Strain Fitness in Escherichia coli BW25113 around b4488
Experiment: M9 Glucose 2% aerobic
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | M9 Glucose 2% aerobic |
---|---|---|---|---|---|
remove | |||||
3,947,746 | + | yifB | b3765 | 0.84 | +0.2 |
3,947,833 | - | yifB | b3765 | 0.89 | +0.1 |
3,947,836 | - | yifB | b3765 | 0.90 | -0.2 |
3,947,841 | + | -0.1 | |||
3,947,903 | + | -0.0 | |||
3,947,903 | + | +0.4 | |||
3,947,984 | - | -0.5 | |||
3,948,303 | + | -1.1 | |||
3,948,305 | + | +0.3 | |||
3,948,364 | + | ilvL | b3766 | 0.19 | +0.0 |
3,948,375 | + | ilvL | b3766 | 0.30 | +0.8 |
3,948,383 | - | ilvL | b3766 | 0.38 | -0.5 |
3,948,410 | + | ilvL | b3766 | 0.66 | +0.4 |
3,948,410 | + | ilvL | b3766 | 0.66 | +0.9 |
3,948,410 | + | ilvL | b3766 | 0.66 | +0.3 |
3,948,531 | - | -4.1 | |||
3,948,579 | + | +0.0 | |||
3,948,580 | + | +0.1 | |||
3,948,580 | + | -1.3 | |||
3,948,604 | + | -2.2 | |||
3,948,604 | + | -1.9 | |||
3,948,620 | + | -1.2 | |||
3,948,620 | + | -0.4 | |||
3,948,624 | + | -0.2 | |||
3,948,633 | - | -0.9 | |||
3,948,633 | - | -1.9 | |||
3,948,633 | - | -4.7 | |||
3,948,682 | + | -1.0 | |||
3,948,721 | + | +0.6 | |||
3,948,726 | + | +0.3 | |||
3,948,748 | + | ilvG | b4488 | 0.10 | -1.1 |
3,948,748 | + | ilvG | b4488 | 0.10 | -4.7 |
3,948,781 | + | ilvG | b4488 | 0.12 | -1.1 |
3,948,815 | + | ilvG | b4488 | 0.14 | -0.4 |
3,948,815 | + | ilvG | b4488 | 0.14 | -1.1 |
3,948,851 | + | ilvG | b4488 | 0.16 | -2.3 |
3,948,851 | + | ilvG | b4488 | 0.16 | -1.3 |
3,948,851 | + | ilvG | b4488 | 0.16 | -1.7 |
3,948,859 | - | ilvG | b4488 | 0.17 | -4.2 |
3,948,933 | + | ilvG | b4488 | 0.21 | +0.4 |
3,948,939 | + | ilvG | b4488 | 0.22 | -0.7 |
3,948,944 | + | ilvG | b4488 | 0.22 | +0.0 |
3,948,947 | - | ilvG | b4488 | 0.22 | -4.3 |
3,948,947 | - | ilvG | b4488 | 0.22 | -3.4 |
3,948,966 | - | ilvG | b4488 | 0.23 | -4.3 |
3,949,037 | - | ilvG | b4488 | 0.28 | -2.3 |
3,949,042 | - | ilvG | b4488 | 0.28 | -2.6 |
3,949,083 | + | ilvG | b4488 | 0.30 | -0.1 |
3,949,083 | + | ilvG | b4488 | 0.30 | +0.8 |
3,949,136 | + | ilvG | b4488 | 0.34 | -0.7 |
3,949,136 | + | ilvG | b4488 | 0.34 | +0.4 |
3,949,150 | + | ilvG | b4488 | 0.34 | +0.3 |
3,949,150 | + | ilvG | b4488 | 0.34 | +0.3 |
3,949,219 | + | ilvG | b4488 | 0.39 | +0.1 |
3,949,265 | + | ilvG | b4488 | 0.41 | +0.9 |
