Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5093 and PP_5094 are separated by 53 nucleotides PP_5094 and PP_5095 are separated by 38 nucleotides PP_5095 and PP_5096 are separated by 10 nucleotides PP_5096 and PP_5097 are separated by 99 nucleotides
PP_5093: PP_5093 - twitching motility protein, at 5,816,943 to 5,817,953
_5093
PP_5094: PP_5094 - putative enzyme with PLP binding domain, at 5,818,007 to 5,818,693
_5094
PP_5095: PP_5095 - pyrroline-5-carboxylate reductase, at 5,818,732 to 5,819,550
_5095
PP_5096: PP_5096 - putative factor involved in shape determination, at 5,819,561 to 5,820,151
_5096
PP_5097: PP_5097 - homoserine O-acetyltransferase, at 5,820,251 to 5,821,390
_5097
Position (kb) 5818
5819
5820 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5817.833 kb on + strand, within PP_5093 at 5817.941 kb on + strand at 5817.941 kb on + strand at 5817.941 kb on + strand at 5817.941 kb on + strand at 5817.942 kb on - strand at 5817.942 kb on - strand at 5817.942 kb on - strand at 5817.942 kb on - strand at 5817.942 kb on - strand at 5817.969 kb on + strand at 5817.969 kb on + strand at 5817.970 kb on - strand at 5817.970 kb on - strand at 5817.970 kb on - strand at 5818.017 kb on + strand at 5818.018 kb on - strand at 5818.018 kb on - strand at 5818.178 kb on + strand, within PP_5094 at 5818.182 kb on + strand, within PP_5094 at 5818.183 kb on - strand, within PP_5094 at 5818.183 kb on - strand, within PP_5094 at 5818.405 kb on + strand, within PP_5094 at 5818.405 kb on + strand, within PP_5094 at 5818.405 kb on + strand, within PP_5094 at 5818.406 kb on - strand, within PP_5094 at 5818.431 kb on + strand, within PP_5094 at 5818.746 kb on + strand at 5818.746 kb on + strand at 5818.746 kb on + strand at 5818.747 kb on - strand at 5818.747 kb on - strand at 5818.747 kb on - strand at 5818.747 kb on - strand at 5818.747 kb on - strand at 5818.747 kb on - strand at 5818.968 kb on - strand, within PP_5095 at 5819.128 kb on + strand, within PP_5095 at 5819.129 kb on - strand, within PP_5095 at 5819.190 kb on - strand, within PP_5095 at 5819.548 kb on + strand at 5819.548 kb on + strand at 5819.549 kb on - strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.558 kb on + strand at 5819.559 kb on - strand at 5819.559 kb on - strand at 5819.669 kb on + strand, within PP_5096 at 5819.669 kb on + strand, within PP_5096 at 5819.670 kb on - strand, within PP_5096 at 5819.670 kb on - strand, within PP_5096 at 5819.670 kb on - strand, within PP_5096 at 5819.740 kb on + strand, within PP_5096 at 5819.825 kb on + strand, within PP_5096 at 5819.825 kb on + strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.826 kb on - strand, within PP_5096 at 5819.830 kb on + strand, within PP_5096 at 5819.830 kb on + strand, within PP_5096 at 5819.831 kb on - strand, within PP_5096 at 5819.831 kb on - strand, within PP_5096 at 5820.216 kb on + strand at 5820.222 kb on + strand at 5820.222 kb on + strand at 5820.222 kb on + strand at 5820.222 kb on + strand at 5820.223 kb on - strand at 5820.289 kb on - strand at 5820.354 kb on - strand at 5820.366 kb on - strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.374 kb on + strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.375 kb on - strand, within PP_5097 at 5820.452 kb on + strand, within PP_5097
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 5,817,833 + PP_5093 0.88 -0.8 5,817,941 + +0.4 5,817,941 + -0.1 5,817,941 + +0.5 5,817,941 + -0.1 5,817,942 - +0.2 5,817,942 - -1.5 5,817,942 - +0.8 5,817,942 - -2.7 5,817,942 - +0.1 5,817,969 + +0.8 5,817,969 + -1.1 5,817,970 - -1.0 5,817,970 - -1.1 5,817,970 - -0.1 5,818,017 + -1.7 5,818,018 - -0.9 5,818,018 - -0.2 5,818,178 + PP_5094 0.25 -0.1 5,818,182 + PP_5094 0.25 -0.3 5,818,183 - PP_5094 0.26 -1.1 5,818,183 - PP_5094 0.26 -0.4 5,818,405 + PP_5094 0.58 -0.0 5,818,405 + PP_5094 0.58 -1.2 5,818,405 + PP_5094 0.58 -0.9 5,818,406 - PP_5094 0.58 -2.7 5,818,431 + PP_5094 0.62 +0.5 5,818,746 + -2.8 5,818,746 + -1.2 5,818,746 + -0.2 5,818,747 - -1.1 5,818,747 - -0.8 5,818,747 - +0.6 5,818,747 - -1.2 5,818,747 - -1.1 5,818,747 - +0.0 5,818,968 - PP_5095 0.29 -0.2 5,819,128 + PP_5095 0.48 +0.4 5,819,129 - PP_5095 0.48 +0.0 5,819,190 - PP_5095 0.56 -0.2 5,819,548 + +0.3 5,819,548 + -0.7 5,819,549 - -3.0 5,819,558 + -0.0 5,819,558 + -0.0 5,819,558 + +1.2 5,819,558 + -1.4 5,819,558 + -0.4 5,819,558 + -0.9 5,819,559 - -1.7 5,819,559 - -1.0 5,819,669 + PP_5096 0.18 +0.3 5,819,669 + PP_5096 0.18 -1.6 5,819,670 - PP_5096 0.18 -1.1 5,819,670 - PP_5096 0.18 -0.6 5,819,670 - PP_5096 0.18 -0.5 5,819,740 + PP_5096 0.30 -0.8 5,819,825 + PP_5096 0.45 -0.6 5,819,825 + PP_5096 0.45 +0.4 5,819,826 - PP_5096 0.45 +0.2 5,819,826 - PP_5096 0.45 +0.3 5,819,826 - PP_5096 0.45 -1.5 5,819,826 - PP_5096 0.45 +0.3 5,819,830 + PP_5096 0.46 +1.7 5,819,830 + PP_5096 0.46 -0.6 5,819,831 - PP_5096 0.46 -0.8 5,819,831 - PP_5096 0.46 -0.8 5,820,216 + -0.5 5,820,222 + -1.0 5,820,222 + +0.0 5,820,222 + -3.2 5,820,222 + -3.5 5,820,223 - -1.1 5,820,289 - -3.8 5,820,354 - -3.1 5,820,366 - PP_5097 0.10 -2.6 5,820,374 + PP_5097 0.11 -2.5 5,820,374 + PP_5097 0.11 -2.7 5,820,374 + PP_5097 0.11 -2.3 5,820,374 + PP_5097 0.11 -2.6 5,820,374 + PP_5097 0.11 -3.4 5,820,374 + PP_5097 0.11 -2.4 5,820,375 - PP_5097 0.11 -2.7 5,820,375 - PP_5097 0.11 -1.2 5,820,375 - PP_5097 0.11 -2.0 5,820,375 - PP_5097 0.11 +0.4 5,820,375 - PP_5097 0.11 -3.8 5,820,375 - PP_5097 0.11 -4.1 5,820,375 - PP_5097 0.11 -2.9 5,820,452 + PP_5097 0.18 -0.1
Or see this region's nucleotide sequence