0677 and PP_0678 are separated by 101 nucleotides
PP_0675: PP_0675 - glutamate dehydrogenase, NADP-dependent, at 788,763 to 790,112
_0675
PP_0676: PP_0676 - putative Transcription elongation factor, at 790,172 to 790,669
_0676
PP_0677: PP_0677 - putative Lipoprotein, at 790,737 to 791,093
_0677
PP_0678: PP_0678 - conserved exported protein of unknown function, at 791,195 to 793,048
_0678
Position (kb)
790
791
792 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 789.777 kb on - strand, within PP_0675 at 789.785 kb on + strand, within PP_0675 at 789.786 kb on - strand, within PP_0675 at 789.786 kb on - strand, within PP_0675 at 789.818 kb on + strand, within PP_0675 at 789.836 kb on + strand, within PP_0675 at 789.837 kb on - strand, within PP_0675 at 790.012 kb on - strand at 790.041 kb on + strand at 790.042 kb on - strand at 790.042 kb on - strand at 790.203 kb on - strand at 790.203 kb on - strand at 790.391 kb on + strand, within PP_0676 at 790.399 kb on + strand, within PP_0676 at 790.452 kb on + strand, within PP_0676 at 790.584 kb on + strand, within PP_0676 at 790.584 kb on + strand, within PP_0676 at 790.585 kb on - strand, within PP_0676 at 790.585 kb on - strand, within PP_0676 at 790.585 kb on - strand, within PP_0676 at 790.585 kb on - strand, within PP_0676 at 790.585 kb on - strand, within PP_0676 at 790.671 kb on + strand at 790.671 kb on + strand at 790.671 kb on + strand at 790.672 kb on - strand at 790.672 kb on - strand at 790.672 kb on - strand at 790.672 kb on - strand at 790.672 kb on - strand at 790.672 kb on - strand at 790.750 kb on + strand at 790.751 kb on - strand at 790.823 kb on - strand, within PP_0677 at 790.892 kb on - strand, within PP_0677 at 790.910 kb on - strand, within PP_0677 at 790.917 kb on + strand, within PP_0677 at 791.093 kb on + strand at 791.093 kb on + strand at 791.128 kb on + strand at 791.148 kb on - strand at 791.199 kb on + strand at 791.199 kb on + strand at 791.200 kb on - strand at 791.205 kb on + strand at 791.206 kb on - strand at 791.206 kb on - strand at 791.206 kb on - strand at 791.321 kb on + strand at 791.321 kb on + strand at 791.322 kb on - strand at 791.322 kb on - strand at 791.352 kb on + strand at 791.352 kb on + strand at 791.352 kb on + strand at 791.353 kb on - strand at 791.353 kb on - strand at 791.691 kb on + strand, within PP_0678 at 791.691 kb on + strand, within PP_0678 at 791.745 kb on + strand, within PP_0678 at 791.745 kb on + strand, within PP_0678 at 791.745 kb on + strand, within PP_0678 at 791.770 kb on - strand, within PP_0678 at 791.770 kb on - strand, within PP_0678 at 791.770 kb on - strand, within PP_0678 at 791.787 kb on + strand, within PP_0678 at 791.788 kb on - strand, within PP_0678 at 791.796 kb on + strand, within PP_0678 at 792.072 kb on - strand, within PP_0678
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) D-Glucose remove 789,777 - PP_0675 0.75 -3.0 789,785 + PP_0675 0.76 -0.4 789,786 - PP_0675 0.76 +0.3 789,786 - PP_0675 0.76 -0.3 789,818 + PP_0675 0.78 -0.9 789,836 + PP_0675 0.79 -0.2 789,837 - PP_0675 0.80 +0.2 790,012 - -0.9 790,041 + -0.6 790,042 - +0.4 790,042 - -0.1 790,203 - -0.5 790,203 - +0.8 790,391 + PP_0676 0.44 +0.3 790,399 + PP_0676 0.46 +0.2 790,452 + PP_0676 0.56 -0.9 790,584 + PP_0676 0.83 -0.4 790,584 + PP_0676 0.83 +0.6 790,585 - PP_0676 0.83 +0.1 790,585 - PP_0676 0.83 +0.6 790,585 - PP_0676 0.83 +0.3 790,585 - PP_0676 0.83 +0.3 790,585 - PP_0676 0.83 -0.3 790,671 + +1.1 790,671 + -1.1 790,671 + +1.3 790,672 - -0.6 790,672 - -0.9 790,672 - -0.2 790,672 - -0.3 790,672 - -0.4 790,672 - -0.8 790,750 + +0.8 790,751 - -0.2 790,823 - PP_0677 0.24 -0.0 790,892 - PP_0677 0.43 +0.8 790,910 - PP_0677 0.48 +0.8 790,917 + PP_0677 0.50 +0.2 791,093 + +0.1 791,093 + +0.7 791,128 + -0.4 791,148 - +0.3 791,199 + -0.4 791,199 + +0.0 791,200 - +0.4 791,205 + -0.5 791,206 - +0.3 791,206 - +1.0 791,206 - -0.8 791,321 + +0.5 791,321 + -1.4 791,322 - -1.1 791,322 - +1.4 791,352 + +0.7 791,352 + +0.4 791,352 + +0.4 791,353 - +0.1 791,353 - +0.4 791,691 + PP_0678 0.27 +1.2 791,691 + PP_0678 0.27 -0.4 791,745 + PP_0678 0.30 -0.8 791,745 + PP_0678 0.30 +0.9 791,745 + PP_0678 0.30 -0.7 791,770 - PP_0678 0.31 +0.0 791,770 - PP_0678 0.31 +0.4 791,770 - PP_0678 0.31 +0.2 791,787 + PP_0678 0.32 -0.5 791,788 - PP_0678 0.32 -0.1 791,796 + PP_0678 0.32 +1.1 792,072 - PP_0678 0.47 +0.6
Or see this region's nucleotide sequence