Strain Fitness in Pseudomonas putida KT2440 around PP_4375

Experiment: Growth at pH8 and (C) D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4373 and PP_4374 are separated by 170 nucleotidesPP_4374 and PP_4375 are separated by 18 nucleotidesPP_4375 and PP_4376 are separated by 82 nucleotides PP_4373: PP_4373 - Transcriptional regulator FleQ, at 4,963,882 to 4,965,357 _4373 PP_4374: PP_4374 - flagellar protein, at 4,965,528 to 4,965,821 _4374 PP_4375: PP_4375 - flagellar chaperone, inhibits premature FliC assembly, at 4,965,840 to 4,966,235 _4375 PP_4376: PP_4376 - flagellar filament capping protein, at 4,966,318 to 4,967,676 _4376 Position (kb) 4965 4966 4967Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4964.975 kb on + strand, within PP_4373at 4964.975 kb on + strand, within PP_4373at 4964.975 kb on + strandat 4964.976 kb on - strand, within PP_4373at 4964.976 kb on - strand, within PP_4373at 4965.026 kb on + strand, within PP_4373at 4965.027 kb on - strand, within PP_4373at 4965.027 kb on - strand, within PP_4373at 4965.136 kb on - strand, within PP_4373at 4965.148 kb on - strand, within PP_4373at 4965.363 kb on - strandat 4965.363 kb on - strandat 4965.363 kb on - strandat 4965.363 kb on - strandat 4965.466 kb on + strandat 4965.466 kb on + strandat 4965.466 kb on + strandat 4965.466 kb on + strandat 4965.467 kb on - strandat 4965.481 kb on - strandat 4965.544 kb on + strandat 4965.544 kb on + strandat 4965.544 kb on + strandat 4965.544 kb on + strandat 4965.544 kb on + strandat 4965.545 kb on - strandat 4965.545 kb on - strandat 4965.545 kb on - strandat 4965.545 kb on - strandat 4965.546 kb on + strandat 4965.546 kb on + strandat 4965.547 kb on - strandat 4965.547 kb on - strandat 4965.547 kb on - strandat 4965.575 kb on + strand, within PP_4374at 4965.703 kb on - strand, within PP_4374at 4965.832 kb on + strandat 4965.833 kb on + strandat 4965.834 kb on - strandat 4965.838 kb on + strandat 4965.841 kb on + strandat 4965.841 kb on + strandat 4965.841 kb on + strandat 4965.841 kb on + strandat 4965.841 kb on + strandat 4965.842 kb on - strandat 4965.927 kb on - strand, within PP_4375at 4965.961 kb on + strand, within PP_4375at 4965.961 kb on + strand, within PP_4375at 4965.961 kb on + strand, within PP_4375at 4965.961 kb on + strand, within PP_4375at 4965.961 kb on + strand, within PP_4375at 4965.962 kb on - strand, within PP_4375at 4965.962 kb on - strand, within PP_4375at 4965.962 kb on - strand, within PP_4375at 4965.967 kb on + strand, within PP_4375at 4965.967 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.041 kb on + strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.042 kb on - strand, within PP_4375at 4966.043 kb on + strand, within PP_4375at 4966.043 kb on + strand, within PP_4375at 4966.044 kb on - strand, within PP_4375at 4966.044 kb on - strand, within PP_4375at 4966.044 kb on - strand, within PP_4375at 4966.044 kb on - strand, within PP_4375at 4966.044 kb on - strand, within PP_4375at 4966.068 kb on + strand, within PP_4375at 4966.068 kb on + strand, within PP_4375at 4966.068 kb on + strand, within PP_4375at 4966.068 kb on + strand, within PP_4375at 4966.068 kb on + strand, within PP_4375at 4966.069 kb on - strand, within PP_4375at 4966.069 kb on - strand, within PP_4375at 4966.069 kb on - strand, within PP_4375at 4966.260 kb on - strandat 4966.270 kb on + strandat 