Experiment: Growth at pH8 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5450 and PP_5451 are separated by 374 nucleotides PP_5451 and PP_0641 are separated by 169 nucleotides
PP_5450: PP_5450 - exported protein of unknown function, at 751,007 to 751,606
_5450
PP_5451: PP_5451 - protein of unknown function, at 751,981 to 752,286
_5451
PP_0641: PP_0641 - conserved protein of unknown function, at 752,456 to 752,761
_0641
Position (kb) 751
752
753 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 750.994 kb on + strand at 750.994 kb on + strand at 751.004 kb on + strand at 751.008 kb on + strand at 751.134 kb on + strand, within PP_5450 at 751.134 kb on + strand, within PP_5450 at 751.134 kb on + strand, within PP_5450 at 751.134 kb on + strand, within PP_5450 at 751.135 kb on - strand, within PP_5450 at 751.145 kb on + strand, within PP_5450 at 751.145 kb on + strand, within PP_5450 at 751.277 kb on - strand, within PP_5450 at 751.284 kb on - strand, within PP_5450 at 751.372 kb on + strand, within PP_5450 at 751.372 kb on + strand, within PP_5450 at 751.373 kb on - strand, within PP_5450 at 751.373 kb on - strand, within PP_5450 at 751.373 kb on - strand at 751.392 kb on - strand, within PP_5450 at 751.402 kb on + strand, within PP_5450 at 751.403 kb on - strand, within PP_5450 at 751.476 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.489 kb on - strand, within PP_5450 at 751.513 kb on + strand, within PP_5450 at 751.555 kb on + strand at 751.556 kb on - strand at 751.556 kb on - strand at 751.556 kb on - strand at 751.580 kb on + strand at 751.609 kb on - strand at 751.609 kb on - strand at 751.621 kb on + strand at 751.622 kb on - strand at 751.647 kb on + strand at 751.647 kb on + strand at 751.648 kb on - strand at 751.648 kb on - strand at 751.752 kb on - strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.771 kb on - strand at 751.771 kb on - strand at 751.797 kb on - strand at 751.829 kb on + strand at 751.830 kb on - strand at 751.903 kb on - strand at 751.903 kb on - strand at 751.911 kb on + strand at 751.911 kb on + strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.925 kb on - strand at 751.925 kb on - strand at 751.927 kb on + strand at 751.927 kb on + strand at 751.927 kb on + strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.992 kb on - strand at 751.992 kb on - strand at 751.992 kb on - strand at 752.006 kb on + strand at 752.089 kb on + strand, within PP_5451 at 752.090 kb on - strand, within PP_5451 at 752.090 kb on - strand, within PP_5451 at 752.090 kb on - strand, within PP_5451 at 752.127 kb on - strand, within PP_5451 at 752.173 kb on + strand, within PP_5451 at 752.174 kb on - strand, within PP_5451 at 752.264 kb on + strand at 752.284 kb on + strand at 752.352 kb on - strand at 752.427 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.550 kb on + strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.628 kb on - strand, within PP_0641 at 752.700 kb on + strand, within PP_0641 at 752.700 kb on + strand, within PP_0641 at 752.701 kb on - strand, within PP_0641 at 752.701 kb on - strand, within PP_0641 at 752.701 kb on - strand at 752.701 kb on - strand, within PP_0641 at 752.701 kb on - strand, within PP_0641 at 752.745 kb on - strand at 752.849 kb on + strand at 752.850 kb on - strand at 752.850 kb on - strand at 752.942 kb on + strand at 752.976 kb on + strand at 752.976 kb on + strand at 752.977 kb on - strand at 752.977 kb on - strand at 753.005 kb on + strand at 753.005 kb on + strand at 753.005 kb on + strand at 753.006 kb on - strand at 753.009 kb on + strand at 753.009 kb on + strand at 753.071 kb on + strand at 753.071 kb on + strand at 753.072 kb on - strand at 753.206 kb on + strand at 753.207 kb on - strand at 753.207 kb on - strand at 753.207 kb on - strand at 753.216 kb on - strand at 753.222 kb on + strand at 753.223 kb on - strand at 753.228 kb on - strand at 753.231 kb on + strand at 753.231 kb on + strand at 753.231 kb on + strand at 753.232 kb on - strand at 753.238 kb on + strand at 753.239 kb on - strand at 753.241 kb on + strand at 753.241 kb on + strand at 753.