3,949,308 | - | ilvG | b4488 | 0.44 | -3.1 |
3,949,330 | - | ilvG | b4488 | 0.45 | -2.9 |
3,949,360 | + | ilvG | b4488 | 0.47 | +1.1 |
3,949,360 | + | ilvG | b4488 | 0.47 | +0.2 |
3,949,368 | - | ilvG | b4488 | 0.48 | -3.5 |
3,949,370 | - | ilvG | b4488 | 0.48 | -2.7 |
3,949,438 | - | ilvG | b4488 | 0.52 | -5.9 |
3,949,443 | + | ilvG | b4488 | 0.52 | +0.7 |
3,949,443 | + | ilvG | b4488 | 0.52 | +0.8 |
3,949,443 | + | ilvG | b4488 | 0.52 | +0.8 |
3,949,443 | + | ilvG | b4488 | 0.52 | +0.8 |
3,949,461 | - | ilvG | b4488 | 0.53 | -4.3 |
3,949,462 | + | ilvG | b4488 | 0.53 | +0.3 |
3,949,462 | + | ilvG | b4488 | 0.53 | +0.3 |
3,949,462 | + | ilvG | b4488 | 0.53 | +0.7 |
3,949,513 | - | ilvG | b4488 | 0.57 | -3.9 |
3,949,533 | + | ilvG | b4488 | 0.58 | -0.0 |
3,949,533 | + | ilvG | b4488 | 0.58 | +1.0 |
3,949,533 | + | ilvG | b4488 | 0.58 | +0.8 |
3,949,566 | + | ilvG | b4488 | 0.60 | +0.5 |
3,949,566 | + | ilvG | b4488 | 0.60 | +0.9 |
3,949,571 | + | ilvG | b4488 | 0.60 | +1.2 |
3,949,571 | + | ilvG | b4488 | 0.60 | +1.1 |
3,949,577 | - | ilvG | b4488 | 0.60 | -3.6 |
3,949,618 | - | ilvG | b4488 | 0.63 | -2.2 |
3,949,714 | + | ilvG | b4488 | 0.69 | +1.0 |
3,949,717 | + | ilvG | b4488 | 0.69 | +0.9 |
3,949,722 | + | ilvG | b4488 | 0.69 | +0.4 |
3,949,722 | + | ilvG | b4488 | 0.69 | +0.5 |
3,949,725 | - | ilvG | b4488 | 0.69 | -2.7 |
3,949,725 | - | ilvG | b4488 | 0.69 | -1.7 |
3,949,725 | - | ilvG | b4488 | 0.69 | -1.9 |
3,949,732 | + | ilvG | b4488 | 0.70 | +1.1 |
3,949,732 | + | ilvG | b4488 | 0.70 | +0.7 |
3,949,789 | + | ilvG | b4488 | 0.73 | +1.3 |
3,949,837 | - | ilvG | b4488 | 0.76 | -3.2 |
3,949,839 | - | ilvG | b4488 | 0.76 | -1.5 |
3,949,839 | - | ilvG | b4488 | 0.76 | -3.8 |
3,949,863 | - | ilvG | b4488 | 0.78 | -1.1 |
3,949,951 | + | ilvG | b4488 | 0.83 | +1.2 |
3,949,959 | - | ilvG | b4488 | 0.84 | -1.8 |
3,950,004 | + | ilvG | b4488 | 0.86 | +0.2 |
3,950,004 | + | ilvG | b4488 | 0.86 | +0.6 |
3,950,063 | + | +1.2 | |||
3,950,064 | + | +1.0 | |||
3,950,106 | + | +0.3 | |||
3,950,109 | - | -0.2 | |||
3,950,109 | - | +0.3 | |||
3,950,111 | + | +1.2 | |||
3,950,119 | - | +0.4 | |||
3,950,119 | - | +0.3 | |||
3,950,119 | - | +0.1 | |||
3,950,121 | + | +1.1 | |||
3,950,121 | + | +1.8 | |||
3,950,127 | + | +1.0 | |||
3,950,140 | - | +0.8 | |||