4966.270 kb on + strandat 4966.270 kb on + strandat 4966.270 kb on + strandat 4966.270 kb on + strandat 4966.270 kb on + strandat 4966.274 kb on + strandat 4966.274 kb on + strandat 4966.274 kb on + strandat 4966.274 kb on + strandat 4966.275 kb on - strandat 4966.275 kb on - strandat 4966.278 kb on + strandat 4966.278 kb on + strandat 4966.278 kb on + strandat 4966.278 kb on + strandat 4966.278 kb on + strandat 4966.278 kb on + strandat 4966.279 kb on - strandat 4966.279 kb on - strandat 4966.279 kb on - strandat 4966.311 kb on - strandat 4966.311 kb on - strandat 4966.415 kb on + strandat 4966.415 kb on + strandat 4966.415 kb on + strandat 4966.416 kb on - strandat 4966.416 kb on - strandat 4966.449 kb on + strandat 4966.449 kb on + strandat 4966.450 kb on - strandat 4966.487 kb on + strand, within PP_4376at 4966.489 kb on + strand, within PP_4376at 4966.489 kb on + strand, within PP_4376at 4966.544 kb on + strand, within PP_4376at 4966.544 kb on + strand, within PP_4376at 4966.544 kb on + strand, within PP_4376at 4966.545 kb on - strand, within PP_4376at 4966.545 kb on - strand, within PP_4376at 4966.545 kb on - strand, within PP_4376at 4966.545 kb on - strand, within PP_4376at 4966.683 kb on + strand, within PP_4376at 4966.806 kb on + strand, within PP_4376at 4966.807 kb on - strand, within PP_4376at 4966.807 kb on - strand, within PP_4376at 4966.871 kb on + strand, within PP_4376at 4966.872 kb on - strand, within PP_4376at 4966.872 kb on - strand, within PP_4376at 4966.872 kb on - strand, within PP_4376at 4966.908 kb on + strand, within PP_4376at 4966.909 kb on - strand, within PP_4376at 4966.909 kb on - strand, within PP_4376at 4966.914 kb on + strand, within PP_4376at 4966.915 kb on - strand, within PP_4376at 4966.915 kb on - strand, within PP_4376at 4966.915 kb on - strand, within PP_4376at 4966.915 kb on - strand, within PP_4376at 4966.942 kb on - strand, within PP_4376at 4966.942 kb on - strand, within PP_4376at 4966.951 kb on + strand, within PP_4376at 4966.951 kb on + strand, within PP_4376at 4966.952 kb on - strand, within PP_4376at 4966.992 kb on + strand, within PP_4376at 4966.993 kb on - strand, within PP_4376at 4966.993 kb on - strand, within PP_4376at 4966.993 kb on - strand, within PP_4376at 4966.995 kb on + strandat 4966.996 kb on - strand, within PP_4376at 4966.996 kb on - strand, within PP_4376at 4967.030 kb on + strand, within PP_4376at 4967.098 kb on - strand, within PP_4376at 4967.134 kb on + strand, within PP_4376at 4967.134 kb on + strand, within PP_4376at 4967.199 kb on + strand, within PP_4376

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose
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4,964,975 + PP_4373 0.74 +0.1
4,964,975 + PP_4373 0.74 +1.6
4,964,975 + +1.1
4,964,976 - PP_4373 0.74 +1.1
4,964,976 - PP_4373 0.74 +1.7
4,965,026 + PP_4373 0.78 +1.7
4,965,027 - PP_4373 0.78 +1.4
4,965,027 - PP_4373 0.78 +1.4
4,965,136 - PP_4373 0.85 +1.5
4,965,148 - PP_4373 0.86 +1.5
4,965,363 - +1.4
4,965,363 - +1.7
4,965,363 - +1.5
4,965,363 - +1.2
4,965,466 + +1.5
4,965,466 + +1.6
4,965,466 + +1.7
4,965,466 + +1.4
4,965,467 - -0.2
4,965,481 - +0.1
4,965,544 + +1.8
4,965,544 + +1.7
4,965,544 + +1.4
4,965,544 + +0.1
4,965,544 + +0.7
4,965,545 - +0.4
4,965,545 - -2.5
4,965,545 - +0.3
4,965,545 - +1.9
4,965,546 + +1.7
4,965,546 + +3.5
4,965,547 - -3.6
4,965,547 - -0.4