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) D-Glucose remove 750,994 + -0.1 750,994 + +0.6 751,004 + +1.3 751,008 + +0.9 751,134 + PP_5450 0.21 -0.9 751,134 + PP_5450 0.21 -0.1 751,134 + PP_5450 0.21 +1.1 751,134 + PP_5450 0.21 +0.4 751,135 - PP_5450 0.21 +0.6 751,145 + PP_5450 0.23 +0.6 751,145 + PP_5450 0.23 -4.2 751,277 - PP_5450 0.45 -1.0 751,284 - PP_5450 0.46 +0.4 751,372 + PP_5450 0.61 -0.0 751,372 + PP_5450 0.61 +0.5 751,373 - PP_5450 0.61 +0.2 751,373 - PP_5450 0.61 -0.2 751,373 - -1.1 751,392 - PP_5450 0.64 +2.0 751,402 + PP_5450 0.66 -0.2 751,403 - PP_5450 0.66 -0.3 751,476 - PP_5450 0.78 -2.4 751,480 - PP_5450 0.79 +1.1 751,480 - PP_5450 0.79 +0.6 751,480 - PP_5450 0.79 +0.9 751,489 - PP_5450 0.80 -0.3 751,513 + PP_5450 0.84 +0.9 751,555 + -0.2 751,556 - -0.3 751,556 - -0.5 751,556 - -2.3 751,580 + -0.0 751,609 - +0.1 751,609 - -1.6 751,621 + +0.1 751,622 - -0.8 751,647 + +0.8 751,647 + +0.4 751,648 - -2.3 751,648 - -2.5 751,752 - +0.4 751,770 + -0.1 751,770 + +0.4 751,771 - -1.9 751,771 - +1.7 751,797 - +0.5 751,829 + +0.4 751,830 - +0.8 751,903 - +0.1 751,903 - +0.6 751,911 + +1.5 751,911 + -0.7 751,912 - -0.6 751,912 - -1.4 751,912 - +0.5 751,912 - -1.1 751,912 - +0.4 751,912 - +0.4 751,916 - -0.1 751,916 - -0.8 751,916 - -2.8 751,916 - -1.0 751,916 - +0.2 751,925 - +1.9 751,925 - +1.6 751,927 + -0.1 751,927 + +0.5 751,927 + -0.3 751,928 - +1.5 751,928 - +1.1 751,928 - -1.1 751,928 - -1.1 751,928 - -0.4 751,992 - +1.0 751,992 - +0.8 751,992 - -1.6 752,006 + +0.9 752,089 + PP_5451 0.35 +0.3 752,090 - PP_5451 0.36 +0.8 752,090 - PP_5451 0.36 -3.0 752,090 - PP_5451 0.36 -1.6 752,127 - PP_5451 0.48 -3.4 752,173 + PP_5451 0.63 -1.4 752,174 - PP_5451 0.63 -1.1 752,264 + +0.6 752,284 + -0.7 752,352 - +0.7 752,427 + +0.8 752,484 + -0.0 752,484 + -0.7 752,484 + +0.6 752,484 + +0.8 752,484 + +0.6 752,485 - +0.6 752,485 - -0.7 752,485 - +0.4 752,485 - +0.1 752,485 - -0.9 752,487 + PP_0641 0.10 +1.2 752,487 + PP_0641 0.10 +1.1 752,487 + PP_0641 0.10 -0.5 752,487 + PP_0641 0.10 +0.5 752,487 + PP_0641 0.10 +1.4 752,488 - PP_0641 0.10 -0.4 752,488 - PP_0641 0.10 +0.5 752,488 - PP_0641 0.10 +0.4 752,488 - PP_0641 0.10 +0.0 752,488 - PP_0641 0.10 -0.2 752,488 - PP_0641 0.10 -0.4 752,550 + PP_0641 0.31 +0.3 752,551 - PP_0641 0.31 +2.3 752,551 - PP_0641 0.31 -0.9 752,551 - PP_0641 0.31 -1.3 752,551 - PP_0641 0.31 -1.1 752,551 - PP_0641 0.31 -0.1 752,628 - PP_0641 0.56 +1.0 752,700 + PP_0641 0.80 +0.6 752,700 + PP_0641 0.80 -0.4 752,701 - PP_0641 0.80 +0.2 752,701 - PP_0641 0.80 -0.1 752,701 - -1.3 752,701 - PP_0641 0.80 -3.7 752,701 - PP_0641 0.80 +0.5 752,745 - +0.1 752,849 + +1.0 752,850 - +0.3 752,850 - -0.3 752,942 + +1.6 752,976 + +1.4 752,976 + +1.2 752,977 - -0.4 752,977 - +0.0 753,005 + +0.2 753,005 + -0.9 753,005 + +0.6 753,006 - -0.0 753,009 + -1.0 753,009 + -1.1 753,071 + +0.1 753,071 + +0.5 753,072 - -0.7 753,206 + +0.6 753,207 - -0.2 753,207 - -0.9 753,207 - -0.0 753,216 - -0.1 753,222 + +0.3 753,223 - -0.2 753,228 - +0.2 753,231 + +1.1 753,231 + -1.3 753,231 + -0.3 753,232 - +0.1 753,238 + -0.1 753,239 - +0.8 753,241 + +0.4 753,241 + +1.0 753,251 + -1.2
Or see this region's nucleotide sequence