3,950,140 | - | +0.7 | |||
3,950,228 | + | +1.6 | |||
3,950,251 | - | ilvM | b3769 | 0.10 | +0.4 |
3,950,301 | - | ilvM | b3769 | 0.29 | +0.1 |
3,950,301 | - | ilvM | b3769 | 0.29 | -0.5 |
3,950,381 | + | ilvM | b3769 | 0.59 | +1.4 |
3,950,381 | + | ilvM | b3769 | 0.59 | +1.0 |
3,950,381 | + | ilvM | b3769 | 0.59 | +0.9 |
3,950,381 | + | ilvM | b3769 | 0.59 | +1.3 |
3,950,381 | + | ilvM | b3769 | 0.59 | +0.7 |
3,950,389 | - | ilvM | b3769 | 0.62 | -1.3 |
3,950,456 | + | ilvM | b3769 | 0.88 | -3.4 |
3,950,475 | - | -1.2 | |||
3,950,475 | - | -1.1 | |||
3,950,478 | - | -1.5 | |||
3,950,478 | - | -0.3 | |||
3,950,592 | - | -3.2 | |||
3,950,599 | - | -4.4 | |||
3,950,631 | + | ilvE | b3770 | 0.13 | -3.8 |
3,950,631 | + | ilvE | b3770 | 0.13 | -2.3 |
3,950,654 | + | ilvE | b3770 | 0.16 | -5.3 |
3,950,654 | + | ilvE | b3770 | 0.16 | -5.7 |
3,950,654 | + | ilvE | b3770 | 0.16 | -3.6 |
3,950,675 | + | ilvE | b3770 | 0.18 | -3.5 |
3,950,675 | + | ilvE | b3770 | 0.18 | -4.9 |
3,950,695 | - | ilvE | b3770 | 0.20 | -4.1 |
3,950,713 | + | ilvE | b3770 | 0.22 | -3.4 |
3,950,789 | + | ilvE | b3770 | 0.30 | -5.0 |
3,950,789 | + | ilvE | b3770 | 0.30 | -4.4 |
3,950,789 | + | ilvE | b3770 | 0.30 | -3.7 |
3,950,797 | - | ilvE | b3770 | 0.31 | -4.5 |
3,950,797 | - | ilvE | b3770 | 0.31 | -2.7 |
3,950,797 | - | ilvE | b3770 | 0.31 | -4.0 |
3,950,798 | - | ilvE | b3770 | 0.31 | -4.1 |
3,950,822 | - | ilvE | b3770 | 0.34 | -5.8 |
3,950,822 | - | ilvE | b3770 | 0.34 | -2.9 |
3,950,828 | - | ilvE | b3770 | 0.35 | -5.1 |
3,950,832 | + | ilvE | b3770 | 0.35 | -4.6 |
3,950,851 | + | ilvE | b3770 | 0.37 | -5.5 |
3,950,851 | + | ilvE | b3770 | 0.37 | -6.5 |
3,950,942 | + | ilvE | b3770 | 0.47 | -3.1 |
3,950,948 | - | ilvE | b3770 | 0.47 | -4.3 |
3,950,952 | + | ilvE | b3770 | 0.48 | -3.8 |
3,950,967 | - | ilvE | b3770 | 0.49 | -4.2 |
3,951,009 | - | ilvE | b3770 | 0.54 | -3.4 |
3,951,022 | - | ilvE | b3770 | 0.55 | -3.5 |
3,951,054 | + | ilvE | b3770 | 0.59 | -4.9 |
3,951,057 | - | ilvE | b3770 | 0.59 | -4.2 |
3,951,164 | + | ilvE | b3770 | 0.71 | -2.0 |
3,951,164 | + | ilvE | b3770 | 0.71 | -4.8 |
3,951,168 | - | ilvE | b3770 | 0.71 | -4.0 |
3,951,170 | + | ilvE | b3770 | 0.71 | -4.1 |
3,951,171 | - | ilvE | b3770 | 0.71 | -4.1 |
Or see this region's nucleotide sequence