Experiment: M9 Glucose 2% aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjG and b1772 are separated by 9 nucleotides b1772 and ydjI are separated by 4 nucleotides ydjI and ydjJ are separated by 20 nucleotides
b1771: ydjG - predicted oxidoreductase (NCBI), at 1,853,015 to 1,853,995
ydjG
b1772: b1772 - putative kinase (VIMSS), at 1,854,005 to 1,854,952
b1772
b1773: ydjI - predicted aldolase (NCBI), at 1,854,957 to 1,855,793
ydjI
b1774: ydjJ - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,855,814 to 1,856,857
ydjJ
Position (kb)
1854
1855 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1853.029 kb on - strand at 1853.029 kb on - strand at 1853.048 kb on - strand at 1853.102 kb on + strand at 1853.110 kb on - strand at 1853.170 kb on + strand, within ydjG at 1853.192 kb on - strand, within ydjG at 1853.368 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.448 kb on + strand, within ydjG at 1853.537 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.751 kb on + strand, within ydjG at 1853.763 kb on - strand, within ydjG at 1853.919 kb on - strand at 1853.937 kb on - strand at 1853.947 kb on - strand at 1854.143 kb on - strand, within b1772 at 1854.143 kb on - strand, within b1772 at 1854.178 kb on - strand, within b1772 at 1854.178 kb on - strand, within b1772 at 1854.223 kb on - strand, within b1772 at 1854.246 kb on - strand, within b1772 at 1854.321 kb on - strand, within b1772 at 1854.389 kb on + strand, within b1772 at 1854.397 kb on - strand, within b1772 at 1854.425 kb on - strand, within b1772 at 1854.425 kb on - strand, within b1772 at 1854.461 kb on - strand, within b1772 at 1854.461 kb on - strand, within b1772 at 1854.550 kb on - strand, within b1772 at 1854.626 kb on + strand, within b1772 at 1854.644 kb on - strand, within b1772 at 1854.644 kb on - strand, within b1772 at 1854.651 kb on + strand, within b1772 at 1854.652 kb on + strand, within b1772 at 1854.670 kb on + strand, within b1772 at 1854.670 kb on + strand, within b1772 at 1854.935 kb on - strand at 1854.935 kb on - strand at 1855.036 kb on - strand at 1855.081 kb on - strand, within ydjI at 1855.087 kb on - strand, within ydjI at 1855.097 kb on - strand, within ydjI at 1855.184 kb on - strand, within ydjI at 1855.333 kb on + strand, within ydjI at 1855.336 kb on + strand, within ydjI at 1855.336 kb on + strand, within ydjI at 1855.341 kb on + strand, within ydjI at 1855.378 kb on + strand, within ydjI at 1855.403 kb on + strand, within ydjI at 1855.411 kb on + strand, within ydjI at 1855.550 kb on + strand, within ydjI at 1855.591 kb on + strand, within ydjI at 1855.593 kb on + strand, within ydjI at 1855.593 kb on + strand, within ydjI at 1855.868 kb on - strand at 1855.880 kb on - strand at 1855.890 kb on - strand at 1855.890 kb on - strand at 1855.907 kb on - strand at 1855.944 kb on - strand, within ydjJ at 1855.944 kb on - strand, within ydjJ
Per-strain Table
Position Strand Gene LocusTag Fraction M9 Glucose 2% aerobic remove 1,853,029 - +0.2 1,853,029 - +0.2 1,853,048 - -0.2 1,853,102 + +0.1 1,853,110 - +0.1 1,853,170 + ydjG b1771 0.16 +0.4 1,853,192 - ydjG b1771 0.18 +0.2 1,853,368 - ydjG b1771 0.36 -0.0 1,853,425 - ydjG b1771 0.42 -0.0 1,853,425 - ydjG b1771 0.42 +0.5 1,853,448 + ydjG b1771 0.44 +0.1 1,853,537 - ydjG b1771 0.53 -0.0 1,853,621 - ydjG b1771 0.62 +0.6 1,853,621 - ydjG b1771 0.62 +0.3 1,853,666 - ydjG b1771 0.66 -0.1 1,853,666 - ydjG b1771 0.66 +0.3 1,853,751 + ydjG b1771 0.75 -1.4 1,853,763 - ydjG b1771 0.76 +0.4 1,853,919 - +0.4 1,853,937 - -0.1 1,853,947 - +0.7 1,854,143 - b1772 b1772 0.15 +0.4 1,854,143 - b1772 b1772 0.15 -0.1 1,854,178 - b1772 b1772 0.18 -0.0 1,854,178 - b1772 b1772 0.18 -0.1 1,854,223 - b1772 b1772 0.23 +0.3 1,854,246 - b1772 b1772 0.25 -1.2 1,854,321 - b1772 b1772 0.33 -0.5 1,854,389 + b1772 b1772 0.41 -1.6 1,854,397 - b1772 b1772 0.41 -2.6 1,854,425 - b1772 b1772 0.44 -0.3 1,854,425 - b1772 b1772 0.44 +0.1 1,854,461 - b1772 b1772 0.48 -1.6 1,854,461 - b1772 b1772 0.48 -1.3 1,854,550 - b1772 b1772 0.57 -1.7 1,854,626 + b1772 b1772 0.66 +1.1 1,854,644 - b1772 b1772 0.67 -0.3 1,854,644 - b1772 b1772 0.67 -0.9 1,854,651 + b1772 b1772 0.68 -3.0 1,854,652 + b1772 b1772 0.68 -3.1 1,854,670 + b1772 b1772 0.70 -1.5 1,854,670 + b1772 b1772 0.70 -1.5 1,854,935 - -2.2 1,854,935 - -2.7 1,855,036 - +0.2 1,855,081 - ydjI b1773 0.15 +0.1 1,855,087 - ydjI b1773 0.16 -1.0 1,855,097 - ydjI b1773 0.17 +0.3 1,855,184 - ydjI b1773 0.27 +0.1 1,855,333 + ydjI b1773 0.45 -1.3 1,855,336 + ydjI b1773 0.45 -2.1 1,855,336 + ydjI b1773 0.45 -2.2 1,855,341 + ydjI b1773 0.46 -0.5 1,855,378 + ydjI b1773 0.50 -1.8 1,855,403 + ydjI b1773 0.53 -3.3 1,855,411 + ydjI b1773 0.54 -1.2 1,855,550 + ydjI b1773 0.71 -4.0 1,855,591 + ydjI b1773 0.76 -1.7 1,855,593 + ydjI b1773 0.76 -2.1 1,855,593 + ydjI b1773 0.76 -3.0 1,855,868 - +0.2 1,855,880 - +0.2 1,855,890 - +0.3 1,855,890 - +0.4 1,855,907 - +0.3 1,855,944 - ydjJ b1774 0.12 +0.3 1,855,944 - ydjJ b1774 0.12 +0.5
Or see this region's nucleotide sequence