4,965,547 - +1.2
4,965,575 + PP_4374 0.16 +1.3
4,965,703 - PP_4374 0.60 -1.2
4,965,832 + +0.8
4,965,833 + +1.1
4,965,834 - -0.2
4,965,838 + -0.3
4,965,841 + +0.6
4,965,841 + +0.6
4,965,841 + +0.5
4,965,841 + +0.6
4,965,841 + -0.1
4,965,842 - +1.3
4,965,927 - PP_4375 0.22 +1.1
4,965,961 + PP_4375 0.31 +1.9
4,965,961 + PP_4375 0.31 +2.1
4,965,961 + PP_4375 0.31 +2.3
4,965,961 + PP_4375 0.31 -1.1
4,965,961 + PP_4375 0.31 +0.2
4,965,962 - PP_4375 0.31 +2.3
4,965,962 - PP_4375 0.31 +0.9
4,965,962 - PP_4375 0.31 +1.1
4,965,967 + PP_4375 0.32 -0.4
4,965,967 + PP_4375 0.32 +1.2
4,966,041 + PP_4375 0.51 -1.8
4,966,041 + PP_4375 0.51 +1.6
4,966,041 + PP_4375 0.51 -0.2
4,966,041 + PP_4375 0.51 +1.3
4,966,041 + PP_4375 0.51 +0.8
4,966,041 + PP_4375 0.51 +1.6
4,966,041 + PP_4375 0.51 -0.5
4,966,041 + PP_4375 0.51 +2.5
4,966,042 - PP_4375 0.51 +0.5
4,966,042 - PP_4375 0.51 -0.9
4,966,042 - PP_4375 0.51 +1.5
4,966,042 - PP_4375 0.51 +0.5
4,966,042 - PP_4375 0.51 +0.3
4,966,042 - PP_4375 0.51 -0.1
4,966,042 - PP_4375 0.51 +2.1
4,966,042 - PP_4375 0.51 +0.4
4,966,043 + PP_4375 0.51 +2.3
4,966,043 + PP_4375 0.51 +1.8
4,966,044 - PP_4375 0.52 +2.7
4,966,044 - PP_4375 0.52 +1.3
4,966,044 - PP_4375 0.52 -0.4
4,966,044 - PP_4375 0.52 +0.3
4,966,044 - PP_4375 0.52 +1.2
4,966,068 + PP_4375 0.58 -2.3
4,966,068 + PP_4375 0.58 -1.9
4,966,068 + PP_4375 0.58 +0.1
4,966,068 + PP_4375 0.58 +0.1
4,966,068 + PP_4375 0.58 +2.6
4,966,069 - PP_4375 0.58 +1.7
4,966,069 - PP_4375 0.58 +0.5
4,966,069 - PP_4375 0.58 -1.2
4,966,260 - +0.2
4,966,270 + -2.1
4,966,270 + -1.1
4,966,270 + +1.3
4,966,270 + +1.0
4,966,270 + +0.5
4,966,270 + -0.7
4,966,274 + +0.9
4,966,274 + -2.1
4,966,274 + -0.9
4,966,274 + +0.4
4,966,275 - +0.5
4,966,275 - -3.5
4,966,278 + +2.1
4,966,278 + +2.4
4,966,278 + +0.8
4,966,278 + +2.3
4,966,278 + +0.5
4,966,278 + +2.4
4,966,279 - -1.1
4,966,279 - +0.9
4,966,279 - -3.4
4,966,311 - +0.3
4,966,311 - +1.5
4,966,415 + +0.5
4,966,415 + +1.6
4,966,415 + +1.5
4,966,416 - +0.4
4,966,416 - -0.2
4,966,449 + +0.1
4,966,449 + +1.6
4,966,450 - +2.0
4,966,487 + PP_4376 0.12 +0.1
4,966,489 + PP_4376 0.13 -0.7
4,966,489 + PP_4376 0.13 -0.4
4,966,544 + PP_4376 0.17 -0.7
4,966,544 + PP_4376 0.17 -0.2
4,966,544 + PP_4376 0.17 +1.4
4,966,545 - PP_4376 0.17 -0.9
4,966,545 - PP_4376 0.17 +1.4
4,966,545 - PP_4376 0.17 -0.2
4,966,545 - PP_4376 0.17 -0.0
4,966,683 + PP_4376 0.27 +0.7
4,966,806 + PP_4376 0.36 +1.6
4,966,807 - PP_4376 0.36 +0.3
4,966,807 - PP_4376 0.36 +2.3
4,966,871 + PP_4376 0.41 +1.7
4,966,872 - PP_4376 0.41 -2.9
4,966,872 - PP_4376 0.41 -0.5
4,966,872 - PP_4376 0.41 +0.8
4,966,908 + PP_4376 0.43 +0.8
4,966,909 - PP_4376 0.43 -2.5
4,966,909 - PP_4376 0.43 +0.5
4,966,914 + PP_4376 0.44 +1.8
4,966,915 - PP_4376 0.44 +0.9
4,966,915 - PP_4376 0.44 +0.7
4,966,915 - PP_4376 0.44 +1.4
4,966,915 - PP_4376 0.44 +1.4
4,966,942 - PP_4376 0.46 -0.1
4,966,942 - PP_4376 0.46 -0.3
4,966,951 + PP_4376 0.47 -0.1
4,966,951 + PP_4376 0.47 +0.3
4,966,952 - PP_4376 0.47 +1.0
4,966,992 + PP_4376 0.50 -0.6
4,966,993 - PP_4376 0.50 -0.5
4,966,993 - PP_4376 0.50 -1.4
4,966,993 - PP_4376 0.50 +0.4
4,966,995 + +2.9
4,966,996 - PP_4376 0.50 +1.1
4,966,996 - PP_4376 0.50 +2.0
4,967,030 + PP_4376 0.52 -0.7
4,967,098 - PP_4376 0.57 +1.6
4,967,134 + PP_4376 0.60 -0.1
4,967,134 + PP_4376 0.60 +1.3
4,967,199 + PP_4376 0.65 +1.5

Or see this region's nucleotide sequence